Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on More

Millicell® Cell Culture Inserts

Optimized cell growth, attachment and differentiation

Related Resources



Ordering Information

Millicell Standing InsertsClear Sorting & Filtering Show Filter
Catalogue NumberMediaChemistryPore SizeFiltration AreaDevice ConfigurationPack Size
PICM0RG50Biopore® Hydrophilic Polytetrafluoroethylene (PTFE) 0.4 µm 4.2 cm² 6-well plate 50
PIHA01250MF-Millipore Mixed Cellulose Esters (MCE) 0.45 µm 0.6 cm² 24-well plate 50
PIHA03050MF-Millipore Mixed Cellulose Esters (MCE) 0.45 µm 4.2 cm² 6-well plate 50
PICM01250Biopore® Hydrophilic Polytetrafluoroethylene (PTFE) 0.4 µm 0.6 cm² 24-well plate 50
PICM03050Biopore® Hydrophilic Polytetrafluoroethylene (PTFE) 0.4 µm 4.2 cm² 6-well plate 50
PIHP01250Isopore® Polycarbonate (PC) 0.4 µm 0.6 cm² 24-well plate 50
PIHP03050Isopore® Polycarbonate (PC) 0.4 µm 4.2 cm² 6-well plate 50
PITP01250Isopore® Polycarbonate (PC) 3.0 µm 0.6 cm² 24-well plate 50
PIXP01250Isopore® Polycarbonate (PC) 12.0 µm 0.6 cm² 24-well plate 50
PI8P01250Isopore® Polycarbonate (PC) 8.0 µm 0.6 cm² 24-well plate 50

Back To Top

Millicell Hanging InsertsClear Sorting & Filtering Show Filter
Catalogue NumberChemistryPore SizeFiltration AreaDevice ConfigurationPack Size
MCEP06H48Polyethylene Terephthalate (PET) 8.0 µm 4.5 cm² 6-well plate 48
MCEP12H48Polyethylene Terephthalate (PET) 8.0 µm 1.1 cm² 12-well plate 48
MCEP24H48Polyethylene Terephthalate (PET) 8.0 µm 0.3 cm² 24-well plate 48
MCHT06H48Polyethylene Terephthalate (PET) 0.4 µm 4.5 cm² 6-well plate 48
MCHT12H48Polyethylene Terephthalate (PET) 0.4 µm 1.1 cm² 12-well plate 48
MCHT24H48Polyethylene Terephthalate (PET) 0.4 µm 0.3 cm² 24-well plate 48
MCMP06H48Polyethylene Terephthalate (PET) 5.0 µm 4.5 cm² 6-well plate 48
MCMP12H48Polyethylene Terephthalate (PET) 5.0 µm 1.1 cm² 12-well plate 48
MCMP24H48Polyethylene Terephthalate (PET) 5.0 µm 0.3 cm² 24-well plate 48
MCRP06H48Polyethylene Terephthalate (PET) 1.0 µm 4.5 cm² 6-well plate 48
MCRP12H48Polyethylene Terephthalate (PET) 1.0 µm 1.1 cm² 12-well plate 48
MCRP24H48Polyethylene Terephthalate (PET) 1.0 µm 0.3 cm² 24-well plate 48
MCSP06H48Polyethylene Terephthalate (PET) 3.0 µm 4.5 cm² 6-well plate 48
MCSP12H48Polyethylene Terephthalate (PET) 3.0 µm 1.1 cm² 12-well plate 48
MCSP24H48Polyethylene Terephthalate (PET) 3.0 µm 0.3 cm² 24-well plate 48

* For attachment-dependent cells, this membrane needs to be coated with an extra cellular matrix.

Back To Top

AccessoriesClear Sorting & Filtering Show Filter
Catalogue NumberDescriptionPack Size
MERS00002Millicell ERS-2 Voltohmmeter 1
MERSSTX01Replacement Electrodes, 1 pair 1

Back To Top



Advanced cell culture systems for every challenge.
MultiScreen® and Millicell® Filter Plates for Assay Development

Technical Info

Millicell Organotypic Cell Culture Procedure


Reference overviewApplication
Propagation of human embryonic stem cells in a microporous membrane-based indirect co-culture system
Kelsey Albert, Steven Sheridan, Louise Laurent, Igor Ulitsky, Ron Shamir, Jeanne Loring, & Raj R. Rao
Biochemical and Biophysical Research Communications  2010

Astrocyte growth effects of vascular endothelial growth factor (VEGF) application to perinatal neocortical explants: Receptor mediation and signal transduction pathways
Nina Mani, Alfia Khaibullina, Janette Krum and Jeffrey Rosenstein
Experimental Neurology 192 (2005); 394-406  2005

