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FLAGIPT1

FLAG® Immunoprecipitation Kit

Synonyme(s) :

Anti-ddddk, Anti-dykddddk

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A propos de cet article

NACRES:
NA.32
UNSPSC Code:
12352200
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usage

 kit sufficient for 50 reactions

Quality Level

300

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

storage temp.

−20°C

General description

Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Application

Learn more product details in our FLAG® application portal.

Features and Benefits

  • Utilizes highly specific ANTI-FLAG M2 affinity gel
  • No preliminary steps or calibrations
  • 3xFLAG™ peptide provides efficient and gentle elution through direct competition

Other Notes

All prodedures to be performed at 2-8°C.

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS & Tarif
  • A2220
    ANTI-FLAG M2 affinity gel 1 mL
    FDS
  • SAE0194
    3X FLAG peptide 1 mg
    FDS

pictograms

Exclamation mark
GHS07

Classe de stockage

10 - Combustible liquids

wgk

WGK 3

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2

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Certificats d'analyse (COA)

Lot/Batch Number
0000527455
0000527455
0000162028
0000162028
0000162024

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Andrew S Terker et al.
American journal of physiology. Renal physiology, 315(4), F781-F790 (2018-02-08)
With no lysine kinase 4 (WNK4) is essential to activate the thiazide-sensitive NaCl cotransporter (NCC) along the distal convoluted tubule, an effect central to the phenotype of familial hyperkalemic hypertension. Although effects on potassium and sodium channels along the connecting
Mateusz Wydro et al.
Nucleic acids research, 38(11), 3732-3742 (2010-02-11)
Mammalian mitochondria contain their own genome that is almost fully transcribed from both strands, generating polycistronic RNA units that are processed and matured. The mitochondrial mRNA is modified by oligo- or polyadenylation at the 3' termini, but the exact function
Samer Swedan et al.
Journal of virology, 85(19), 10090-10100 (2011-07-29)
Human respiratory syncytial virus (RSV), a major cause of severe respiratory diseases, efficiently suppresses cellular innate immunity, represented by type I interferon (IFN), using its two unique nonstructural proteins, NS1 and NS2. In a search for their mechanism, NS1 was
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