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Merck

302643

Trypan Blue

≥80% (HPLC), Dye content 60 %

Synonym(s):

Direct blue 14

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About This Item

Linear Formula:
C34H24N6O14S4Na4
CAS Number:
72-57-1
Molecular Weight:
960.81
Colour Index Number:
23850
NACRES:
NA.47
PubChem Substance ID:
24858268
UNSPSC Code:
12171500
EC Number:
200-786-7
MDL number:
MFCD00003969
Beilstein/REAXYS Number:
4360496

Product Name

Trypan Blue, ≥80% (HPLC), Dye content 60 %

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

assay

≥80% (HPLC)

form

powder

composition

Dye content, 60%

technique(s)

microbe id | staining: suitable

color

dark green to brown, or Brown-Black

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

λmax

607 nm

ε (extinction coefficient)

≥60000 at 603-607 nm in methanol at 0.01 g/L
60000 at 603-607 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

Gene Information

human ... PTPN1(5770), PTPRF(5792)

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Application

Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Biochem/physiol Actions

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.
Trypan blue is routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains. It is suitable for use in cell viability assay:
  • to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
  • to stain plant tissue in order to visualize cell death.

General description

Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT0336
SHBS8023
SHBS3903
SHBS0248
SHBR4961

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Ocular toxicity study of trypan blue injected into the vitreous cavity of rabbit eyes
Graefe'S Archive For Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 239, 698-704 (2001)
Thangaraj Parimelazhagan
Pharmacological Assays of Plant-Based Natural Products (2013)
Role of caspase-10 in the death of acute leukemia cells
Guo W
Oncology Letters, 12, 1623-1629 (2016)
Lack of Bax Prevents Influenza A Virus-Induced Apoptosis and Causes Diminished Viral Replication
McLean J E, et.al.
Journal of Virology, 83, 8233-8246 (2009)
Jeffrey E McLean et al.
Journal of virology, 83(16), 8233-8246 (2009-06-06)
The ectopic overexpression of Bcl-2 restricts both influenza A virus-induced apoptosis and influenza A virus replication in MDCK cells, thus suggesting a role for Bcl-2 family members during infection. Here we report that influenza A virus cannot establish an apoptotic
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