AB5539 Anti-Neurofilament H Antibody

AB5539
50 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, Po, R, FeICC, IHC, IH(P), WBChPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5539
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurofilament H Antibody
      Alternate Names
      • NF-H
      References
      Product Information
      FormatPurified
      Control
      • Positive control: brain.

        Negative control: kidney, liver.
      PresentationPurified chicken IgY in PBS containing 5mM sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Neurofilament H Antibody is an antibody against Neurofilament for use in IC, IH, IH(P) & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern blot: 1:1,000-1:10,000 using ECL. Recognizes a band of 200-220 kDa.

      Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.

      Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPurified bovine NF-H.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostChicken
      SpecificityRecognizes Neurofilament-Heavy (NF-H). The antibody stains sharply defined cytoplasmic filaments in neuronal cell bodies and especially axons.
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Pig
      • Rat
      • Feline
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFH
      • NF-H
      • NFH
      • KIAA0845
      Purification MethodAmmonium Sulfate Precipitation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
      SIZE: 1026 amino acids; 112480 Da
      PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
      SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.
      Molecular Weight200 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Neurofilament H Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Neurofilament H Antibody Certificates of Analysis

      TitleLot Number
      Anti-Neurofilament, Heavy2462730
      Anti-Neurofilament, Heavy - 21224192122419
      Anti-Neurofilament, Heavy - 23830702383070
      Anti-Neurofilament, Heavy - 24246402424640
      Anti-Neurofilament, Heavy - 20036482003648
      Anti-Neurofilament, Heavy - 20438862043886
      Anti-Neurofilament, Heavy - 21793002179300
      Anti-Neurofilament, Heavy - 22193292219329
      Anti-Neurofilament, Heavy - 22967022296702
      Anti-Neurofilament, Heavy - 24939312493931

      References

      Reference overviewApplicationSpeciesPub Med ID
      Activation of TRESK channels by the inflammatory mediator lysophosphatidic acid balances nociceptive signalling.
      Kollert, S; Dombert, B; Döring, F; Wischmeyer, E
      Scientific reports  5  12548  2015

      Show Abstract
      26224542 26224542
      Modeling pain in vitro using nociceptor neurons reprogrammed from fibroblasts.
      Wainger, BJ; Buttermore, ED; Oliveira, JT; Mellin, C; Lee, S; Saber, WA; Wang, AJ; Ichida, JK; Chiu, IM; Barrett, L; Huebner, EA; Bilgin, C; Tsujimoto, N; Brenneis, C; Kapur, K; Rubin, LL; Eggan, K; Woolf, CJ
      Nature neuroscience  18  17-24  2015

      Show Abstract
      25420066 25420066
      Non-aggregating tau phosphorylation by cyclin-dependent kinase 5 contributes to motor neuron degeneration in spinal muscular atrophy.
      Miller, N; Feng, Z; Edens, BM; Yang, B; Shi, H; Sze, CC; Hong, BT; Su, SC; Cantu, JA; Topczewski, J; Crawford, TO; Ko, CP; Sumner, CJ; Ma, L; Ma, YC
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  6038-50  2015

      Show Abstract
      25878277 25878277
      Computation identifies structural features that govern neuronal firing properties in slowly adapting touch receptors.
      Lesniak, DR; Marshall, KL; Wellnitz, SA; Jenkins, BA; Baba, Y; Rasband, MN; Gerling, GJ; Lumpkin, EA
      eLife  3  e01488  2014

      Show Abstract
      24448409 24448409
      Presynaptic localization of Smn and hnRNP R in axon terminals of embryonic and postnatal mouse motoneurons.
      Dombert, B; Sivadasan, R; Simon, CM; Jablonka, S; Sendtner, M
      PloS one  9  e110846  2014

      Show Abstract
      25338097 25338097
      A phenotypic culture system for the molecular analysis of CNS myelination in the spinal cord.
      Davis, H; Gonzalez, M; Stancescu, M; Love, R; Hickman, JJ; Lambert, S
      Biomaterials  35  8840-5  2014

      Show Abstract
      25064806 25064806
      Transcriptional profiling at whole population and single cell levels reveals somatosensory neuron molecular diversity.
      Chiu, IM; Barrett, LB; Williams, EK; Strochlic, DE; Lee, S; Weyer, AD; Lou, S; Bryman, GS; Roberson, DP; Ghasemlou, N; Piccoli, C; Ahat, E; Wang, V; Cobos, EJ; Stucky, CL; Ma, Q; Liberles, SD; Woolf, CJ
      eLife  3  2014

      Show Abstract
      25525749 25525749
      A Gata3-Mafb transcriptional network directs post-synaptic differentiation in synapses specialized for hearing.
      Yu, WM; Appler, JM; Kim, YH; Nishitani, AM; Holt, JR; Goodrich, LV
      eLife  2  e01341  2013

      Show Abstract
      24327562 24327562
      An in vitro model of developmental synaptogenesis using cocultures of human neural progenitors and cochlear explants.
      Nayagam, BA; Edge, AS; Needham, K; Hyakumura, T; Leung, J; Nayagam, DA; Dottori, M
      Stem cells and development  22  901-12  2013

      Show Abstract
      23078657 23078657
      The use of poly(N-[2-hydroxypropyl]-methacrylamide) hydrogel to repair a T10 spinal cord hemisection in rat: a behavioural, electrophysiological and anatomical examination.
      Pertici, V; Amendola, J; Laurin, J; Gigmes, D; Madaschi, L; Carelli, S; Marqueste, T; Gorio, A; Decherchi, P
      ASN neuro  5  149-66  2013

      Show Abstract
      23614684 23614684

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