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93283

Tris-EDTA buffer solution

Name: Tris-EDTA buffer solution
Brand: SIAL

BioUltra, Molecular Biology, pH 8.0

동의어(들):

TE buffer, TE buffer solution

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:

41105319

eCl@ss:

32129211

PubChem Substance ID:

329770217

NACRES:

NA.25

MDL number:

MFCD00236359

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도움 문의

속성

grade

Molecular Biology

Quality Segment

200

product line

BioUltra

form

liquid

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

pH

8.0±0.2 (25 °C)

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm A_max: 0.04, λ: 280 nm A_max: 0.01

application(s)

sample preparation

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

설명

General description

Tris EDTA is a biological buffer in research. It is a combination of Tris base and EDTA, a metal chelator. Tris provides buffering capacity, maintaining a stable pH in biological samples, while EDTA helps prevent metal ion interference by chelating divalent metal ions. Tris EDTA is often used in molecular biology techniques, such as DNA and RNA sample preparation, electrophoresis, and enzymatic reactions. It helps maintain the stability and integrity of nucleic acids by inhibiting the activity of nucleases and metal-dependent enzymes.

Application

TE buffer is useful for general extraction, suspension, washing, and storage buffer and in the preparation of DNA or RNA. Commonly used for DNA sequencing, restriction enzyme digestion; ligation and polymerase chain reaction (PCR) applications.

Features and Benefits

BioUltra grade - suitable for many molecular biology applications
DNase, RNase, phosphatase, and protease-free
High purity and free of trace metals

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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저장 등급

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c