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MF-Millipore™ Membrane Filters

Mixed cellulose esters

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Standard MF-Millipore Membranes, white, plain · Catalogue No.Borrar Clasificación y Filtrado Show Filter
Número de referenciaTamaño de poroDiámetro del filtro (⌀)Tamaño de envase
VSWP013000.025 µm 13 mm 100 Precios y disponibilidad
VSWP025000.025 µm 25 mm 100 Precios y disponibilidad
VSWP047000.025 µm 47 mm 100 Precios y disponibilidad
VMWP025000.05 µm 25 mm 100 Precios y disponibilidad
VMWP047000.05 µm 47 mm 100 Precios y disponibilidad
VCWP025000.1 µm 25 mm 100 Precios y disponibilidad
VCWP047000.1 µm 47 mm 100 Precios y disponibilidad
GSWP013000.22 µm 13 mm 100 Precios y disponibilidad
GSWP025000.22 µm 25 mm 100 Precios y disponibilidad
GSWP047000.22 µm 47 mm 100 Precios y disponibilidad
GSWP090000.22 µm 90 mm 100 Precios y disponibilidad
GSWP142500.22 µm 142 mm 50 Precios y disponibilidad
GSWP293250.22 µm 293 mm 25 Precios y disponibilidad
HAWP013000.45 µm 13 mm 100 Precios y disponibilidad
HAWP037000.45 µm 37 mm 100 Precios y disponibilidad
HAWP047000.45 µm 47 mm 100 Precios y disponibilidad
HAWP04700F10.45 µm 47 mm Hydrophilic, 0.45µm, White Plain 47 mm, 100 Precios y disponibilidad
HAWP025000.45 µm 25 mm 100 Precios y disponibilidad
HAWP090000.45 µm 90 mm 100 Precios y disponibilidad
HAWP142500.45 µm 142 mm 50 Precios y disponibilidad
HAWP293250.45 µm 293 mm 25 Precios y disponibilidad
DAWP025000.65 µm 25 mm 100 Precios y disponibilidad
AAWP025000.8 µm 25 mm 100 Precios y disponibilidad
AAWP037000.8 µm 37 mm 100 Precios y disponibilidad
AAWP037PM0.8 µm 37 mm 50 pr Precios y disponibilidad
AAWP037P00.8 µm 37 mm 100 Precios y disponibilidad
AAWP047000.8 µm 47 mm 100 Precios y disponibilidad
AAWP0470M0.8 µm 47 mm 50 pr Precios y disponibilidad
AAWP090000.8 µm 90 mm 100 Precios y disponibilidad
AAWP142500.8 µm 142 mm 50 Precios y disponibilidad
AAWP293250.8 µm 293 mm 25 Precios y disponibilidad
RAWP013001.2 µm 13 mm 100 Precios y disponibilidad
RAWP025001.2 µm 25 mm 100 Precios y disponibilidad
RAWP047001.2 µm 47 mm 100 Precios y disponibilidad
RAWP090251.2 µm 90 mm 25 Precios y disponibilidad
RAWP293251.2 µm 293 mm 25 Precios y disponibilidad
SSWP047003.0 µm 47 mm 100 Precios y disponibilidad
SSWP025003.0 µm 25 mm 100 Precios y disponibilidad
SSWP090253.0 µm 90 mm 25 Precios y disponibilidad
SSWP142503.0 µm 142 mm 50 Precios y disponibilidad
SSWP293253.0 µm 293 mm 25 Precios y disponibilidad
SMWP025005.0 µm 25 mm 100 Precios y disponibilidad
SMWP037005.0 µm 37 mm 100 Precios y disponibilidad
SMWP047005.0 µm 47 mm 100 Precios y disponibilidad
SMWP293255.0 µm 293 mm 25 Precios y disponibilidad
SCWP013008.0 µm 13 mm 100 Precios y disponibilidad
SCWP025008.0 µm 25 mm 100 Precios y disponibilidad
SCWP047008.0 µm 47 mm 100 Precios y disponibilidad

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Standard MF-Millipore Membranes, white, griddedBorrar Clasificación y Filtrado Show Filter
Número de referenciaTamaño de poroDiámetro del filtro (⌀)Tamaño de envase
HAWG025000.45 µm 25 mm 100 Precios y disponibilidad
HAWG047000.45 µm 47 mm 100 Precios y disponibilidad
AAWG047000.8 µm 47 mm 100 Precios y disponibilidad
RAWG047001.2 µm 47 mm 100 Precios y disponibilidad

