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MAB318 Anti-Tyrosine Hydroxylase Antibody, clone LNC1

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MAB318
100 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ch, Fg, H, M, Mk, R, Lz, Vo, ZebrafishIHC, IH(P), IP, WBMAscitesMonoclonal Antibody
Description
Catalogue NumberMAB318
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Tyrosine Hydroxylase Antibody, clone LNC1
Alternate Names
  • TH
  • Tyrosine Monooxygenase
Background InformationTyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
References
Product Information
FormatAscites
HS Code3002 15 90
Control
  • Human brain tissue, extracts from 3T3 cells treated with Forskolin (40 nM, 30 min).
PresentationAscites mouse monoclonal IgG1κ fluid containing 3% BSA. Contains no preservative.
Quality LevelMQ100
Applications
ApplicationDetect Tyrosine Hydroxylase using this Anti-Tyrosine Hydroxylase Antibody, clone LNC1 validated for use in IH, IH(P), IP & WB with more than 85 product citations.
Key Applications
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry(paraffin):
A 1:200-1:400 dilution of a previous lot was used in IH. 4% PFA fixed, frozen sections; 4% PFA fixed, paraffin sections 1:100 (Barrachina, M. et al., 2003). For paraffin sections Barrachina reported successful staining with microwave citrate acid antigen recovery, however other methods can likely be used as well.

Immunoprecipitation:
A previous lot of this antibody was used in IP (Perez, 2002).

Optimal working dilutions and protocols must be determined by end user.
Biological Information
ImmunogenTyrosine Hydroxylase purified from PC12 cells
CloneLNC1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes an epitope on the outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT).
IsotypeIgG1κ
Species Reactivity
  • Chicken
  • Frog
  • Human
  • Mouse
  • Monkey
  • Rat
  • Lizard
  • Vole
  • Zebrafish
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
Gene Symbol
  • TH
  • TYH
  • EC 1.14.16.2
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
COFACTOR: Fe(2+) ion.
SIZE: 528 amino acids; 58524 Da
TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Molecular Weight59-63 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

Western blot:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of Mouse Brain lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB318 08436037123320

Documentation

Anti-Tyrosine Hydroxylase Antibody, clone LNC1 SDS

Title

Safety Data Sheet (SDS) 

Anti-Tyrosine Hydroxylase Antibody, clone LNC1 Certificates of Analysis

TitleLot Number
Anti-Tyrosine Hydroxylase, clone LNC1 2464515
Anti-Tyrosine Hydroxylase, clone LNC1 - 2127781 2127781
Anti-Tyrosine Hydroxylase, clone LNC1 - 2366531 2366531
Anti-Tyrosine Hydroxylase, clone LNC1 - 2430129 2430129
Anti-Tyrosine Hydroxylase, clone LNC1 - 2003001 2003001
Anti-Tyrosine Hydroxylase, clone LNC1 - 2061430 2061430
Anti-Tyrosine Hydroxylase, clone LNC1 - 2211927 2211927
Anti-Tyrosine Hydroxylase, clone LNC1 - 2276308 2276308
Anti-Tyrosine Hydroxylase, clone LNC1 - 2499557 2499557
Anti-Tyrosine Hydroxylase, clone LNC1 - 2950738 2950738

References

Reference overviewApplicationSpeciesPub Med ID
shRNA targeting α-synuclein prevents neurodegeneration in a Parkinson's disease model.
Zharikov, AD; Cannon, JR; Tapias, V; Bai, Q; Horowitz, MP; Shah, V; El Ayadi, A; Hastings, TG; Greenamyre, JT; Burton, EA
The Journal of clinical investigation  125  2721-35  2015

Show Abstract
26075822 26075822
Nigral overexpression of alpha-synuclein in the absence of parkin enhances alpha-synuclein phosphorylation but does not modulate dopaminergic neurodegeneration.
Van Rompuy, AS; Oliveras-Salvá, M; Van der Perren, A; Corti, O; Van den Haute, C; Baekelandt, V
Molecular neurodegeneration  10  23  2015

Show Abstract
26099628 26099628
Catecholaminergic innervation of central and peripheral auditory circuitry varies with reproductive state in female midshipman fish, Porichthys notatus.
Forlano, PM; Ghahramani, ZN; Monestime, CM; Kurochkin, P; Chernenko, A; Milkis, D
PloS one  10  e0121914  2015

Show Abstract
25849450 25849450
Norepinephrine activates dopamine D4 receptors in the rat lateral habenula.
Root, DH; Hoffman, AF; Good, CH; Zhang, S; Gigante, E; Lupica, CR; Morales, M
The Journal of neuroscience : the official journal of the Society for Neuroscience  35  3460-9  2015

Show Abstract
25716845 25716845
The temporal expression pattern of alpha-synuclein modulates olfactory neurogenesis in transgenic mice.
Schreglmann, SR; Regensburger, M; Rockenstein, E; Masliah, E; Xiang, W; Winkler, J; Winner, B
PloS one  10  e0126261  2015

Show Abstract
25961568 25961568
Viable neuronopathic Gaucher disease model in Medaka (Oryzias latipes) displays axonal accumulation of alpha-synuclein.
Uemura, N; Koike, M; Ansai, S; Kinoshita, M; Ishikawa-Fujiwara, T; Matsui, H; Naruse, K; Sakamoto, N; Uchiyama, Y; Todo, T; Takeda, S; Yamakado, H; Takahashi, R
PLoS genetics  11  e1005065  2015

Show Abstract
Immunohistochemistry25835295 25835295
Localization of reelin signaling pathway components in murine midbrain and striatum.
Sharaf, A; Rahhal, B; Spittau, B; Roussa, E
Cell and tissue research  359  393-407  2015

Show Abstract
Mouse25418135 25418135
A distinct subtype of dopaminergic interneuron displays inverted structural plasticity at the axon initial segment.
Chand, AN; Galliano, E; Chesters, RA; Grubb, MS
The Journal of neuroscience : the official journal of the Society for Neuroscience  35  1573-90  2015

Show Abstract
25632134 25632134
Dopaminergic and glutamatergic microdomains in a subset of rodent mesoaccumbens axons.
Zhang, S; Qi, J; Li, X; Wang, HL; Britt, JP; Hoffman, AF; Bonci, A; Lupica, CR; Morales, M
Nature neuroscience  18  386-92  2015

Show Abstract
25664911 25664911
Lack of additive role of ageing in nigrostriatal neurodegeneration triggered by α-synuclein overexpression.
Bourdenx, M; Dovero, S; Engeln, M; Bido, S; Bastide, MF; Dutheil, N; Vollenweider, I; Baud, L; Piron, C; Grouthier, V; Boraud, T; Porras, G; Li, Q; Baekelandt, V; Scheller, D; Michel, A; Fernagut, PO; Georges, F; Courtine, G; Bezard, E; Dehay, B
Acta neuropathologica communications  3  46  2015

Show Abstract
26205255 26205255

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Life Science Research > Antibodies and Assays > Primary Antibodies