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71546 BacMagic™ Transfection Kit - Novagen

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Catalogue NumberPackaging Qty/Pack
71546-3 Plastic ampoule 5 rxn
OverviewThe BacMagic™-1 Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. BacMagic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac™, pBAC™, or pTriEx™ vectors, is cotransfected with BacMagic DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The BacMagic™-1 Transfection Kit contains the BacMagic™-1 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. In addition, it contains Sf9 cells and BacVector® Medium, for optimal transfection performance.
Catalogue Number71546
Brand Family Novagen®
Application Data
Figure 1. Construction of baculovirus recombinants with three BacMagic systems.

Figure 2. Streamlined baculovirus method
Product Information
0.5 µgBacMagic DNA
50 µlInsect GeneJuice Transfection Reagent
2 x 1 mlSf9 Cells
1 LBacVector® Insect Cell Medium
2 µgTransfection Control Plasmid
Quality LevelMQ100
Biological Information
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Multiple Storage Conditions
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Do not freeze Ok to freeze
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Supplemental Information
Global Trade Item Number
Catalogue Number GTIN
71546-3 04055977252415