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AB1591P Anti-Dopamine Transporter Antibody

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50 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RELISA, IHC, IP, WBRbAffinity PurifiedPolyclonal Antibody
Catalogue NumberAB1591P
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Dopamine Transporter Antibody
Alternate Names
  • DAT
Background InformationPlasmalemmal neurotransmitter transporters sequester synaptic and peri synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration. The activity of the DAT reuptake carrier is sodium dependent, and it is suspected to play a role in such neurologic and psychiatric disorders as Parkinson's disease, Tourette's disease, schizophrenia, and addiction. It is a 12 transmembrane domain transporter with the N and C terminal regions located within the cytoplasm.
Product Information
FormatAffinity Purified
HS Code3002 15 90
  • Rat brain tissue
PresentationAffinity Purified immunoglobulin. Liquid in PBS containing 0.1% BSA
Quality LevelMQ100
ApplicationDetect Dopamine Transporter using this Anti-Dopamine Transporter Antibody validated for use in ELISA, IH, IP & WB.
Key Applications
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern blot: 1-10 μg/mL using ECL. An antibody raised against a similar peptide has been shown to react with a broad 75-80 kDa band in rat striatum (Hastrup et al., 2001) and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55 kDa.

Immunoprecipitation: 2-10 μg of antibody (Chang et al., 2001)

Immunohistochemistry: 1:250-1:500 on frozen sections of tissues fixed with 4% paraformaldehyde (Chang et al., 2001; Ourednik, et al., 2002). 0.05% trypsin for 10-15min has been found effective in exposing the epitope in some preparations.

ELISA: 0.5-1.0 μg/mL using 1 μg/mL of DAT control peptide (Catalog Number AG296) per well.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenAn 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (Kilty et al. 1991; Giros et al. 1991; Shimada et al. 1991; Kitayma 1992). This region is presumably the cytoplasmic domain.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
SpecificityRecognizes Dopamine transporter (DAT). By Western blot the antibody recognizes a broad 80 kDa band in rat striatum (Lew et al. 1992; Freed et al. 1995; Vaughan et al. 1996) and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55 kDa.
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe dopamine transporter (DAT1) mediates the active reuptake of dopamine from the synapse and is a principal regulator of dopaminergic neurotransmission. The DAT1 gene has been implicated in human disorders such as parkinsonism, Tourette syndrome, and substance abuse (Vandenbergh et al., 1992 [PubMed 1359373]).[supplied by OMIM]
Gene Symbol
  • SLC6A3
  • ADHD
  • DAT
  • DAT1
  • ADD
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q01959 # Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
SIZE: 620 amino acids; 68495 Da
SUBUNIT: Homooligomer; disulfide-linked. Interacts with PRKCABP and TGFB1I1.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: Q01959 ## Belongs to the sodium:neurotransmitter symporter (SNF) family.
MISCELLANEOUS: This protein is the target of psychomotor stimulants such as amphetamines or cocaine.
Molecular Weight80 kDa
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Global Trade Item Number
Catalogue Number GTIN
AB1591P 04053252515811


Anti-Dopamine Transporter Antibody SDS


Safety Data Sheet (SDS) 

Anti-Dopamine Transporter Antibody Certificates of Analysis

TitleLot Number
Anti-Dopamine Transporter 2459023
Anti-Dopamine Transporter - 2148218 2148218
Anti-Dopamine Transporter - 2383064 2383064
Anti-Dopamine Transporter - 2446695 2446695
Anti-Dopamine Transporter - 2006903 2006903
Anti-Dopamine Transporter - 2008612 2008612
Anti-Dopamine Transporter - 2021180 2021180
Anti-Dopamine Transporter - 2067221 2067221
Anti-Dopamine Transporter - 2293859 2293859
Anti-Dopamine Transporter - 2519289 2519289


Reference overviewApplicationSpeciesPub Med ID
Striatal astrocytes act as a reservoir for L-DOPA.
Asanuma, M; Miyazaki, I; Murakami, S; Diaz-Corrales, FJ; Ogawa, N
PloS one  9  e106362  2014

Show Abstract
Immunohistochemistry25188235 25188235
Alteration of striatal dopaminergic neurotransmission in a mouse model of DYT11 myoclonus-dystonia.
Zhang, L; Yokoi, F; Parsons, DS; Standaert, DG; Li, Y
PloS one  7  e33669  2012

Show Abstract
22438980 22438980
Prenatal LPS increases inflammation in the substantia nigra of Gdnf heterozygous mice.
Granholm, AC; Zaman, V; Godbee, J; Smith, M; Ramadan, R; Umphlet, C; Randall, P; Bhat, NR; Rohrer, B; Middaugh, LD; Boger, HA
Brain pathology (Zurich, Switzerland)  21  330-48  2011

Show Abstract
20969653 20969653
Dopamine transporter glycosylation correlates with the vulnerability of midbrain dopaminergic cells in Parkinson's disease.
Domingo Afonso-Oramas,Ignacio Cruz-Muros,Diego Alvarez de la Rosa,Pedro Abreu,Teresa Giráldez,Javier Castro-Hernández,Josmar Salas-Hernández,José L Lanciego,Manuel Rodríguez,Tomas González-Hernández
Neurobiology of disease  36  2009

Show Abstract
19766189 19766189
The role of gamma-synuclein in cocaine-induced behaviour in rats.
Frederic Boyer, Jean-Luc Dreyer, Frederic Boyer, Jean-Luc Dreyer, Frederic Boyer, Jean-Luc Dreyer
The European journal of neuroscience  27  2938-51  2008

Show Abstract
18588534 18588534
Expression of the dopamine transporter in rat and bullfrog retinas.
Zhe Cheng, Yong-Mei Zhong, Xiong-Li Yang
Neuroreport  17  773-7  2006

Show Abstract
16708013 16708013
Repeated maternal separation: differences in cocaine-induced behavioral sensitization in adult male and female mice.
Takefumi Kikusui, Sara Faccidomo, Klaus A Miczek
Psychopharmacology  178  202-10  2005

Show Abstract
15322726 15322726
1-methyl-4-phenylpyridinium (MPP+) decreases mitochondrial oxidation-reduction (REDOX) activity and membrane potential (Deltapsi(m)) in rat striatum.
Masami Nakai, Atsushi Mori, Akihito Watanabe, Yasuhide Mitsumoto
Experimental neurology  179  103-10  2003

Show Abstract
12504872 12504872
Attenuation of dopamine transporter activity by alpha-synuclein.
Christophe Wersinger, Anita Sidhu, Christophe Wersinger, Anita Sidhu
Neuroscience letters  340  189-92  2003

Show Abstract
12672538 12672538
Effects of estradiol, phytoestrogens, and Ginkgo biloba extracts against 1-methyl-4-phenyl-pyridine-induced oxidative stress.
Benoit Gagné, Sylvie Gélinas, Geneviève Bureau, Bruno Lagacé, Charles Ramassamy, Keith Chiasson, Barbara Valastro, Maria-Grazia Martinoli
Endocrine  21  89-95  2003

Show Abstract
12777708 12777708

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Product Families


Life Science Research > Antibodies and Assays > Primary Antibodies