AB3096 Anti-Orexin Antibody, Prepro

AB3096
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RELISA, IHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3096
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Orexin Antibody, Prepro
      References
      Product Information
      FormatAffinity Purified
      PresentationLiquid in PBS with 0.1% BSA.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Orexin Antibody, Prepro is an antibody against Orexin for use in ELISA, IH & WB.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1-10 μg/mL using Chemiluminescence technique

      ELISA: 1:10,000 - 100,000

      Immunohistochemistry: 2-20 μg/mL.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenA 17 mer peptide (designated PPOXA11-A) mapping near the C-terminus of mouse prepro-Orexin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityMouse Prepro-Orexin. Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a hypothalamic neuropeptide precusor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.
      Gene Symbol
      • HCRT
      • Hypocretin
      • OX
      • orexin
      • Hcrt
      • PPOX
      • PPORX
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O43612 # Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.
      SIZE: 131 amino acids; 13363 Da
      SUBCELLULAR LOCATION: Rough endoplasmic reticulum (By similarity). Cytoplasmic vesicle (By similarity). Cell junction, synapse (By similarity). Note=Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses (By similarity).
      TISSUE SPECIFICITY: Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
      PTM: Specific enzymatic cleavages at paired basic residues yield the different active peptides.
      DISEASE: SwissProt: O43612 # Defects in HCRT are a cause of narcolepsy [MIM:161400]. Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed.
      SIMILARITY: SwissProt: O43612 ## Belongs to the orexin family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsCan be refrigerated at 2-8°C in undiluted aliquots for short term use. Maintain frozen at -20°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Orexin Antibody, Prepro SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Orexin Antibody, Prepro Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-PREPRO-OREXIN - 32139613213961
      RABBIT ANTI-PREPRO-OREXIN - 33799713379971
      RABBIT ANTI-PREPRO-OREXIN AFFINITY PURIFIED3164182
      RABBIT ANTI-PREPRO-OREXIN AFFINITY PURIFIED POLYCLONAL ANTIBODY - 23764082376408
      RABBIT ANTI-PREPRO-OREXIN AFFINITY PURIFIED -26109662610966
      RABBIT ANTI-PREPRO-OREXIN AFFINITY PURIFIED -27183202718320
      RABBIT ANTI-PREPRO-OREXIN AFFINITY PURIFIED -27492222749222
      RABBIT ANTI-PREPRO-OREXIN AFFINITY PURIFIED -27623792762379
      RABBIT ANTI-PREPRO-OREXIN AFFINITY PURIFIED -27805862780586
      RABBIT ANTI-PREPRO-OREXIN AFFINITY PURIFIED -28070232807023

      References

      Reference overviewSpeciesPub Med ID
      The orexin system in the enteric nervous system of the bottlenose dolphin (Tursiops truncatus).
      Gatta, C; Russo, F; Russolillo, MG; Varricchio, E; Paolucci, M; Castaldo, L; Lucini, C; de Girolamo, P; Cozzi, B; Maruccio, L
      PloS one  9  e105009  2014

      Show Abstract
      25144456 25144456
      Mu-opioid stimulation in rat prefrontal cortex engages hypothalamic orexin/hypocretin-containing neurons, and reveals dissociable roles of nucleus accumbens and hypothalamus in cortically driven feeding.
      Mena, JD; Selleck, RA; Baldo, BA
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  18540-52  2013

      Show Abstract
      24259576 24259576
      Exaggerated natriuresis during clamping of systemic NO supply in healthy young men.
      Simonsen, JA; Rasmussen, MS; Vach, W; Høilund-Carlsen, PF; Bie, P
      Clinical science (London, England : 1979)  122  63-73  2012

      Show Abstract
      21749320 21749320
      Disorganized epithelial polarity and excess trophectoderm cell fate in preimplantation embryos lacking E-cadherin.
      Stephenson RO, Yamanaka Y, Rossant J
      Development  137  3383-91. Epub 2010 Sep 8.  2010

      Show Abstract
      20826529 20826529
      A single postnatal injection of oxytocin rescues the lethal feeding behaviour in mouse newborns deficient for the imprinted Magel2 gene.
      Schaller, F; Watrin, F; Sturny, R; Massacrier, A; Szepetowski, P; Muscatelli, F
      Human molecular genetics  19  4895-905  2010

      Show Abstract
      20876615 20876615
      Afferents to the GABAergic tail of the ventral tegmental area in the rat.
      Kaufling, Jennifer, et al.
      J. Comp. Neurol., 513: 597-621 (2009)  2009

      Show Abstract
      Rat19235223 19235223
      Expression of orexin A and its receptor 1 in the choroid plexuses from buffalo brain.
      Simona Tafuri, Luigi M Pavone, Vincenzo Mastellone, Anna Spina, Luigi Avallone, Alfredo Vittoria, Norma Staiano, Gaetano Scala, Simona Tafuri, Luigi M Pavone, Vincenzo Mastellone, Anna Spina, Luigi Avallone, Alfredo Vittoria, Norma Staiano, Gaetano Scala, Simona Tafuri, Luigi M Pavone, Vincenzo Mastellone, Anna Spina, Luigi Avallone, Alfredo Vittoria, Norma Staiano, Gaetano Scala
      Neuropeptides  43  73-80  2009

      Show Abstract
      19250669 19250669
      Differential c-Fos immunoreactivity in arousal-promoting cell groups following systemic administration of caffeine in rats.
      Samüel Deurveilher, Henry Lo, Jeremy A Murphy, Joan Burns, Kazue Semba
      The Journal of comparative neurology  498  667-89  2006

      Show Abstract
      16917819 16917819
      Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior.
      Sakurai, T, et al.
      Cell, 92: 573-85 (1998)  1998

      Show Abstract
      9491897 9491897

      Data Sheet

      Title
      RABBIT ANTI-PREPRO-OREXIN AFFINITY PURIFIED

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies