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Immobilon® Membranes, Sandwiches and Blotting Filter Paper

Immobilon® PVDF membranes are the ideal transfer membranes for protein blotting applications.

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Overview

Specifications

Ordering Information

Immobilon® Membranes, Sandwiches and Blotting Filter PaperClear Sorting & Filtering Show Filter
Catalogue NumberDescriptionFilter DimensionsPack Size
IPVH07850Immobilon®-P PVDF Membrane 7 cm x 8.4 cm 50
IPVH08100Immobilon®-P PVDF Membrane 8 cm x 10 cm 10
IPVH08130Immobilon®-P PVDF Membrane 8.5 cm x 13.5 cm 10
IPVH09120Immobilon®-P PVDF Membrane 9 cm x 12 cm 10
IPVH10100Immobilon®-P PVDF Membrane 10 cm x 10 cm 10
IPVH15150Immobilon®-P PVDF Membrane 15 cm x 15 cm 10
IPVH20200Immobilon®-P PVDF Membrane 20 cm x 20 cm 10
IPVH304F0Immobilon®-P PVDF Membrane 26 cm x 26 cm 10
IPVH00010Immobilon®-P PVDF Membrane 27 cm x 3.75 m 1
ISEQ07850Immobilon®-PSQ PVDF Membrane 7 cm x 8.4 cm 50
ISEQ08100Immobilon®-PSQ PVDF Membrane 8 cm x 10 cm 10
ISEQ08130Immobilon®-PSQ PVDF Membrane 8.5 cm x 13.5 cm 10
ISEQ09120Immobilon®-PSQ PVDF Membrane 9 cm x 12 cm 10
ISEQ10100Immobilon®-PSQ PVDF Membrane 10 cm x 10 cm 10
ISEQ15150Immobilon®-PSQ PVDF Membrane 15 cm x 15 cm 10
ISEQ20200Immobilon®-PSQ PVDF Membrane 20 cm x 20 cm 10
ISEQ26260Immobilon®-PSQ PVDF Membrane 26 cm x 26 cm 10
ISEQ00010Immobilon®-PSQ PVDF Membrane 27 cm x 3.75 m 1
IPFL10100Immobilon®-FL PVDF Membrane 10 cm x 10 cm 10
IPFL20200Immobilon®-FL PVDF Membrane 20 cm x 20 cm 10
IPFL00010Immobilon®-FL PVDF Membrane 27 cm x 3.75 m 1
IPSN07852Immobilon®-P Blotting Sandwich 7 cm x 8.4 cm 20
IPSN08132Immobilon®-P Blotting Sandwich 8.5 cm x 13.5 cm 20
IBFP0785CImmobilon® Blotting Filter Paper 7 cm x 8.4 cm 100
IBFP0813CImmobilon® Blotting Filter Paper 8.5 cm x 13.5 cm 100
IPFL00005Immobilon®-FL PVDF Membrane 27 cm x 1.875 m 1
IPVH00005Immobilon®-P PVDF Membrane 27 cm x 1.875 m 1
ISEQ00005Immobilon®-PSQ PVDF Membrane 27 cm x 1.875 m 1
IEVH00005Immobilon®-E PVDF Membrane 27 cm x 1.875 m 1
IEVH07804Immobilon®-E PVDF Membrane 7 cm x 8.4 cm 4
IEVH07850Immobilon®-E PVDF Membrane 7 cm x 8.4 cm 50
IEVH08100Immobilon®-E PVDF Membrane 8 cm x 10 cm 10
IEVH09120Immobilon®-E PVDF Membrane 9 cm x 12 cm 10
IEVH10100Immobilon®-E PVDF Membrane 10 cm x 10 cm 10
IESN07852Immobilon®-E Blotting Sandwich 7.0 cm x 8.4 cm 20
IESN08132Immobilon®-E Blotting Sandwich 8.5 cm x 13.5 cm 20
IMDISPImmobilon® NOW Dispenser 1
IPVH85RImmobilon®-P Membrane, PVDF, 0.45 µm, 8.5 cm x 10 m roll 8.5 cm x 10 m 1
ISEQ85RImmobilon®-PSQ Membrane, PVDF, 0.2 µm, 8.5 cm x 10 m roll 8.5 cm x 10 m 1
IEVH85RImmobilon®-E PVDF Membrane 8.5 cm x 10 m 1
IPFL07810Immobilon®-FL PVDF Membrane 7 cm x 8.4 cm 10

*Roll

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Documentation

References

Reference overviewApplication
Mobile Phase Preparation for UHPLC: Membrane Filtration Method Affects System Performance and Leaching of Extractable Impurities
Subodh Kulkarn(1), Jesmi George(2) and Vivek Joshi(2) (1) Millipore India Pvt. Ltd., Bioscience Division, 50A, 2nd Phase, Ring Road, Peenya, Bangalore, India 560058 (2) Millipore Corp., Bioscience Division, 17 Cherry Hill Drive, Danvers, MA 01923
LCGC  2010

Show Abstract Full Text Article
Quantitation of Protein on gels and blots by infrared fluorescence of Coomassie blue and fast green
Luo S., Wehr N.B., Levine R.L.
Analytical Biochemistry:350 (2006):233-238  2006

Immunoblotting (Western)
Role of the Small Heat Shock Proteins in Regulating Vascular Smooth Muscle Tone
McLemore E.C., Tessier D.J., Thresher J., Komalavilas P., Brophy C.M
J. Am. Coll. Surg. 2005, Vol 201 (1):30-36  2005

Pre-B-cell colony-enhancing factor is a secreted cytokine-like protein from the human amniotic epithelium.
Ognjanovic S, Ku TL, Bryant-Greenwood GD.
Am J Obstet Gynecol. 2005 Jul;193(1):273-82  2005

Western Blotting
A high-affinity reversible protein stain for Western blots
Antharavally B.S., Carter, B., Bell, P.A., Mallia K.
Analytical Biochemistry 2004,Vol 329:276-280  2004

Biochemical analysis of GABA receptor subunits alpha 1, alpha 5, beta1 beta2 in the hippocampus of patients with Alzheimer's disease neurophathology
Rissman, R.A., Mishizen-Eberz A.J. N.,Wolfe, C.B.B., DeBlas A.L., Miralles C.P., Ikonomovic M.D., armstrong D.M.
Neuroscience 120 (2003) 295-705  2003

Western Blotting
Proteomics reveals protein profile changes in doxorubicin treated MCF7 human breast cancer cells
Cheng S.T., Pan T, L., Tsai Y.Ch, Huang C. M.
Cancer letters 2002. vol 181:95-107  2002

Towards proteome-wide production of monoclonal antibody by phage display.
Bin Liu, Lan Huang, Carina Sihlbom, Al Burlingame and James D. Marks.
J Mol Biol. 2002 Feb 1;315(5):1063-73  2002

Mass Spectrometry Sample Prep
Characterization of retinoic acid receptor-deficient keratinocytes.
Goyette Philippe; Chen Chang Feng; Wang Wei; Seguin Francois; Lohnes David(a)
Journal of Biological Chemistry v 275 pg 16497-16505 June 2, 2000  2000

Protection of renal inner medullary epithelial cells from apoptosis by hypertonic stress-induced p53 activation
Dmitrieva Natalia(a); Kultz Dietmar; Michea Luis; Ferraris Joan; Burg Maurice ; Journal of Biological Chemistry v 275, pg 18243-18247 June 16, 2000
Journal of Biological Chemistry v 275, pg 18243-18247 June 16, 2000  2000

FAQ

QuestionAnswer
What is the difference between Immobilon–FL and Immobilon-P?Both membranes are made from PVDF. The difference in background fluorescence is due to proprietary modifications in the membrane manufacturing process.
How does the protein binding capacity and protein retention of Immobilon-FL compare to Immobilon-P?Immobilon-FL’s protein binding capacity and protein retention are comparable to Immobilon-P.
Is Immobilon-FL better than Nitrocellulose membrane for Fluorescence detection?Yes. Background fluorescence of Immobilon-FL is typically 2-5X lower than that of nitrocellulose, thus improving signal-to-noise ratio (sensitivity). Nitrocellulose membranes have other disadvantages. If allowed to dry out, they become brittle, tend to fracture and are difficult to handle. They are not recommended for stripping and re-probing. Nitrocellulose blots need to be scanned as soon as possible after detection, as diffusion of signal on a wet membrane may also occur.
What fluorescence-based detection methods can be used with Immobilon-FL?Immobilon-FL can be used in Western blotting applications using either fluorescent dye-conjugated antibodies or chemi-fluorescence substrates. Western blots can be imaged in the visible or IR range. Single color detection or multiplexing for co-localization studies can be performed efficiently on Immobilon-FL Fluorescent proteins, e.g. green fluorescent protein, (GFP) or proteins tagged with GFP, blotted onto the membrane can be readily detected as well.
What is the level of detection sensitivity when using Immobilon-FL?Immobilon-FL exhibits the lowest background fluorescence of any blotting membrane thus improving the signal-to-noise ratio (sensitivity) of any fluorescent dye. The fluorescence signal is dependent on the fluorescent dye used, thus the ultimate sensitivity of detection is determined by the dye itself. With Immobilon-FL the sensitivity is not limited by the membrane, e.g., detection of proteins in the low picogram level has been observed with QDot® Nanocrystals fluorescent-tagged antibodies on Immobilon-FL.
What fluorescent dyes are recommended to use with Immobilon-FL?Immobilon-FL membrane exhibits very low background fluorescence over a broad range of wavelengths and can be used with all visible and infrared fluorescent dyes. Generally, dyes with larger Stokes shift (greater separation between absorption and emission wavelengths) are preferred in western blotting applications using fluorescence-based detection. NOTE: To prevent photo-bleaching, protect the membrane from light during secondary antibody incubations and washes until the membrane is ready to be scanned.
What are the recommendations for two-color western / multiplex detection?The two antibodies must be derived from different host species, so that they can be differentiated by secondary antibodies of different specificities. Before combining the two primary antibodies test their banding patterns on separate blots to know where to expect the bands. Use highly adsorbed cross-adsorbed secondary antibodies in two-color detection. Refer to the directions provided with the detection reagents for additional details.
How long does the fluorescent signal stay on the developed blot?The fluorescent signal from fluorescence-tagged antibodies will remain stable on the membrane for several months, or longer, when stored protected from light. The membranes may be stored dry or in PBS at 4ºC. The fluorescent signal from chemi-fluorescent substrates does not remain for long so blots using these substrates need to be scanned immediately.
Are there specific recommendations on using 'low fluorescent’ reagents (buffers, blocking agents, etc.) with Immobilon-FL?Immobilon-FL has been tested with the most commonly-used blocking reagents and buffers such as TBST, PBST, dry milk, BSA, casein and buffers recommended by the fluorescence scanner manufacturers, e.g., Licor Odyssey buffers. All were found to be compatible with fluorescent detection on Immobilon-FL. However, as in any western blotting experiment, optimization of blocking reagents and buffers may be required, depending on the nature of the antigen, antibodies and fluorescent dyes used. The use of freshly prepared buffers is always recommended.
Can I use Tween-20 in my buffers in western blotting with Immobilon-FL?Do not expose membrane to Tween-20 until after the blocking step is completed. Presence of Tween-20 during blocking may increase background fluorescence.