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219360 Cathepsin D/E Substrate, Fluorogenic - Calbiochem

219360
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219360-1MG
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      Ampoule plast. 1 mg
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      Description
      OverviewAn internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates.
      Catalogue Number219360
      Brand Family Calbiochem®
      SynonymsMOCAc-GKPILF~FRLK(Dnp)-D-R-NH₂
      References
      ReferencesYasuda, Y., et al. 1999. J. Biochem. 126, 260.
      Yasuda, Y., et al. 1999. J. Biochem. 125, 1137.
      Product Information
      ATP CompetitiveN
      FormYellow lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₈₅H₁₂₂N₂₂O₁₉
      Chemical formulaC₈₅H₁₂₂N₂₂O₁₉
      Hygroscopic Hygroscopic
      ReversibleN
      Quality LevelMQ100
      Applications
      ApplicationCathepsin D/E Substrate, Fluorogenic, is an internally quenched fluorogenic 11-mer peptide that acts as a selective substrate for cathepsins D and E.
      Biological Information
      Primary TargetSubstrate for cathepsins D and E
      Primary Target IC<sub>50</sub>kcat/Km = 15.6 µM⁻¹ sec⁻¹ and 10.9 µM⁻¹ sec⁻¹ for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively
      Purity≥97% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Peptide SequenceMOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂
      MOCAc = (7-Methoxycoumarin-4-yl)acetyl
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Cathepsin D/E Substrate, Fluorogenic - Calbiochem Certificats d'analyse

      TitreNuméro de lot
      219360

      Références bibliographiques

      Aperçu de la référence bibliographique
      Yasuda, Y., et al. 1999. J. Biochem. 126, 260.
      Yasuda, Y., et al. 1999. J. Biochem. 125, 1137.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-June-2008 RFH
      SynonymsMOCAc-GKPILF~FRLK(Dnp)-D-R-NH₂
      DescriptionAn internally quenched fluorogenic 11 amino acid peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively). Does not act as a substrate for cathepsins B, H, or L. Cleavage occurs at the Phe-Phe amide bond resulting in enhanced fluorescence. Useful for screening cathepsin D and E inhibitors and for determining cathepsin D and E activity in tissue and cell extracts. Excitation max: ~328 nm; emission max: ~393 nm.
      FormYellow lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Recommended reaction conditions80 µl of 50 mM sodium acetate buffer, pH 4.0, 10 µl of 200 µM substrate solution, 10 µl of sample, incubate at 40°C for 10 min; terminate reaction with 2 ml of 5% TCA.
      Chemical formulaC₈₅H₁₂₂N₂₂O₁₉
      Peptide SequenceMOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂
      MOCAc = (7-Methoxycoumarin-4-yl)acetyl
      Purity≥97% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage +2°C to +8°C
      Hygroscopic
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesYasuda, Y., et al. 1999. J. Biochem. 126, 260.
      Yasuda, Y., et al. 1999. J. Biochem. 125, 1137.