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219361 Anti-Cathepsin D Rabbit pAb

Anti-Cathepsin D, rabbit polyclonal, recognizes the ~50 kDa latent and the ~30 kDa active forms in breast carcinoma cell line. It is validated for ELISA, Western blotting, and immunoelectrophoresis.

Anti-Cathepsin D Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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219361
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Pricing & Availability

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219361-1MLCN
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      		 Plastic ampoule 1 ml
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      Description
      OverviewRecognizes the ~50 kDa latent and the ~30 kDa active forms of cathepsin D in breast carcinoma cell line.
      Catalogue Number219361
      Brand Family Calbiochem®
      Application Data
      Detection of human cathepsin D by immunoblotting. Samples: Low molecular weight markers (lanes 5a and 5b), cathepsin D protein (0.075 µg) (lanes 1 and 9), cathepsin D protein (0.15 µg) (lanes 2 and 8), and cathepsin D protein (0.3 µg) (lanes 3 and 7), and liver extracts (lanes 4 and 6). With primary antibody dilution 1:4000 (lanes 1-5a) and 1:2000 (lanes 5b-9). Primary antibody: Anti-Cathepsin D Rabbit pAb (Cat. No. 219361) (1:4000). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlHuman liver tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoelectrophoresis
      Application NotesELISA (≥1:1000)
      Immunoelectrophoresis (see comments)
      Application CommentsMonospecific for cathepsin D as determined by a single arc by immunoelectrophoresis against crude live extract and purified cathepsin D. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenpurified human liver cathepsin D
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      219361-1MLCN 04055977202199

      Documentation

      Anti-Cathepsin D Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cathepsin D Rabbit pAb Certificates of Analysis

      TitleLot Number
      219361

      Brochure

      Title
      Cathepsins and Related Products Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      ApplicationELISA (≥1:1000)
      Immunoelectrophoresis (see comments)
      Application Data
      Detection of human cathepsin D by immunoblotting. Samples: Low molecular weight markers (lanes 5a and 5b), cathepsin D protein (0.075 µg) (lanes 1 and 9), cathepsin D protein (0.15 µg) (lanes 2 and 8), and cathepsin D protein (0.3 µg) (lanes 3 and 7), and liver extracts (lanes 4 and 6). With primary antibody dilution 1:4000 (lanes 1-5a) and 1:2000 (lanes 5b-9). Primary antibody: Anti-Cathepsin D Rabbit pAb (Cat. No. 219361) (1:4000). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes the ~50 kDa latent and the ~30 kDa active forms of cathepsin D.
      HostRabbit
      Immunogen speciesHuman
      Immunogenpurified human liver cathepsin D
      IsotypeIgG
      Specieshuman
      Positive controlHuman liver tissue
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsMonospecific for cathepsin D as determined by a single arc by immunoelectrophoresis against crude live extract and purified cathepsin D. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Standard Handling