A7646

Apyrase from potatoes

Name: Apyrase from potatoes
Brand: SIGMA

ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

Synonym(s):

Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

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About This Item

CAS Number:

9000-95-7

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-569-8

MDL number:

MFCD00130542

EC Number:

3.6.1.5 (BRENDA, IUBMB)

Product Name

Apyrase from potatoes, ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

biological source

potato

type

Grade III

form

lyophilized powder (partially purified)

ATPase activity

≥60 units/mg protein

mol wt

~49 kDa by gel filtration

composition

Protein, 30-60%

solubility

H₂O: soluble 10 mg/mL, slightly hazy

application(s)

diagnostic assay manufacturing

foreign activity

Acid Phosphatase ≤5% of base activity

storage temp.

−20°C

Quality Level

200

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Analysis Note

Contains ≤ 5% acid phosphatase.

Application

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+P_i → AMP+2P_i.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.

Other Notes

Mixture of high and low ratio isoenzymes.

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Packaging

Sold on the basis of ATPase units

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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