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AB1542 Anti-Tyrosine Hydroxylase Antibody

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ma, R, MIHC, WBShAffinity PurifiedPolyclonal Antibody
Catalogue NumberAB1542
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Tyrosine Hydroxylase Antibody
Alternate Names
  • Tyrosine 3-hydroxylase
  • Tyrosine 3-hydroxylase
  • tyrosine hydroxylase
Background InformationTyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Product Information
FormatAffinity Purified
HS Code3002 15 90
  • Positive: brain (corpus striatum) and adrenal gland. Negative: liver.
PresentationPurified sheep polyclonal in buffer containing 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Quality LevelMQ100
ApplicationAnti-Tyrosine Hydroxylase Antibody is an antibody against Tyrosine Hydroxylase for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry(peroxidase): A previous lot of this antibody was used at 1:1,000 dilution. The antibody gives specific labeling of noradrenergic axons in primate cerebral cortex (Brain Res., 1989, 500:313-324.).
Note: It is important that you use a secondary antibody that has significant reactivity to sheep IgG1 since the majority of the sheep immunoglobulin is IgG1.
Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenNative tyrosine hydroxylase from rat pheochromocytoma (J. Biol. Chem.,1982, 257:9416-9423).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
SpecificityTyrosine Hydroxylase (TH, Tyrosine Monooxygenase). Cross-reacts with all mammalian forms tested to date and some non-mammalian forms.
Species Reactivity
  • Mammals
  • Rat
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq].

Transcript Variant: This variant (2) uses a different donor splice site at the first coding exon and is missing an adjacent in-frame coding exon compared to transcript variant 1, resulting in an isoform (b) missing a 31 aa segment compared to isoform a.
Gene Symbol
  • TH
  • TYH
  • EC
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
COFACTOR: Fe(2+) ion.
SIZE: 528 amino acids; 58524 Da
TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Molecular Weight~60 kDa
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of mouse brain lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at -20 to -80°C in undiluted aliquots from date of receipt. Store in a NON-self-defrosting freezer.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Global Trade Item Number
Catalogue Number GTIN
AB1542 04053252323706


Anti-Tyrosine Hydroxylase Antibody SDS


Safety Data Sheet (SDS) 

Anti-Tyrosine Hydroxylase Antibody Certificates of Analysis

TitleLot Number
Anti-Tyrosine Hydroxylase 3076453
Anti-Tyrosine Hydroxylase - 2115919 2115919
Anti-Tyrosine Hydroxylase - 2136987 2136987
Anti-Tyrosine Hydroxylase - 2386487 2386487
Anti-Tyrosine Hydroxylase - 2430338 2430338
Anti-Tyrosine Hydroxylase - 1970692 1970692
Anti-Tyrosine Hydroxylase - 2031806 2031806
Anti-Tyrosine Hydroxylase - 2188466 2188466
Anti-Tyrosine Hydroxylase - 2324511 2324511
Anti-Tyrosine Hydroxylase - 2519288 2519288


Reference overviewApplicationSpeciesPub Med ID
Norepinephrine activates dopamine D4 receptors in the rat lateral habenula.
Root, DH; Hoffman, AF; Good, CH; Zhang, S; Gigante, E; Lupica, CR; Morales, M
The Journal of neuroscience : the official journal of the Society for Neuroscience  35  3460-9  2015

Show Abstract
25716845 25716845
Coronin-1 and calcium signaling governs sympathetic final target innervation.
Suo, D; Park, J; Young, S; Makita, T; Deppmann, CD
The Journal of neuroscience : the official journal of the Society for Neuroscience  35  3893-902  2015

Show Abstract
25740518 25740518
Genome-wide characterisation of Foxa1 binding sites reveals several mechanisms for regulating neuronal differentiation in midbrain dopamine cells.
Metzakopian, E; Bouhali, K; Alvarez-Saavedra, M; Whitsett, JA; Picketts, DJ; Ang, SL
Development (Cambridge, England)  142  1315-24  2015

Show Abstract
25804738 25804738
Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons.
Zhang, S; Qi, J; Li, X; Wang, HL; Britt, JP; Hoffman, AF; Bonci, A; Lupica, CR; Morales, M
Nature neuroscience  18  386-92  2015

Show Abstract
25664911 25664911
Hypothalamic overexpression of mutant huntingtin causes dysregulation of brown adipose tissue.
Soylu-Kucharz, R; Adlesic, N; Baldo, B; Kirik, D; Petersén, Å
Scientific reports  5  14598  2015

Show Abstract
26419281 26419281
Sexual dimorphism in the hypophysiotropic tyrosine hydroxylase-positive neurons in the preoptic area of the teleost, Clarias batrachus.
Saha, S; Patil, S; Singh, U; Singh, O; Singru, PS
Biology of sex differences  6  23  2015

Show Abstract
26557978 26557978
Pomegranate juice exacerbates oxidative stress and nigrostriatal degeneration in Parkinson's disease.
Tapias, V; Cannon, JR; Greenamyre, JT
Neurobiology of aging  35  1162-76  2014

Show Abstract
24315037 24315037
Mesodiencephalic dopaminergic neuronal differentiation does not involve GLI2A-mediated SHH-signaling and is under the direct influence of canonical WNT signaling.
Mesman, S; von Oerthel, L; Smidt, MP
PloS one  9  e97926  2014

Show Abstract
24865218 24865218
Enhanced synthesis and release of dopamine in transgenic mice with gain-of-function α6* nAChRs.
Wang, Y; Lee, JW; Oh, G; Grady, SR; McIntosh, JM; Brunzell, DH; Cannon, JR; Drenan, RM
Journal of neurochemistry  129  315-27  2014

Show Abstract
24266758 24266758
2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is selectively toxic to primary dopaminergic neurons in vitro.
Griggs, AM; Agim, ZS; Mishra, VR; Tambe, MA; Director-Myska, AE; Turteltaub, KW; McCabe, GP; Rochet, JC; Cannon, JR
Toxicological sciences : an official journal of the Society of Toxicology  140  179-89  2014

Show Abstract
24718704 24718704

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