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Standard MF-Millipore Membranes, black, plainBorrar Clasificación y Filtrado Show Filter
Número de referenciaTamaño de poroDiámetro del filtro (⌀)Tamaño de envase
HABP025000.45 µm 25 mm 100 Precios y disponibilidad
HABP047000.45 µm 47 mm 100 Precios y disponibilidad
AABP047000.8 µm 47 mm 100 Precios y disponibilidad

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Standard MF-Millipore Membranes, black, griddedBorrar Clasificación y Filtrado Show Filter
Número de referenciaTamaño de poroDiámetro del filtro (⌀)Tamaño de envase
AABG025000.8 µm 25 mm 100 Precios y disponibilidad
AABG047000.8 µm 47 mm 100 Precios y disponibilidad
HABG047000.45 µm 47 mm 100 Precios y disponibilidad

(1)Monitor refills with thin absorbent pads for aerosol monitoring.
(2)Matched weight filter pairs.
(3)Monitor refills with thick absorbent pads for liquid monitoring.
(4)Monitor refills (matched weight pairs) with thick absorbent pads for liquid monitoring.
(5)Cut from specifically selected and controlled roll stock to avoid contamination by fibers. For asbestos monitoring applications.

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MF-Millipore Membranes without Triton surfactant, white, plainBorrar Clasificación y Filtrado Show Filter
Número de referenciaTamaño de poroDiámetro del filtro (⌀)Tamaño de envase
GSTF047000.22 µm 47 mm 100 Precios y disponibilidad
HATF013000.45 µm 13 mm 100 Precios y disponibilidad
HATF047000.45 µm 47 mm 100 Precios y disponibilidad
HATF082500.45 µm 82 mm 50 Precios y disponibilidad

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AccessoryBorrar Clasificación y Filtrado Show Filter
Número de referenciaDescripciónTamaño de envase
XX6200006PPinzas de bordes planos, acero inoxidable 3 Precios y disponibilidad

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Documentation

Referencias bibliográficas

Visión general referenciasAplicación
Mobile Phase Preparation for UHPLC: Membrane Filtration Method Affects System Performance and Leaching of Extractable Impurities
Subodh Kulkarn(1), Jesmi George(2) and Vivek Joshi(2) (1) Millipore India Pvt. Ltd., Bioscience Division, 50A, 2nd Phase, Ring Road, Peenya, Bangalore, India 560058 (2) Millipore Corp., Bioscience Division, 17 Cherry Hill Drive, Danvers, MA 01923
LCGC  2009

Mostrar resumen Artículo Texto completo
Direct Effect of Vanadium on Citrate Uptake by Rat Renal Brush Border Membrane Vesicles (BBMV)
Kazuhiro Sato, et al.
Industrial Health , 40, 278-281, 2002, internal ref BB02  2002

Effect of Platinum Coordination Complex (PtCx) on Citrate Uptake by Rat Renal Bush Border Membrane vesicles (BBMV):Direct Effect of Carboplatin
Kazuhiro Sato, et al.
Industrial Health, 39, 21-31, 2001; internal ref BB01  2001

A sensitive Elispot assay to detect low-frequency human T-lymphocytes
McCutcheon M, Wehner N, Wensky A et al.
J Immunol Methods 210(2): 149-166.  1997

ELISPOT Assays
Direct effect of cadmium on citrate uptake by isolated rat renal brush border membrane cesicles
Kazuhiro Sato, et al.
Toxicology Letters, 80, 161-165, 1995, internal ref BB06  1994

Stoichiometry of Na+-Succinate Cotransport in Renla Brush-Border Membranes
Stephen H. at al.,Journal of Biological Chemistry, 257, 4, 1773-1778, 1982, Internal ref bb03
Journal of Biological Chemistry, 257, 4, 1773-1778, 1982, Internal ref bb03  1981

Preguntas frecuentes

PreguntaRespuesta
What would you recommend to dissolve MF ( mixed cellulose ester ) filters?Many solvents are possible. A popular choice is acetone, which is non-toxic and highly volatile so it is easy to evaporate (but quite a flammable). Another choice is NMP (n-methylpyrrolidinone), which is not very volatile at room temperature. The selection is often based on what is to be done after the dissolution.
How do I clear my Millipore filters?It is possible to clear Millipore filters by choosing a clearing agent with the same refractive index as the membrane. This is called the PORE FILLING TECHNIQUE. Filters appear opaque because of the diffraction of light through the tortuous pores. Filling the pores with a liquid that has the same refractive index as the membrane (ex. xylene for MF filters) allows light to pass through the filter at a uniform speed thus rendering the filter transparent. This technique can be used for most membrane filters with a single refractive index. The refractive index for Durapore membrane is 1.42 and for MF membrane its 1.50.

