517310 Anti-P-Glycoprotein Mouse mAb (C219)

517310
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Ht, H, M, RMMonoclonal Antibody

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
517310-500ULCN
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      Glass bottle 500 ul
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      Description
      OverviewRecognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
      Catalogue Number517310
      Brand Family Calbiochem®
      References
      ReferencesBeck, W.T., et al. 1996. Cancer Res. 56, 3010.
      Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
      Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
      Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
      Product Information
      DeclarationImmunoblotting Lee, G., et al. 2001. J. Pharmacol. Exp. Ther. 299, 204.
      FormLiquid
      FormulationIn PBS, 1% BSA.
      Positive controlCell lines or frozen tissue, such as liver or colon
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Ledoux, S., et al. 2003. Cancer Res. 63, 7284. Veau, C., et al. 2002. J. Pharmacol. Exp. Ther. 302, 742. Hosoyo, K., et al. 2000. AAPS PharmSci. 2, E27.
      Key Applications Immunocytochemistry
      Immunoprecipitation
      Paraffin Sections
      Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesFlow Cytometry (5-10 µg/1 x 10⁶ cells)
      Frozen Sections (5-10 µg/ml)
      Immunoblotting (1-10 µg/ml, see application references, see comments)
      Immunocytochemistry (see comments)
      Immunoprecipitation (see comments)
      Paraffin Sections (1-10 µg/ml)
      Application CommentsAlso recognizes ~150 kDa sister P-glycoprotein (SPGP). Frozen sections should be fixed in acetone for 10 min at -20°C. Formalin-fixed, paraffin tissue sections should be deparaffinized and rehydrated by conventional means. Boiling samples may reduce visibility of the protein by immunoblotting (see application references). This antibody is also reported to work for immunocytochemistry and immunoprecipitation. A ~200 kDa protein that migrates in the same position as myosin is also seen by immunoblotting. Variables associated with assay conditions will dictate the proper working dilutions.
      Biological Information
      ImmunogenSDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a multidrug resistant human cell line
      ImmunogenHamster and Human
      CloneC219
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Hamster
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-P-Glycoprotein Mouse mAb (C219) Certificates of Analysis

      TitleLot Number
      517310

      References

      Reference overview
      Beck, W.T., et al. 1996. Cancer Res. 56, 3010.
      Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
      Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
      Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.

      Citations

      Title
    • Zhigang Zhang, Jin-Ying Wu, William Hait and Jin-Ming Yang. (2004) Regulation of the stability of P-glycoprotein by ubiquitination.. Molecular Pharmacology 66, 395-403.
    • Severine Ledoux, et al. (2003) Glucose depletion enhances P-glycoprotein expression in hepatoma cells: role of endoplasmic reticulum stress response. Cancer Research 63, 7284-7290.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-June-2013 JSW
      ApplicationFlow Cytometry (5-10 µg/1 x 10⁶ cells)
      Frozen Sections (5-10 µg/ml)
      Immunoblotting (1-10 µg/ml, see application references, see comments)
      Immunocytochemistry (see comments)
      Immunoprecipitation (see comments)
      Paraffin Sections (1-10 µg/ml)
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
      BackgroundP-glycoprotein is typically expressed in cells with secretory and excretory functions. Its highest expression is in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P-glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P-glycoprotein have also been reported in multidrug-resistant cell lines and in various tumors, sarcomas and lymphomas.
      HostMouse
      Immunogen speciesHamster and Human
      ImmunogenSDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a multidrug resistant human cell line
      CloneC219
      IsotypeIgG₁
      Specieshamster, human, mouse, rat
      Positive controlCell lines or frozen tissue, such as liver or colon
      FormLiquid
      FormulationIn PBS, 1% BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAlso recognizes ~150 kDa sister P-glycoprotein (SPGP). Frozen sections should be fixed in acetone for 10 min at -20°C. Formalin-fixed, paraffin tissue sections should be deparaffinized and rehydrated by conventional means. Boiling samples may reduce visibility of the protein by immunoblotting (see application references). This antibody is also reported to work for immunocytochemistry and immunoprecipitation. A ~200 kDa protein that migrates in the same position as myosin is also seen by immunoblotting. Variables associated with assay conditions will dictate the proper working dilutions.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBeck, W.T., et al. 1996. Cancer Res. 56, 3010.
      Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
      Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
      Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
      Citation
    • Zhigang Zhang, Jin-Ying Wu, William Hait and Jin-Ming Yang. (2004) Regulation of the stability of P-glycoprotein by ubiquitination.. Molecular Pharmacology 66, 395-403.
    • Severine Ledoux, et al. (2003) Glucose depletion enhances P-glycoprotein expression in hepatoma cells: role of endoplasmic reticulum stress response. Cancer Research 63, 7284-7290.
    • Application referencesImmunoblotting Ledoux, S., et al. 2003. Cancer Res. 63, 7284. Veau, C., et al. 2002. J. Pharmacol. Exp. Ther. 302, 742. Hosoyo, K., et al. 2000. AAPS PharmSci. 2, E27.