Separation of Anisoles

Column	Chromolith® Performance Si 100-4.6 mm
Mobile phase	n-Heptane/Dioxane 95/5 v/v
Flow rate	2 mL/min
Pressure	14 bar
Detection	UV 254 nm
Temperature	Ambient
Inj. volume	5 µL
Sample	1.Anisole                                        2. 3-Nitroanisole 3. 4-Nitroanisole 4. 2-Nitroanisole	0.39 mg/mL 0.07 mg/mL 0.26 mg/mL 0.18 mg/mL