Cell Culture
Subcellular localisation of recombinant a and g-synuclein
Christian Specht, Cezar Tigaret, George Rast, Agnea Thalhammer, York Rudhard and Ralf Schoepfer
Mol. Cell. Neurosci., 28 (2005); 326-334  2005

Cell Culture
Establishment of the organotypic model of amyotrophic lateral sclerosis from the SD rats' spinal cord
Diao ZY, et. al,Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese.
Beijing Da Xue Xue Bao. 2005 Apr 18;37(2):134-8. Chinese.  2005

Cell Culture
Development of an in vitro blood-brain barrier model-cytotoxicity of mercury and aluminum.
Toimela, T et. al.,Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.
Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.  2004

Morphological differentiation of bone marrow stromal cells into neuron-like cells after co-culture with hippocampal slice
Abouelfetouh, Ayman, et al
Brain Research (2004), Volume 1029, Issue 1 pp 114-119  2004

Cell Culture
Effect of sodium bicarbonate on extracellular pH, matrix accumulation, and morphology of cultured articular chondrocytes.
Waldman SD, Couto DC, Omelon SJ, Kandel RA
Tissue Engineering (2004), Nov-Dec;10(11-12):1633-40  2004

Neural stem cells protect against glutamate-induced excitotoxicitiy and promote survival of injured motor neurons through the secretion of neurotrophic factors
Jeronia Llado, Christine Haenggeli, Nicholas Maragakis, Evan Snyder and Jeffrey Rothstein
Mol. Cell. Neurosci. , 27 (2004); 322-331  2004

Cell Culture
FGF-10 plays an essential role in the growth of the fetal prostate.
Annemarie A. Donjacour, Axel A. Thomson and Gerald R. Cunha
Developmental Biology 261 (1): 39-54  2003

Cell Culture
Changes in lymphokine receptor expression and fatty acid composition of phospholipids and triacylglycerols in rat adipocytes associated with lymph nodes following a transient immune challenge.
J. D. Priddle, C. A. Mattacks, D. A. Sadler, H. A. MacQueen and C. M. Pond
Cell Biology International 27 (1): 23-29  2003

Cell Differentiation


Why do the electrodes need to be replaced every six months?In general, electrodes have a 6 month lifetime if they are used continuously. The electrodes have a silver-silver chloride pellete that is depleted everytime it is used. It is the silver silver chloride pellete that creates the potential.
What voltage value can I expect for my cell type?We DO NOT recommend using this function on the instrument. The Millicell-ERS will make voltage readings on only a few kinds of high-voltage generating cells (for example, it will not work on MDCK cells). We do have information on epithelial cells which typically have voltage values of 0-30mV.
What is the porosity of the Millicell Culture Plate Inserts?The porosity of the Millicell Insert membranes are as follows: HATF - approx 80% CM - approx 80% PC and PCF - approx 10%
What is the thickness of the Millicell-CM membrane?The thickness of the Millicell-CM memnbrane is approximately 50 um.
What is the difference between Millicell PC and PCF?Both Millicells have polycarbonate membranes and are frequently used in chemotaxis and transport studies. PC's are recommended ONLY for suspension cell culture. PCF's are tissue culture treated and therefore recommended for attachment dependent cell culture.
What is the advantage of using Millicell for cell culture applications ?Studies have shown that cells actually grow better on microporous membranes than on the artificially smooth surfaces of standard cell culture plasticware. We have found that cells grown on Millicell Cell Culture Plate Inserts produce higher cell densities and greater differentiation than cells grown in standard setups. Cells can be constantly bathed and fed on both their apical and basolateral surfaces and ions and molecules can pass freely across the membrane. Millicells also offer the researcher a more humane approach to animal testing since cell culturing with the units can offer alternatives to live animal studies for many toxicological studies.
Are the Millicell PCF inserts tissue culture treated on both sides of the membrane?Yes, they are tissue culture treated on both sides of the membrane. This allows for attachment- dependent cell culture on both sides of the membrane.
Are the Millicell units sterile and, if so, how are they sterilized?The Millicell units are sold sterile in individual blister packs. The HA and CM units are ETO sterilized and the PC and PCF are Gamma Irradiated.
Can cells grown on Isopore (polycarbonate) membrane be viewed under a microscope?Yes, we can observe the cells seeding on the membrane of pore size 1 um.
Can I make membrane potential measurements with the Millicell-ERS?Yes, membrane potential measurements can be made with the ERS. Short circuit potential, however, cannot be done.

User Guides

Millicell 24 and Millicell 96
Millicell Culture Plate Inserts
Millicell Hanging Cell Culture Inserts
Millicell-CM Low Height Culture Plate Inserts User Guide
Millicell-ERS User Guide
Millicell® Hanging Cell Culture Inserts