A6132

Apyrase from potatoes

Name: Apyrase from potatoes
Brand: SIGMA

ATPase ≥3.0 units/mg protein, lyophilized powder

Synonym(s):

Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

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About This Item

CAS Number:

9000-95-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-569-8

MDL number:

MFCD00130542

EC Number:

3.6.1.5 (BRENDA, IUBMB)

Product Name

Apyrase from potatoes, ATPase ≥3.0 units/mg protein, lyophilized powder

biological source

potato

type

Grade I

form

lyophilized powder

ATPase activity

≥3.0 units/mg protein

composition

Protein, 30-60%

storage temp.

−20°C

Quality Level

300

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Physical form

Partially purified, lyophilized powder containing potassium succinate

Application

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+P_i → AMP+2P_i.

Other Notes

One unit will liberate 1.0 micromole of inorganic phosphorus per minute at pH 6.5 at 30 °C

Mixture of high and low ratio isoenzymes.

Packaging

Sold on the basis of ATPase units.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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The ATP Level in the mPFC Mediates the Antidepressant Effect of Calorie Restriction.

Qian Wang et al.

Neuroscience bulletin, 37(9), 1303-1313 (2021-06-06)

Food deprivation can rescue obesity and overweight-induced mood disorders, and promote mood performance in normal subjects. Animal studies and clinical research have revealed the antidepressant-like effect of calorie restriction, but little is known about the mechanism of calorie restriction-induced mood

The mode of ATP-dependent microtubule-kinesin sliding in the auxotonic condition.

I Shirakawa et al.

The Journal of experimental biology, 198(Pt 8), 1809-1815 (1995-08-01)

Kinesin is a motor protein that converts chemical energy derived from ATP hydrolysis into mechanical work to transport cellular components along microtubules. We studied the properties of ATP-dependent microtubule-kinesin sliding with two different in vitro assay systems. In one assay

Non-radioactive Assay to Determine Product Profile of Short-chain Isoprenyl Diphosphate Synthases.

Avanish Rai et al.

Bio-protocol, 11(1), e3874-e3874 (2021-03-19)

Isoprenoids represent the largest class of metabolites with amazing diversities in structure and function. They are involved in protecting plants against pathogens or herbivores or involved in attracting pollinators. Isoprenoids are derived from geranyl diphosphate (GPP; C10), farnesyl diphosphate (FPP;

Polymerization of the backbone of the pectic polysaccharide rhamnogalacturonan I.

Robert A Amos et al.

Nature plants, 8(11), 1289-1303 (2022-11-11)

Rhamnogalacturonan I (RG-I) is a major plant cell wall pectic polysaccharide defined by its repeating disaccharide backbone structure of [4)-α-D-GalA-(1,2)-α-L-Rha-(1,]. A family of RG-I:Rhamnosyltransferases (RRT) has previously been identified, but synthesis of the RG-I backbone has not been demonstrated in

ATP keeps exocytosis sites in a primed state but is not required for membrane fusion: an analysis with Paramecium cells in vivo and in vitro.

J Vilmart-Seuwen et al.

The Journal of cell biology, 103(4), 1279-1288 (1986-10-01)

We have tried to specify a widespread hypothesis on the requirement of ATP for exocytosis (membrane fusion). With Paramecium tetraurelia cells, synchronously (approximately 1 s) exocytosing trichocysts, ATP pools have been measured in different strains, including wild type cells, "non-discharge"

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