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AB152 Anti-Tyrosine Hydroxylase Antibody

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AB152
100 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, M, Fe, Ft, Sqd, Dr, MlELISA, IF, IHC, IH(P), IP, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB152
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Tyrosine Hydroxylase Antibody
Alternate Names
  • Tyrosine 3-hydroxylase
  • Tyrosine 3-monooxygenase
  • tyrosine hydroxylase
  • TH
Background InformationTyrosine hydroxylase (also know as Tyrosine 3-hydroxylase and Tyrosine 3-monooxygenase) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
Control
  • POSITIVE CONTROL: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands.
    NEGATATIVE CONTROL: Liver.
PresentationPurified rabbit polyclonal in buffer containing liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl (pH 7.5), 100 ug/mL BSA and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-Tyrosine Hydroxylase Antibody detects level of TH and has been published and validated for use in ELISA, IF, IH, IH(P), IP and WB.
Key Applications
  • ELISA
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry:
A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.

Immunofluorescence:
A previous lot of this antibody was used at 1:100-1:1,000 dilution.

Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.

ELISA:
A previous lot of this antibody was used in ELISA.

Optimal dilutions must be determined by the end user.
Biological Information
ImmunogenDenatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).
HostRabbit
SpecificityTyrosine hydroxylase. By western blot, AB152 selective labels a single band at approximately 62kDa (reduced) corresponding to Tyrosine Hydroxylase. It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
Species Reactivity
  • Human
  • Rat
  • Mouse
  • Feline
  • Ferret
  • Squid
  • Drosophila
  • Mollusk
Species Reactivity NoteRat, mouse, ferret, feline, Aplysia (marine mollusc). It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
Gene Symbol
  • TH
  • TYH
  • EC 1.14.16.2
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
COFACTOR: Fe(2+) ion.
SIZE: 528 amino acids; 58524 Da
TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Molecular Weight62 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain frozen at -20ºC to -70ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB152 04053252287138

Documentation

Anti-Tyrosine Hydroxylase Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Tyrosine Hydroxylase Antibody Certificates of Analysis

TitleLot Number
Anti-Tyrosine Hydroxylase 3072361
Anti-Tyrosine Hydroxylase - 2115918 2115918
Anti-Tyrosine Hydroxylase - 2388829 2388829
Anti-Tyrosine Hydroxylase - 2392225 2392225
Anti-Tyrosine Hydroxylase - 2426518 2426518
Anti-Tyrosine Hydroxylase - 2458991 2458991
Anti-Tyrosine Hydroxylase - 1951919 1951919
Anti-Tyrosine Hydroxylase - 1994651 1994651
Anti-Tyrosine Hydroxylase - 2021194 2021194
Anti-Tyrosine Hydroxylase - 2038257 2038257

References

Reference overviewApplicationSpeciesPub Med ID
Spatiotemporal expression and transcriptional perturbations by long noncoding RNAs in the mouse brain.
Goff, LA; Groff, AF; Sauvageau, M; Trayes-Gibson, Z; Sanchez-Gomez, DB; Morse, M; Martin, RD; Elcavage, LE; Liapis, SC; Gonzalez-Celeiro, M; Plana, O; Li, E; Gerhardinger, C; Tomassy, GS; Arlotta, P; Rinn, JL
Proceedings of the National Academy of Sciences of the United States of America  112  6855-62  2015

Show Abstract
26034286 26034286
Forebrain deletion of the dystonia protein torsinA causes dystonic-like movements and loss of striatal cholinergic neurons.
Pappas, SS; Darr, K; Holley, SM; Cepeda, C; Mabrouk, OS; Wong, JM; LeWitt, TM; Paudel, R; Houlden, H; Kennedy, RT; Levine, MS; Dauer, WT
eLife  4  e08352  2015

Show Abstract
26052670 26052670
Highly efficient direct conversion of human fibroblasts to neuronal cells by chemical compounds.
Dai, P; Harada, Y; Takamatsu, T
Journal of clinical biochemistry and nutrition  56  166-70  2015

Show Abstract
26060345 26060345
shRNA targeting α-synuclein prevents neurodegeneration in a Parkinson's disease model.
Zharikov, AD; Cannon, JR; Tapias, V; Bai, Q; Horowitz, MP; Shah, V; El Ayadi, A; Hastings, TG; Greenamyre, JT; Burton, EA
The Journal of clinical investigation  125  2721-35  2015

Show Abstract
26075822 26075822
Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B.
Batassini, C; Broetto, N; Tortorelli, LS; Borsoi, M; Zanotto, C; Galland, F; Souza, TM; Leite, MC; Gonçalves, CA
Neural plasticity  2015  387028  2015

Show Abstract
26090233 26090233
Nigral overexpression of alpha-synuclein in the absence of parkin enhances alpha-synuclein phosphorylation but does not modulate dopaminergic neurodegeneration.
Van Rompuy, AS; Oliveras-Salvá, M; Van der Perren, A; Corti, O; Van den Haute, C; Baekelandt, V
Molecular neurodegeneration  10  23  2015

Show Abstract
26099628 26099628
PGC-1α activity in nigral dopamine neurons determines vulnerability to α-synuclein.
Ciron, C; Zheng, L; Bobela, W; Knott, GW; Leone, TC; Kelly, DP; Schneider, BL
Acta neuropathologica communications  3  16  2015

Show Abstract
25853296 25853296
Small peptide inhibitor of JNK3 protects dopaminergic neurons from MPTP induced injury via inhibiting the ASK1-JNK3 signaling pathway.
Pan, J; Li, H; Zhang, B; Xiong, R; Zhang, Y; Kang, WY; Chen, W; Zhao, ZB; Chen, SD
PloS one  10  e0119204  2015

Show Abstract
25856433 25856433
Novel fast adapting interneurons mediate cholinergic-induced fast GABAA inhibitory postsynaptic currents in striatal spiny neurons.
Faust, TW; Assous, M; Shah, F; Tepper, JM; Koós, T
The European journal of neuroscience  42  1764-74  2015

Show Abstract
25865337 25865337
Conditional targeting of medium spiny neurons in the striatal matrix.
Reinius, B; Blunder, M; Brett, FM; Eriksson, A; Patra, K; Jonsson, J; Jazin, E; Kullander, K
Frontiers in behavioral neuroscience  9  71  2015

Show Abstract
25870547 25870547

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