SRP5284
HPK1, active, GST tagged human
PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
MAP4K1
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Product Name
HPK1, active, GST tagged human, PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO®
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
106-166 nmol/min·mg
mol wt
~65 kDa
NCBI accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MAP4K1(11184)
Biochem/physiol Actions
Hematopoietic progenitor kinase 1 (HPK1) acts as a tissue-specific upstream activator for the stress-activated protein kinases (SAPK)/Jun amino-terminal kinases (JNK) signaling pathway. By phosphorylating SH2 domain-containing leukocyte protein of 76 kDa (SLP-76), HPK1 effectively blocks the T cell receptor (TCR) signaling. HPK1 is implicated in the inflammatory response and in the pathogenesis of systemic lupus erythematosus (SLE). It mediates the degradation of IκB and nuclear factor-κB (NF-κB) activation by activating inhibitor of nuclear factor-κB (IκB) kinase (IKK) complex.
General description
Hematopoietic progenitor kinase 1 (HPK1) belongs to the Ste20 serine/threonine kinase superfamily. It comprises a C-terminal citron homology (CNH) domain and a total of 13 tyrosines as potential phosphorylation residues. The HPK1 gene is mapped to human chromosome 19q13.
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Preparation Note
After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Shantel Weinsheimer et al.
Physiological genomics, 32(1), 45-57 (2007-09-20)
Intracranial aneurysm (IA) is a complex genetic disease for which, to date, 10 loci have been identified by linkage. Identification of the risk-conferring genes in the loci has proven difficult, since the regions often contain several hundreds of genes. An
Qing Zhang et al.
Molecular medicine reports, 16(5), 6472-6482 (2017-09-14)
Hematopoietic progenitor kinase 1 (HPK1), also known as mitogen‑activated protein kinase kinase kinase kinase 1 is a serine/threonine protein kinase. It is involved in various cellular events, including mitogen‑activated protein kinase signaling, nuclear factor‑κB signaling, cytokine signaling, cellular proliferation and
Hua Wang et al.
The Journal of biological chemistry, 289(7), 4009-4017 (2013-12-24)
HPK1, a member of mammalian Ste20-like serine/threonine kinases, is lost in >95% pancreatic cancer through proteasome-mediated degradation. However, the mechanism of HPK1 loss has not been defined. The aims of this study are to identify the ubiquitin ligase and to
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