Please note that this does not work for polycarbonate membranes because these membranes have more than one refractive index (1.62 and 1.58). If desired, the porous structure can be dissolved from Isopore polycarbonates by dissolving the filter in chloroform, methylene chloride or 1-methyl-2 pyrrolidone.
What is the blue separation paper between my filters?It is a parchmentalized paper specially formulated for Millipore Corporation.
How do I tell the membrane from the separator?The blue paper is the separator.
How do I assemble a diffusion chamber containing tissue for implantation?
  1. Wet a single membrane filter (either GSWP 013 00 or HAWP 013 00) with Milli-Q water and blot it to absorbent paper to remove excess water.
  2. Glue the filter to one side of a diffusion chamber ring without hole (catalog number PR00 014 00), using MF cement (catalog number XX70 000 00), and let dry.
  3. Sterilize this assembly, along with another membrane filter of the same type, by exposure to ethylene oxide gas or ultraviolet light.
  4. Moisten the half-completed chamber and the separate sterile filter with a sterile physiological solution, and aseptically place the tissue to be studied in the half-completed chamber assembly.
  5. Glue the cover filter in place using sterile technique.
What are the dimensions and width of the grids on the Millipore 47mm gridded membranes?The grids are 3.08mm x 3.08mm square. A grid line is 44um in width and there is are average of 169 gridded squares per filter. Please note however that the actual filtration area and therefore the total number of squares in that area is dependent on the filter holder. For filter holders with 9.6sq.cm of filtration area there are 100 squares available. For filter holders with 13.8 sq.cm of filtration area the number of available grids are approximately 140.
What filter do you recommend for dialyzing 5-100ul sample volumes of proteins and nucleic acids (i.e. to perform a drop dialysis technique)?With the Millipore drop dialysis technique 0.025 um or 0.05 um MF mixed cellulose ester membranes are commonly used. This simple- to-use technique takes advantage of the varying diffusion rates of different size molecules to quickly dialyze small volume samples. The technique involves filling the bottom of a Petri dish with dialysate and floating the membrane filter disc, shiny side up, on the surface of the solution. You then deposit 5-100 ul samples on the center of the disc with a micropipet. use of dye markers in the sample may help to determine the dialysis time required. Most samples are dialyzed in less than 30 minutes. then simply recover the desalted sample with a micropipet.
How do I assemble a diffusion chamber containing suspension cells for implantation?Wet two membrane filters (either GSWP 013 00 or HAWP 013 00) with Milli-Q water and blot them to absorbent paper to remove excess water. Glue a moist filter to each side of the diffusion chamber ring with hole (catalog number PR00 014 01) using MF cement (catalog number XX70 000 00). Sterilize the dried diffusion chamber with ethylene oxide gas or ultraviolet light. After sterilization, inject the cells through the hole in the plastic ring, and plug the hole with the nylon thread which comes with the rings (catalog number PR00 000 00 if ordered separately).
Which membrane should I use to filter my solution?For general filtration use MCE. When you are looking for the lowest protein binding membrane use Durapore. For speed, and when filtering serum solutions use Millipore Express.
Which side of the membrane should I use, the shiny or dull side?Most researchers may not even notice that there is a "sidedness" to filters, and, for most applications, orientation will not affect filter performance. However, membranes do have a slightly asymmetric pore structure: the shiny side of the membrane is the "tighter" side. In some applications, you can take advantage of this difference by selecting a specific filter orientation. ultrafiltration membranes should always be used shiny side up, regardless of application for drop dialysis ( a buffer exchange technique in which a few drops of DNA or protein are placed in a 0.05 or 0.025 um filter and floated on a buffer solution), apply the sample to the shiny side of the filter and float the filter dull side to the buffer. This measure will enhance buffer exchange and discourage sample loss. The Millipore Express and Express Plus membranees are also sided - these membranes should be used shiny side facing down.
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