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MAB5280 Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15

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40 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, Ch, Qu, RIHC, WBMPurifiedMonoclonal Antibody
Catalogue NumberMAB5280
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Tyrosine Hydroxylase Antibody, clone 2/40/15
Alternate Names
  • TH
  • Tyrosine Monooxygenase
Background InformationTyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Product Information
HS Code3002 15 90
  • Positive Control: Brain (sympathetic nerve terminals) and adrenal gland tissue
PresentationPurified mouse monoclonal IgG2a in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Quality LevelMQ100
ApplicationThis Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15 is validated for use in IH, WB for the detection of Tyrosine Hydroxylase.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry:
1:100-1:500. A previous lot of this antibody was used in immunohistochemistry.

Western blot:

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified tyrosine hydroxylase (EC from a rat pheochromocytoma (2).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
SpecificityTyrosine hydroxylase (TH) catalyzes the rate-determining initial step in the biosynthesis of catecholamines such as dopamine, noradrenaline and adrenaline. Therefore the antibody is used to stain dopaminergeous and adrenergeous neurons as well as the chromaffine tissue of the adrenal medulla. Cell lines such as the PC 12 line, which is derived from a rat pheochromocytoma also, show positive reactions. In immunoblots of adrenal medulla tissue, the antibody recognizes a single protein band in the molecular weight range of 56 - 60 kD.
Species Reactivity
  • Bovine
  • Chicken
  • Quail
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
Gene Symbol
  • TH
  • TYH
  • EC
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
COFACTOR: Fe(2+) ion.
SIZE: 528 amino acids; 58524 Da
TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Molecular Weight56-60 kDa
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of PC12 lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Packaging Information
Material Size40 µg
Transport Information
Supplemental Information
Global Trade Item Number
Catalogue Number GTIN
MAB5280 04053252398896


Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15 SDS


Safety Data Sheet (SDS) 

Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15 Certificates of Analysis

TitleLot Number
Anti-Tyrosine Hydroxylase, clone -2710260 2710260
Anti-Tyrosine Hydroxylase, clone -2789803 2789803
Anti-Tyrosine Hydroxylase, clone 2/40/15 - 2430442 2430442
Anti-Tyrosine Hydroxylase, clone 2/40/15 - 1975074 1975074
Anti-Tyrosine Hydroxylase, clone 2/40/15 - 2006934 2006934
Anti-Tyrosine Hydroxylase, clone 2/40/15 - 2043908 2043908
Anti-Tyrosine Hydroxylase, clone 2/40/15 - 2204912 2204912
Anti-Tyrosine Hydroxylase, clone 2/40/15 - 2318788 2318788
Anti-Tyrosine Hydroxylase, clone 2/40/15 - 2491186 2491186
Anti-Tyrosine Hydroxylase, clone 2/40/15 - 2563992 2563992


Reference overviewApplicationSpeciesPub Med ID
Chronic nicotine activates stress/reward-related brain regions and facilitates the transition to compulsive alcohol drinking.
Leão, RM; Cruz, FC; Vendruscolo, LF; de Guglielmo, G; Logrip, ML; Planeta, CS; Hope, BT; Koob, GF; George, O
The Journal of neuroscience : the official journal of the Society for Neuroscience  35  6241-53  2015

Show Abstract
25878294 25878294
A role for nonapeptides and dopamine in nest-building behaviour.
Hall, ZJ; Healy, SD; Meddle, SL
Journal of neuroendocrinology  27  158-65  2015

Show Abstract
25514990 25514990
Patterns of Brain Activation and Meal Reduction Induced by Abdominal Surgery in Mice and Modulation by Rikkunshito.
Wang, L; Mogami, S; Yakabi, S; Karasawa, H; Yamada, C; Yakabi, K; Hattori, T; Taché, Y
PloS one  10  e0139325  2015

Show Abstract
26421719 26421719
Ultrastructural localization of tyrosine hydroxylase in tree shrew nucleus accumbens core and shell.
McCollum, LA; Roberts, RC
Neuroscience  271  23-34  2014

Show Abstract
24769226 24769226
A population of glomerular glutamatergic neurons controls sensory information transfer in the mouse olfactory bulb.
Tatti, R; Bhaukaurally, K; Gschwend, O; Seal, RP; Edwards, RH; Rodriguez, I; Carleton, A
Nature communications  5  3791  2014

Show Abstract
24804702 24804702
Development of attenuated baroreflexes in obese Zucker rats coincides with impaired activation of nucleus tractus solitarius.
Guimaraes, PS; Huber, DA; Campagnole-Santos, MJ; Schreihofer, AM
American journal of physiology. Regulatory, integrative and comparative physiology  306  R681-92  2014

Show Abstract
24573182 24573182
Reelin demarcates a subset of pre-Bötzinger complex neurons in adult rat.
Tan, W; Sherman, D; Turesson, J; Shao, XM; Janczewski, WA; Feldman, JL
The Journal of comparative neurology  520  606-19  2012

Show Abstract
21858819 21858819
Stress stimulates production of catecholamines in rat adipocytes.
R Kvetnansky,J Ukropec,M Laukova,B Manz,K Pacak,P Vargovic
Cellular and molecular neurobiology  32  2012

Show Abstract
22402834 22402834
Disease-specific phenotypes in dopamine neurons from human iPS-based models of genetic and sporadic Parkinson's disease.
Sánchez-Danés, A; Richaud-Patin, Y; Carballo-Carbajal, I; Jiménez-Delgado, S; Caig, C; Mora, S; Di Guglielmo, C; Ezquerra, M; Patel, B; Giralt, A; Canals, JM; Memo, M; Alberch, J; López-Barneo, J; Vila, M; Cuervo, AM; Tolosa, E; Consiglio, A; Raya, A
EMBO molecular medicine  4  380-95  2012

Show Abstract
ImmunofluorescenceHuman22407749 22407749
Dopamine pathology in schizophrenia: analysis of total and phosphorylated tyrosine hydroxylase in the substantia nigra.
Perez-Costas, E; Melendez-Ferro, M; Rice, MW; Conley, RR; Roberts, RC
Frontiers in psychiatry  3  31  2012

Show Abstract
22509170 22509170

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Life Science Research > Antibodies and Assays > Primary Antibodies