ABE1976 Anti-XPC Antibody

ABE1976
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABE1976
      DescriptionAnti-XPC Antibody
      Alternate Names
      • DNA repair protein complementing XP-C cells
      • xeroderma pigmentosum group C-complementing protein
      • p125
      Background InformationDNA repair protein complementing XP-C cells (UniProt Q01831; also known as xeroderma pigmentosum group C-complementing protein, p125) is encoded by the XPC (also known as RAD4, XP3, XPCC) gene (Gene ID 7508) in human. Nucleotide excision repair (NER) removes damage to DNA caused by UV and mutagens. The NER process involves damage recognition and formation of an open DNA structure around the lesion, followed by dual incision, excision of an oligonucleotide of 24 to 32 residues, and gap-filling DNA synthesis to restore the DNA molecule. In human, the minimal set of components involved in NER includes replication protein A (RPA), XPA, XPC-RAD23B, XPG, ERCC1-XPF, TFIIH, replication factor C (RFC), PCNA, DNA polymerase δ or ɛ, and DNA ligase I. These factors can be divided into two groups, the six core factors necessary for damage recognition and dual incision (RPA, XPA, XPC-RAD23B complex, XPG, ERCC1-XPF, and TFIIH) and those for repair DNA synthesis in the gap-filling step. XPC is specifically involved in global genome NER (GG-NER) and serves as a damage recognition protein in complex with RAD23B. XPC is the rate-limiting factor in the GG-NER process, and XPC-dependent distortion recognition plays an essential role in initiating the recruitment of core NER proteins to the lesion.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-XPC Antibody is validated for use in Western Blotting for the detection of XPC.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected XPC in CBP-HMGB1 affinity pull-downs from XP12RO human xeroderma pigmentosum cell lysates (Lange, S.S., et al. (2009). DNA Repair (Amst). 8(7):865-872).
      Western Blotting Analysis: A representative lot detected XPC as one of the nucleotide excision repair (NER) proteins present in the immunoprecipitated TFIIH complex from HeLa cell lysates (Araújo, S.J., et al. (2001). Mol. Cell Biol. 21(7):2281-2291).
      Biological Information
      ImmunogenRecombinant fragment corresponding to human XPC amino acids 96-299.
      EpitopeAmino acids 96-299.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityExpected to react with all three spliced isoforms reported by UniProt (Q01831). This polyclonal antibody is known as RW028 in publications.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RAD4
      • XP3
      • XPC
      • XPCC
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~125 kDa observed. 106.0 kDa (isoform 1) 101.8 kDa (isoform 2) and 15.71 kDa (isoform 3) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in LNCaP nuclear extract.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected XPC in 10 µg of LNCaP nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-XPC Antibody Certificates of Analysis

      TitleLot Number
      Anti-XPC -Q2642754Q2642754