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361526 GSK-3β, Rabbit Skeletal Muscle, Recombinant, E. coli

361526
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
361526-5KU Plastic ampoule 5 ku
Description
OverviewRecombinant, rabbit glycogen synthase kinase 3β expressed in E. coli. Dual specificity kinase. One of several protein kinases that phosphorylates glycogen synthase. Other substrates include p90rsk, Tau, c-Jun, and CREB. Plays a key role in Wnt and insulin signaling.
Note: 1 KU = 1000 units.
Catalogue Number361526
Brand Family Calbiochem®
SynonymsGSK 3β-Isozyme, Rabbit Skeletal Muscle, Recombinant, E. coli
References
ReferencesDajani, R., et al. 2001. Cell 105, 721.
Wang, Q.M., et al. 1995. Biochem. Biophys. Res. Commun. 208, 796.
Fiol, C.J., et al. 1994. J. Biol. Chem. 269, 32187.
Wang, Q.M., et al. 1994. J. Biol. Chem. 269, 14566.
Wang, Q.M., et al. 1994. Biochemistry 33, 143.
deGroot, R.P., et al. 1993. Oncogene 8, 841.
Ishiguro, K., et al. 1993. FEBS Lett. 325, 167.
Product Information
Activity≥250,000 units/ml
Unit of DefinitionOne unit is defined as the amount of GSK-3 required to catalyze the transfer of 1 pmol phosphate to CREB phosphopeptide KRREILSRRPS(P)YRP (200 µM), in 1 min at 30°C in a reaction volume of 25 µl.
FormLiquid
FormulationIn 50 mM NaCl, 30 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, 0.03% BRIJ®-35 Detergent, pH 7.5
Quality LevelMQ100
Applications
ApplicationGSK-3β, Rabbit Skeletal MuscleHis-Tag®, Human, Recombinant, E. coli, is a dual specificity kinase that plays important roles in insulin- and Wnt-mediated signaling. It is inactivated by phosphorylation at Ser9.
Biological Information
Physicochemical Information
ContaminantsDNase: <10% conversion of RFI DNA to RFII and linear forms. Phosphatases and Proteases: none detected. RNases: <10% RNA degradation.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

GSK-3β, Rabbit Skeletal Muscle, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

GSK-3β, Rabbit Skeletal Muscle, Recombinant, E. coli Certificates of Analysis

TitleLot Number
361526

References

Reference overview
Dajani, R., et al. 2001. Cell 105, 721.
Wang, Q.M., et al. 1995. Biochem. Biophys. Res. Commun. 208, 796.
Fiol, C.J., et al. 1994. J. Biol. Chem. 269, 32187.
Wang, Q.M., et al. 1994. J. Biol. Chem. 269, 14566.
Wang, Q.M., et al. 1994. Biochemistry 33, 143.
deGroot, R.P., et al. 1993. Oncogene 8, 841.
Ishiguro, K., et al. 1993. FEBS Lett. 325, 167.

Brochure

Title
Alzheimer's Disease Brochure & Technical Guide
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-September-2007 RFH
SynonymsGSK 3β-Isozyme, Rabbit Skeletal Muscle, Recombinant, E. coli
DescriptionRecombinant, rabbit glycogen synthase kinase 3β expressed in E. coli. Glycogen synthase kinase 3β is a monomeric protein whose activity is independent of second messengers such as cAMP or IP3. Recent studies demonstrate that, like the dual specificity MAP kinase, it autophosphorylates on Ser, Thr, and Tyr. It phosphorylates several exogenous substrates, including protein phosphatase 1, the regulatory domain of protein kinase A, myelin basic protein, protein phosphatase inhibitor 2, c-jun, c-myb, c-myc, CREB, CREM, p90rsk, and microtubule-associated tau protein only on Ser and/or Thr, but not on Tyr residues. Plays a key role in Wnt and insulin signaling.
FormLiquid
FormulationIn 50 mM NaCl, 30 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, 0.03% BRIJ®-35 Detergent, pH 7.5
Recommended reaction conditions20 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 5 mM DTT, 200 µM ATP, 30°C. Optimal incubation times and enzyme concentrations must be determined empirically for each substrate.
ContaminantsDNase: <10% conversion of RFI DNA to RFII and linear forms. Phosphatases and Proteases: none detected. RNases: <10% RNA degradation.
Activity≥250,000 units/ml
Unit definitionOne unit is defined as the amount of GSK-3 required to catalyze the transfer of 1 pmol phosphate to CREB phosphopeptide KRREILSRRPS(P)YRP (200 µM), in 1 min at 30°C in a reaction volume of 25 µl.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesDajani, R., et al. 2001. Cell 105, 721.
Wang, Q.M., et al. 1995. Biochem. Biophys. Res. Commun. 208, 796.
Fiol, C.J., et al. 1994. J. Biol. Chem. 269, 32187.
Wang, Q.M., et al. 1994. J. Biol. Chem. 269, 14566.
Wang, Q.M., et al. 1994. Biochemistry 33, 143.
deGroot, R.P., et al. 1993. Oncogene 8, 841.
Ishiguro, K., et al. 1993. FEBS Lett. 325, 167.