A3699

Acrylamide/Bis-acrylamide solution

Name: Acrylamide/Bis-acrylamide solution
Brand: SIGMA

30%, BioReagent, suitable for electrophoresis, 37.5:1

Synonym(s):

30% Acrylamide/Bisacrylamide 37.5:1, Polyacrylamide solution

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All Photos(1)

About This Item

UNSPSC Code:

41105319

PubChem Substance ID:

329770843

NACRES:

NA.25

MDL number:

MFCD00080848

Product Name

Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 37.5:1

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

sterility

0.2 μm filtered

product line

BioReagent

form

liquid

feed ratio

37.5:1

technique(s)

electrophoresis: suitable

suitability

suitable for electrophoresis

storage temp.

2-8°C
2-8°C

Quality Level

100

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Application

Acrylamide/Bis-acrylamide, 30% solution has been used:

in the preparation of polyacrylamide gel for photobleaching experiment
as a constituent in the preparation of gel precursor solution and pore-gradient microgel precursor solution
in the preparation of polyacrylamide gels during western blot analyses

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H302 + H332,H315,H317,H319,H340,H350,H361f,H372

pcodes

P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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