Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

324713 Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus

View Products on Sigmaaldrich.com
324713
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
324713-50UG Glass bottle 50 μg
Description
OverviewNative endoproteinase Glu-C from Staphylococcus aureus. Serine protease that specifically hydrolyzes peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Catalogue Number324713
Brand Family Calbiochem®
SynonymsV8 Protease
References
ReferencesHoumard, J., and Drapeau, G.R. 1972. Proc. Natl. Acad. Sci. USA 69, 3506.
Product Information
CAS number66676-43-5
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-Phe-Leu-Glu-<i>p</i>NA per min at 25°C, pH 7.8.
EC number3.4.21.19
FormLyophilized
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥15 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 20-36/37/38-42/43

Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 22-26-36

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
324713-50UG 07790788049171

Documentation

Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus SDS

Title

Safety Data Sheet (SDS) 

Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus Certificates of Analysis

TitleLot Number
324713

References

Reference overview
Houmard, J., and Drapeau, G.R. 1972. Proc. Natl. Acad. Sci. USA 69, 3506.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-June-2008 RFH
SynonymsV8 Protease
DescriptionNative endoproteinase Glu-C from Staphylococcus aureus. Serine protease that specifically hydrolyzes peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin.
FormLyophilized
Recommended reaction conditions
Protocol 1. Dissolve the protein to be digested in buffer (e.g. 25 mM ammonium carbonate, pH 7.8). If the protein requires denaturation, add SDS (≤0.1%), guanidine hydrochloride (≤1 M), or urea (≤1 M) to the digestion buffer. (Note: if urea is used, also include 20 mM methylamine to avoid cyanate ion blockage of the N-terminus substrate protein. 2. Suggested working concentration is 1:20-1:100 (protease:protein by weight for sequence analysis). 3. Incubate for 2-18 h at 25°C.
CAS number66676-43-5
EC number3.4.21.19
Specific activity≥15 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-Phe-Leu-Glu-pNA per min at 25°C, pH 7.8.
SolubilityH₂O
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
Toxicity Harmful
ReferencesHoumard, J., and Drapeau, G.R. 1972. Proc. Natl. Acad. Sci. USA 69, 3506.

Related Products & Applications

Related Products

Catalogue Number Description
164598 Aminopeptidase M, Porcine Kidney
324682 Elastase, Porcine Pancreas, High Purity, Crystallized
324708 Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi
324715 Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes
516360 Pepsin, Porcine Stomach Mucosa
650211 Trypsin, Excision Grade, Bovine Pancreas

Categories

Life Science Research > Proteins and Enzymes > Other Enzymes