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MAB377 Anti-NeuN Antibody, clone A60

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500 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Av, Ch, Ft, H, M, Po, R, SalFC, ICC, IF, IHC, IH(P), IP, WBMPurifiedMonoclonal Antibody
Catalogue NumberMAB377
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-NeuN Antibody, clone A60
Alternate Names
  • Neuron-Specific Nuclear Protein
  • Neuna60
  • A60
Background InformationNeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).
Product Information
HS Code3002 15 90
  • Positive control -Brain Tissue. Negative control - Any non neuronal tissue eg Fibroblasts
PresentationPurified mouse immunoglobulin IgG1 liquid in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Quality LevelMQ100
ApplicationAnti-NeuN Antibody, clone A60 detects level of NeuN and has been published and validated for use in FC, IC, IF, IH, IH(P), IP and WB.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blot Analysis:
A previous lot of this antibody recognized 2-3 bands in the 46-48 kDa range and possibly another band at approximately 66 kDa.

1:10-1:100 dilution from a previous lot was used. Neurons in culture should be permeablized with 0.1% triton X-100. All primary antibody dilutions should be performed with simple solutions containing only buffer and primary antibody without excess protein blocks or detergents.

1:100-1:1,000. The antibody works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. The antibody works well with formaldehyde-based fixatives. Citric acid and microwave pretreatment has been used successfully (Sarnat, 1998).

Immunohistochemistry(paraffin) Analysis: A previous lot was used for IH(P).

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified cell nuclei from mouse brain
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
SpecificityMILLIPORE's exclusive monoclonal antibody to vertebrate neuron-specific nuclear protein called NeuN (or Neuronal Nuclei) reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive with MAB377 include Purkinje, mitral and photoreceptor cells. The antibody is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants.
Species Reactivity
  • Avian
  • Chicken
  • Ferret
  • Human
  • Mouse
  • Pig
  • Rat
  • Salamander
Antibody TypeMonoclonal Antibody
Gene Symbol
  • NeuN
  • A58
Purification MethodProtein A Purfied
Molecular Weight46/48 kDa
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunohistochemistry on brain tissue.

Immunohistochemistry(paraffin) Analysis:
NeuN (cat. # MAB377) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as nuclear staining in the neurons in the granular layer. Note that there is no signal detected in the nucleus of Purkinje cells.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at 2-8ºC from date of receipt.
Packaging Information
Material Size500 µg
Transport Information
Supplemental Information
Global Trade Item Number
Catalogue Number GTIN
MAB377 08436037123641


Anti-NeuN Antibody, clone A60 SDS


Safety Data Sheet (SDS) 

Anti-NeuN Antibody, clone A60 Certificates of Analysis

TitleLot Number
Anti-NeuN, clone A60 3075598
Anti-NeuN, clone A60 - 2118033 2118033
Anti-NeuN, clone A60 - 2375608 2375608
Anti-NeuN, clone A60 - 2392283 2392283
Anti-NeuN, clone A60 - 2424507 2424507
Anti-NeuN, clone A60 - 2428671 2428671
Anti-NeuN, clone A60 - 2453249 2453249
Anti-NeuN, clone A60 - 1991263 1991263
Anti-NeuN, clone A60 - 2062313 2062313
Anti-NeuN, clone A60 - 2074765 2074765


Reference overviewApplicationSpeciesPub Med ID
Clostridium perfringens Epsilon Toxin Causes Selective Death of Mature Oligodendrocytes and Central Nervous System Demyelination.
Linden, JR; Ma, Y; Zhao, B; Harris, JM; Rumah, KR; Schaeren-Wiemers, N; Vartanian, T
mBio  6  e02513  2015

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26081637 26081637
When larger brains do not have more neurons: increased numbers of cells are compensated by decreased average cell size across mouse individuals.
Herculano-Houzel, S; Messeder, DJ; Fonseca-Azevedo, K; Pantoja, NA
Frontiers in neuroanatomy  9  64  2015

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26082686 26082686
Somatic CRISPR/Cas9-mediated tumour suppressor disruption enables versatile brain tumour modelling.
Zuckermann, M; Hovestadt, V; Knobbe-Thomsen, CB; Zapatka, M; Northcott, PA; Schramm, K; Belic, J; Jones, DT; Tschida, B; Moriarity, B; Largaespada, D; Roussel, MF; Korshunov, A; Reifenberger, G; Pfister, SM; Lichter, P; Kawauchi, D; Gronych, J
Nature communications  6  7391  2015

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26067104 26067104
Expression changes of microRNA-1 and its targets Connexin 43 and brain-derived neurotrophic factor in the peripheral nervous system of chronic neuropathic rats.
Neumann, E; Hermanns, H; Barthel, F; Werdehausen, R; Brandenburger, T
Molecular pain  11  39  2015

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26111928 26111928
Targeted Overexpression of α-Synuclein by rAAV2/1 Vectors Induces Progressive Nigrostriatal Degeneration and Increases Vulnerability to MPTP in Mouse.
Song, LK; Ma, KL; Yuan, YH; Mu, Z; Song, XY; Niu, F; Han, N; Chen, NH
PloS one  10  e0131281  2015

Show Abstract
26114655 26114655
Pre-existing astrocytes form functional perisynaptic processes on neurons generated in the adult hippocampus.
Krzisch, M; Temprana, SG; Mongiat, LA; Armida, J; Schmutz, V; Virtanen, MA; Kocher-Braissant, J; Kraftsik, R; Vutskits, L; Conzelmann, KK; Bergami, M; Gage, FH; Schinder, AF; Toni, N
Brain structure & function  220  2027-42  2015

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24748560 24748560
Nicotine accelerates diabetes-induced retinal changes.
Boretsky, A; Gupta, P; Tirgan, N; Liu, R; Godley, BF; Zhang, W; Tilton, RG; Motamedi, M
Current eye research  40  368-77  2015

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24911405 24911405
An anterograde rabies virus vector for high-resolution large-scale reconstruction of 3D neuron morphology.
Haberl, MG; Viana da Silva, S; Guest, JM; Ginger, M; Ghanem, A; Mulle, C; Oberlaender, M; Conzelmann, KK; Frick, A
Brain structure & function  220  1369-79  2015

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24723034 24723034
Enrichment rescues contextual discrimination deficit associated with immediate shock.
Clemenson, GD; Lee, SW; Deng, W; Barrera, VR; Iwamoto, KS; Fanselow, MS; Gage, FH
Hippocampus  25  385-92  2015

Show Abstract
25330953 25330953
The expression of Mas-receptor of the renin-angiotensin system in the human eye.
Vaajanen, A; Kalesnykas, G; Vapaatalo, H; Uusitalo, H
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie  253  1053-9  2015

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Alternative Format

Catalogue Number Description
MAB377B Anti-NeuN Antibody, clone A60, biotin conjugated
MAB377X Anti-NeuN Antibody, clone A60, Alexa Fluor®488 conjugated

Product Families


Life Science Research > Antibodies and Assays > Primary Antibodies