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Merck

F7425

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-ddddk, Anti-dykddddk

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.32
MDL number:
MFCD02262912

Nom du produit

ANTI-FLAG® antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

all

concentration

~0.8 mg/mL

technique(s)

dot blot: 1-2.5 μg/mL
immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Catégories apparentées

Application

L'anticorps reconnaît l'épitope FLAG situé au niveau de l'extrémité C-terminale ou N-terminale des protéines de fusion avec étiquette FLAG, lors d'essais du type dot blot, immunoblotting, immunoprécipitation et immunocytochimie.
Pour en savoir plus sur nos produits, rendez-vous sur notre portail des applications des produits FLAG®.

Biochem/physiol Actions

L′épitope FLAG joue un rôle crucial dans la purification par immuno-affinité des protéines de fusion.

General description

L′anticorps polyclonal anti-FLAG de lapin reconnaît l′épitope FLAG situé sur les protéines de fusion avec une étiquette FLAG. Cet anticorps réagit avec les protéines de fusion portant une étiquette FLAG sur l′extrémité N-terminale, Met-N-terminale ou C-terminale.
L′épitope FLAG est une protéine de huit acides aminés dotée d′un site de clivage de l′entérokinase.

Immunogen

FLAG ; séquence peptidique DYKDDDDK

Other Notes

Lauréat du prix CiteAb 2024 récompensant les avancées des fournisseurs dans la recherche sur la maladie de Parkinson

Physical form

Solution dans une solution saline tamponnée au phosphate 0,01 M à pH 7,4, contenant 1 % d′albumine de sérum bovin et de l′azide de sodium 15 mM

Preparation Note

Produit purifié par chromatographie d′affinité sur une colonne greffée avec le peptide immunisant.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Classe de stockage

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000485141
0000485141
0000422515
0000422515
0000395474

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

  1. Is Product F7425, ANTI-FLAG® from rabbit, a monoclonal or polyclonal?

    F7425 is a polyclonal antibody from rabbit.

  2. What is the concentration of Product F7425, ANTI-FLAG® from rabbit?

    The concentration is 0.9 mg protein per ml.

  3. How much Product F7425, ANTI-FLAG® from rabbit, should be used in western blotting and ELISA applications?

    For a Western blot from a PAGE run (preferably) under denaturing conditions, a 1:400 dilution of this antibody should be used.  A 1-10 μg/ml concentration should be used when using thie antibody in an ELISA application.

  4. How to get rid of background when using Product F7465, ANTI-FLAG® from rabbit,?

    To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation buffers. Try a Triton X-100 concentration of 0.1% to start. Tween 20 (Product No. P5927) can also be used.

  5. Is there any references for the use of Product F7465, ANTI-FLAG® from rabbit, in immunohistochemistry or western blotting?

    K. Schafer and T. Braun, Monoclonal Anti-FLAG Antibodies React with a New Isoform of Rat Mg++ Dependent Protein Phosphatase. Biochemical and Biophysical Research Communications, 207, 708.

  6. What are the positive controls that can be used with Product F7425, ANTI-FLAG® from rabbit?

    The positive controls that can be used are as follows:Amino-terminal FLAG-BAP fusion protein, Product No. P7582Carboxy-terminal FLAG-BAP fusion protein, Product No. P7457Amino-terminal Met-FLAG-BAP fusion protein, Product No. P5975.

  7. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Ryota Sato et al.
Frontiers in microbiology, 10, 1857-1857 (2019-08-29)
The envelope proteins of influenza A virus, hemagglutinin (HA) and neuraminidase (NA), play critical roles in viral entry to host cells and release from the cells, respectively. After protein synthesis, they are transported from the trans-Golgi network (TGN) to the
Martin S Taylor et al.
The Journal of biological chemistry, 288(45), 32211-32228 (2013-09-21)
Ghrelin O-acyltransferase (GOAT) is a polytopic integral membrane protein required for activation of ghrelin, a secreted metabolism-regulating peptide hormone. Although GOAT is a potential therapeutic target for the treatment of obesity and diabetes and plays a key role in other
Makoto Miyazawa et al.
The Journal of biological chemistry, 286(22), 19191-19203 (2011-04-12)
The molecular chaperone prefoldin (PFD) is a complex comprised of six different subunits, PFD1-PFD6, and delivers newly synthesized unfolded proteins to cytosolic chaperonin TRiC/CCT to facilitate the folding of proteins. PFD subunits also have functions different from the function of
Ecco Staller et al.
Journal of virology, 93(17) (2019-06-21)
ANP32 proteins have been implicated in supporting influenza virus replication, but most of the work to date has focused on the ability of avian Anp32 proteins to overcome restriction of avian influenza polymerases in human cells. Using a CRISPR approach
Dorothee A Vogt et al.
PLoS pathogens, 9(4), e1003302-e1003302 (2013-04-18)
The nonstructural protein NS5A has emerged as a new drug target in antiviral therapies for Hepatitis C Virus (HCV) infection. NS5A is critically involved in viral RNA replication that takes place at newly formed membranes within the endoplasmic reticulum (membranous
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