A Stable RP Stationary Phase, Optimized for Applications up to pH12 |
Request Information |
Merck’s Aluspher® RP-select B HPLC column is an alkali-stable reversed-phase stationary phase designed for the chromatographic separation of neutral, basic and acidic compounds under neutral conditions. Due to its stability in the pH range of 2-12, Aluspher® RP-select B allows the use of basic eluents, such as NaOH. This suppresses the ionization of basic compounds, and thus avoids peak tailing. Furthermore, advanced formulation technology has enabled the production of spherical alumina particles as a base for Aluspher® 100 RP-select B. The combination of alumina with alkaline eluents has led to new applications in HPLC.
Specifications of Aluspher®
Column |
Aluspher® 100 RP-select B (5 μm) LiChroCART® 250-4 |
|---|---|
Mobile phase |
Acetonitrile/Water 35/65 (v/v) |
Flow rate |
1.0 ml/min |
Detection |
UV 220 nm |
| Temperature | Room temperature |
| Sample | 1. Levamisole 2. 5-Methyl-5-phenyldantoin (MPH) 3. 5-Phenyl-5-(2-pyridyl)-hydantoin (PPH) 4. 5-(p-Methylphenyl)-5-phenylhydantoin (MPH) 5. Diazepam |
 |
Column |
Aluspher® 100 RP-select B (5 μm) LiChroCART® 250-4 |
|---|---|
Mobile phase |
Methanol/0.025 M NaOH 25/75 (v/v) |
Flow rate |
1.0 ml/min |
Detection |
UV 215 nm |
| Temperature | Room temperature |
| Sample | 1. Amphetamine 2. p-Methoxyamphetamine (PMA) 3. Methylendioxyamphetamine (MDA) 4. Methamphetamine 5. Methylendioxymethamphetamine (MDMA) 6. Ethylamphetamine 7. Methylendioxyethylamphetamine (MDEA) |
 |
Column |
Aluspher® 100 RP-select B (5 μm) LiChroCART® 250-4 |
|---|---|
Mobile phase |
Methanol/0.025 M NaOH 50/50 (v/v) |
Flow rate |
1.0 ml/min |
Detection |
UV 215 nm |
| Temperature | Room temperature |
| Sample | 1. Sotalol 2. Atenolol 3. Metoprolol 4. Bisoprolol |
 |
Merck offers an extensive range of services, information and tools to support you in your analysis. Use the links below to learn more.
