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MAB5300 Anti-Dopamine Antibody, clone K56A

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100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RIHCMPurifiedMonoclonal Antibody
Catalogue NumberMAB5300
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Dopamine Antibody, clone K56A
Product Information
PresentationPurified immunoglobulin. Liquid containing 50% glycerol.
Quality LevelMQ100
ApplicationAnti-Dopamine Antibody, clone K56A detects level of Dopamine & has been published & validated for use in IH.
Key Applications
  • Immunohistochemistry
Application NotesImmunohistochemistry: 1:500-1:2,500 using free floating sections by the PAP technique on rat dopaminergic areas.

Optimal working dilutions must be determined by end user.

PROTOCOL for Dopamine Detection by Immunohisto/cytochemistry. Example for a rat brain.

1. SOLUTIONS TO BE PREPARED - Solution must be prepared as needed.

Solution A: 0.1M cacodylate, 10g/L sodium metabisulfite, pH 6.2.

Solution B: 0.1M cacodylate, 10g/L sodium metabisulfite, 3-5% glutaraldehyde, pH 7.5.

2. RAT PERFUSION - The rat is anaesthetized with sodium pentobarbital or Nembutal and perfused intracardially through the aorta using a pump with Solution A (30 mL): 150-300 mL/min, Solution B (500 mL): 150-300 mL/min.

3. POST FIXATION: 15 to 30 minutes in Solution B, then 4 soft washes in 0.05M Tris with 8.5 g/L sodium metabisulfite, pH 7.5 (Solution C) .

4. TISSUE SECTIONING: Vibratome or cryostat sections can be used.

5. REDUCTION STEP: Sections are reduced with Solution C containing 0.1M sodium borohydride for 10 minutes. The sections are washed 4 times in solution C without sodium borohydride.

6. APPLICATION OF DOPAMINE ANTIBODY: Use a final dilution of 1:2,500-1:10,000 in Solution C containing 0.1% Triton X100 and 2% non-specific serum. Incubate 12 sections per 2 mL diluted antibody overnight, +4°C. Then wash the sections three times for 10 minutes each in Solution C. (Note that the antibody may be usable at a higher dilution. This should be explored to minimize the possibility of high background. Additionally, note that a change in the buffering system as indicated in the protocol may change the background and antibody recognition). The specific reaction is then revealed by PAP procedure.

6. SECOND ANTIBODY: Incubate the sections with a 1:50 to 1:200 dilution of goat anti-rabbit in Solution B containing 1% non-specific serum for either 3 hrs at 20°C or 2 hr at 37°C. Then wash the sections, 3 times, for 10 minutes each with Solution B.

7. PAP: Incubate the sections with the appropriate dilution of peroxidase anti-peroxidase (for free floating method) in Solution B containing 1% non-specific serum for 1-2 hours at 37°C. Then wash sections 3 times for 10 min each in solution B.

8. VISUALIZATION: The antigen-antibody complexes are visualized using DAB-4-HCl (25 mg/100 mL) (or other chromogen) in 0.05M Tris and filtrated; 0.05% hydrogen peroxide is added. Incubate the sections for 10 minutes at room temp. Stop the reaction by transferring the sections to 5 mL 0.05M Tris. Wash tissue with solution D using 2, 10 min washes. Mount sections on chrome-alum coated slides. Dry overnight at 37°C. Rehydrate sections using conventional histological procedures. Coverslip using rapid mounting media.

For research use only; not for use as a diagnostic.
Biological Information

The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:

Compound Cross-reactivity

Dopamine-G-BSA 1

L-DOPA-G-BSA 1/10,000

Tyrosine-G-BSA 1/36,000

Tyramine-G-BSA 1/>50,000

Noradrenaline-G-BSA 1/>50,000

Octopamine-G-BSA 1/>50,000

Adrenaline-G-BSA 1/>50,000

Dopamine 1/>50,000


(G) Glutaraldehyde

(BSA) Bovine Serum Albumin
Species Reactivity
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Global Trade Item Number
Catalogue Number GTIN
MAB5300 04053252504037


Anti-Dopamine Antibody, clone K56A SDS


Safety Data Sheet (SDS) 

Anti-Dopamine Antibody, clone K56A Certificates of Analysis

TitleLot Number
MOUSE ANTI-DOPAMINE - 3234562 3234562
MOUSE ANTI-DOPAMINE - 3317847 3317847
MOUSE ANTI-DOPAMINE - 3482892 3482892
MOUSE ANTI-DOPAMINE - 3674970 3674970
MOUSE ANTI-DOPAMINE -2548033 2548033
MOUSE ANTI-DOPAMINE -2580700 2580700
MOUSE ANTI-DOPAMINE -2640541 2640541
MOUSE ANTI-DOPAMINE -2762392 2762392
MOUSE ANTI-DOPAMINE -2829783 2829783


Reference overviewPub Med ID
Forebrain dopamine neurons project down to a brainstem region controlling locomotion.
Ryczko, D; Grätsch, S; Auclair, F; Dubé, C; Bergeron, S; Alpert, MH; Cone, JJ; Roitman, MF; Alford, S; Dubuc, R
Proceedings of the National Academy of Sciences of the United States of America  110  E3235-42  2013

Show Abstract
23918379 23918379
Disruption of dopamine homeostasis underlies selective neurodegeneration mediated by alpha-synuclein.
Soon S Park,Emily M Schulz,Daewoo Lee
The European journal of neuroscience  26  2007

Show Abstract
18005066 18005066

Data Sheet