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MAB308 Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2

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100 µL  
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Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, R, ShIHC, IH(P), IFMAscitesMonoclonal Antibody
Catalogue NumberMAB308
ReplacesMAB394-250UG; MAB394-100UG; MAB394-25UG
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Dopamine β Hydroxylase Antibody, clone 4F10.2
Alternate Names
  • DBH
Background InformationDopamine Beta Hydroxylase (DBH), the structural locus encoding DβH enzyme, is a major quantitative trait locus influencing plasma DβH activity, with one single nucleotide polymorphism (SNP) accounting for up to 50% of the variance. Mutations at DBH appear to be responsible for the rare syndrome of DβH deficiency. It functions as the only enzyme involved in the synthesis of small-molecule neurotransmitters that is membrane-bound, making norepinephrine and epinephrine the only transmitters synthesized inside vesicles. It is expressed in noradrenergic nerve terminals of the central and peripheral nervous systems, as well as in chromaffin cells of the adrenal medulla.
Product Information
HS Code3002 15 90
  • Brain (Striatum/Substantia Nigra)
PresentationAscites mouse monoclonal IgG1 in liquid with 0.1% sodium azide.
Quality LevelMQ100
ApplicationAnti-Dopamine β Hydroxylase Antibody, clone 4F10.2 detects level of Dopamine β Hydroxylase & has been published & validated for use in IH, IH(P) & IF.
Key Applications
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
Application NotesIndirect immunofluorescence:
1:300-1:500 dilution of a previous lot was used on rat brain tissue.

Optimal working dilutions must be determined by the end user.

Representative images from a previous lot.

Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord
Biological Information
ImmunogenPurified bovine DBH.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
SpecificityDopamine beta hydroxylase (DBH).
Species Reactivity
  • Bovine
  • Rat
  • Sheep
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide.
Gene Symbol
  • DBH
  • DBM
  • EC [Contains: Soluble dopamine beta-hydroxylase].
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P09172 # Conversion of dopamine to noradrenaline.
COFACTOR: Binds 1 PQQ per subunit. & Binds 2 copper ions per subunit.
SIZE: 617 amino acids; 69065 Da
SUBUNIT: Homotetramer composed of two non-covalently bound disulfide-linked dimers.
SUBCELLULAR LOCATION: Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. & Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single- pass type II membrane protein (Potential).
DISEASE: SwissProt: P09172 # Defects in DBH are the cause of DBH deficiency [MIM:223360]; also called norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
SIMILARITY: SwissProt: P09172 ## Belongs to the copper type II ascorbate-dependent monooxygenase family. & Contains 1 DOMON domain.
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceImmunohistochemistry(paraffin): Dopamine β Hydroxylase (cat. # MAB308) staining pattern/morphology in rat spinal cord. Tissue was pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:300, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen predominantly expressed in the dorsal root ganglia (DRG).

Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Global Trade Item Number
Catalogue Number GTIN
MAB308 04053252577741


Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2 SDS


Safety Data Sheet (SDS) 

Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2 Certificates of Analysis

TitleLot Number
Anti-Dopamine Hydroxylase, clone 4F10.2 - 2102010 2102010
Anti-Dopamine Hydroxylase, clone 4F10.2 - LV1635956 LV1635956
Anti-Dopamine Hydroxylase, clone 4F10.2 - LV1696521 LV1696521
Anti-Dopamine Hydroxylase, clone 4F10.2 - LV1783152 LV1783152
Anti-Dopamine Hydroxylase, clone 4F10.2 - NG1873299 NG1873299
Anti-Dopamine #946; Hydroxylase, clone 4F10.2 - 2165839 2165839
Anti-Dopamine #946; Hydroxylase, clone 4F10.2 - 2029625 2029625
Anti-Dopamine Beta Hydroxylase, clone 4F10.2 - LV1556118 LV1556118
Anti-Dopamine β Hydroxylase, clone 4F10.2 - 2387531 2387531
Anti-Dopamine β Hydroxylase, clone 4F10.2 - 2464561 2464561


Reference overviewApplicationSpeciesPub Med ID
Lactate-mediated glia-neuronal signalling in the mammalian brain.
Tang, F; Lane, S; Korsak, A; Paton, JF; Gourine, AV; Kasparov, S; Teschemacher, AG
Nature communications  5  3284  2014

Show Abstract
24518663 24518663
Systemic leptin dose-dependently increases STAT3 phosphorylation within hypothalamic and hindbrain nuclei.
Maniscalco, JW; Rinaman, L
American journal of physiology. Regulatory, integrative and comparative physiology  306  R576-85  2014

Show Abstract
24523344 24523344
The rate of fall of blood glucose determines the necessity of forebrain-projecting catecholaminergic neurons for male rat sympathoadrenal responses.
Jokiaho, AJ; Donovan, CM; Watts, AG
Diabetes  63  2854-65  2014

Show Abstract
24740574 24740574
Optoactivation of locus ceruleus neurons evokes bidirectional changes in thermal nociception in rats.
Hickey, L; Li, Y; Fyson, SJ; Watson, TC; Perrins, R; Hewinson, J; Teschemacher, AG; Furue, H; Lumb, BM; Pickering, AE
The Journal of neuroscience : the official journal of the Society for Neuroscience  34  4148-60  2014

Show Abstract
24647936 24647936
Differential activation of chemically identified neurons in the caudal nucleus of the solitary tract in non-entrained rats after intake of satiating vs. non-satiating meals.
Kreisler, AD; Davis, EA; Rinaman, L
Physiology & behavior  136  47-54  2014

Show Abstract
24508750 24508750
A discrete dopaminergic projection from the incertohypothalamic A13 cell group to the dorsolateral periaqueductal gray in rat.
Messanvi, F; Eggens-Meijer, E; Roozendaal, B; van der Want, JJ
Frontiers in neuroanatomy  7  41  2013

Show Abstract
24367297 24367297
Astrocytes in the rat nucleus tractus solitarii are critical for cardiovascular reflex control.
Lin, LH; Moore, SA; Jones, SY; McGlashon, J; Talman, WT
The Journal of neuroscience : the official journal of the Society for Neuroscience  33  18608-17  2013

Show Abstract
ImmunohistochemistryRat24259582 24259582
Reduced c-Fos expression in medullary catecholaminergic neurons in rats 20 h after exposure to chronic intermittent hypoxia.
Herr, KB; Stettner, GM; Kubin, L
American journal of physiology. Regulatory, integrative and comparative physiology  304  R514-22  2013

Show Abstract
23364524 23364524
The puerperium alters spinal cord plasticity following peripheral nerve injury.
Gutierrez, S; Hayashida, K; Eisenach, JC
Neuroscience  228  301-8  2013

Show Abstract
23103215 23103215
Endogenous analgesic action of the pontospinal noradrenergic system spatially restricts and temporally delays the progression of neuropathic pain following tibial nerve injury.
Hughes, SW; Hickey, L; Hulse, RP; Lumb, BM; Pickering, AE
Pain  154  1680-90  2013

Show Abstract
23707289 23707289

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Product Families


Life Science Research > Antibodies and Assays > Primary Antibodies