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AB1536 Anti-Dopamine β Hydroxylase Antibody, CT

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AB1536
100 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Mk, R, RbIHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB1536
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Dopamine β Hydroxylase Antibody, CT
Alternate Names
  • DBH
References
Product Information
FormatAffinity Purified
PresentationLiquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-Dopamine β Hydroxylase Antibody, C-terminus is an antibody against Dopamine β Hydroxylase for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunoblotting: 1:250-1:1,000.

Immunohistochemistry: 1:250-1:1,000, staining was evaluated in the locus coeruleus of formaldehyde-fixed, cryopreserved human and monkey brain. Showed moderate staining on both human and monkey.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide, CPTSQGRSPAGPTVVSI-amide, cloned from human (DbH) cDNA (Nagatsu et al. 1990).
EpitopeC-terminus
HostRabbit
SpecificityDopamine Beta Hydroxylase (DbetaH) C-terminal. By immunoblot the antibody specifically reacts with a single (or double) band(s) of M.W. »70-75,000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE.
Species Reactivity
  • Human
  • Monkey
  • Rat
  • Rabbit
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide.
Gene Symbol
  • DBH
  • DBM
  • EC 1.14.17.1 [Contains: Soluble dopamine beta-hydroxylase].
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P09172 # Conversion of dopamine to noradrenaline.
COFACTOR: Binds 1 PQQ per subunit. & Binds 2 copper ions per subunit.
SIZE: 617 amino acids; 69065 Da
SUBUNIT: Homotetramer composed of two non-covalently bound disulfide-linked dimers.
SUBCELLULAR LOCATION: Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. & Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single- pass type II membrane protein (Potential).
DISEASE: SwissProt: P09172 # Defects in DBH are the cause of DBH deficiency [MIM:223360]; also called norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
SIMILARITY: SwissProt: P09172 ## Belongs to the copper type II ascorbate-dependent monooxygenase family. & Contains 1 DOMON domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze-thaw cycles.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB1536 04053252329531

Documentation

Anti-Dopamine β Hydroxylase Antibody, CT SDS

Title

Safety Data Sheet (SDS) 

Anti-Dopamine β Hydroxylase Antibody, CT Certificates of Analysis

TitleLot Number
RABBIT ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2398839 2398839
RABBIT ANTI-DOPAMINE BETA HYDROXYLASE -2775060 2775060
RABBIT ANTI-DOPAMINE BETA HYDROXYLASE -2781855 2781855
RABBIT ANTI-DOPAMINE BETA HYDROXYLASE -2809962 2809962
RABBIT ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL - 3290445 3290445
RABBIT ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL - 3537655 3537655
RABBIT ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL - 3863405 3863405
RABBIT ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL - 4162723 4162723
RABBIT ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL AFFINITY PURIFIED, POLYCLONAL ANTIBODY 2944643
RABBIT ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL AFFINITY PURIFIED, POLYCLONAL ANTIBODY 2986606

References

Reference overviewPub Med ID
Hypoxic preconditioning of human mesenchymal stem cells overcomes hypoxia-induced inhibition of osteogenic differentiation.
Elias Volkmer,Bobby Cherian Kallukalam,Josef Maertz,Sven Otto,Inga Drosse,Hans Polzer,Wolfgang Bocker,Michael Stengele,Denitsa Docheva,Wolf Mutschler,Matthias Schieker
Tissue engineering. Part A  16  2010

Show Abstract
19642854 19642854
Involvement of Hif-1 in desferrioxamine-induced invasion of glioblastoma cells.
Anja Elstner,Nikola Holtkamp,Andreas von Deimling
Clinical & experimental metastasis  24  2007

Show Abstract
17357815 17357815
Differentiation of human embryonic stem cells to dopaminergic neurons in serum-free suspension culture.
Thomas C Schulz, Scott A Noggle, Gail M Palmarini, Deb A Weiler, Ian G Lyons, Kate A Pensa, Adrian C B Meedeniya, Bruce P Davidson, Nevin A Lambert, Brian G Condie
Stem cells (Dayton, Ohio)  22  1218-38  2004

Show Abstract
15579641 15579641
The primary structure of human dopamine-beta-hydroxylase: insights into the relationship between the soluble and the membrane-bound forms of the enzyme
Lamouroux, A, et al
EMBO J, 6:3931-7 (1987)  1987

3443096 3443096

Data Sheet

Title
RABBIT ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL

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Product Families

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies