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NA29 Anti-p16 (Ab-1) Mouse mAb (DCS-50.1/H4)

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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NA29-100UGCN
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~16 kDa p16 protein in Saos-2 cells and bladder tissue. Sold under license of U.S. Patent 6,482,929. Sold under license of U.S. Patent 5,843,756, 6,090,578, and corresponding patents.
      Catalogue NumberNA29
      Brand Family Calbiochem®
      References
      ReferencesKoh, J., et al. 1995. Nature 375, 506.
      Lukas, J., et al. 1995. Nature 375, 503.
      Cheng, J.Q., et al. 1994. Cancer Res. 54, 5547.
      Jen, J., et al. 1994. Cancer Res. 54, 6353.
      Kamb, A., et al. 1994 Science 264, 436.
      Nobori, T., Miura, K., et al. 1994. Nature 368, 753.
      Okamoto, A., Demetrick, D.J., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 11045.
      Serrano, M., et al. 1993. Nature 366 704.
      Product Information
      DeclarationSold under license of U.S. Patents 5,843,756, 6,090,578 and corresponding patents.
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin pH 7.5.
      Negative controlU2OS cells
      Positive controlSaos-2 cells or bladder tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Kang, M.K., et al. 2004. J. Cell. Physiol. 199, 364.
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (0.5-5 µg/ml, see application references)
      Application CommentsDoes not cross-react with the closely related p15 protein. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human p16
      ImmunogenHuman
      CloneDCS-50.1/H4
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      NA29-100UGCN 04055977227758

      Documentation

      Anti-p16 (Ab-1) Mouse mAb (DCS-50.1/H4) Certificates of Analysis

      TitleLot Number
      NA29

      References

      Reference overview
      Koh, J., et al. 1995. Nature 375, 506.
      Lukas, J., et al. 1995. Nature 375, 503.
      Cheng, J.Q., et al. 1994. Cancer Res. 54, 5547.
      Jen, J., et al. 1994. Cancer Res. 54, 6353.
      Kamb, A., et al. 1994 Science 264, 436.
      Nobori, T., Miura, K., et al. 1994. Nature 368, 753.
      Okamoto, A., Demetrick, D.J., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 11045.
      Serrano, M., et al. 1993. Nature 366 704.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      ApplicationImmunoblotting (0.5-5 µg/ml, see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-2 mouse myeloma cells. Recognizes the ~16 kDa p16 protein.
      BackgroundVarious cyclin dependent kinases (CDKs) are critical for progression through the G1 phase of the cell cycle, and the enzymatic activity of CDKs is dependent upon specific association with G1 cyclins. The active cyclin/CDK complex can be inhibited by a number of different inhibitor proteins including the p16 product of the Multiple Tumor Suppressor 1 gene (MTS1). p16 was initially identified as a component of cyclin/CDK complexes and shown to specifically inhibit CDK4, thus earning the name p16INK4. Independent studies have demonstrated that the gene encoding p16 is commonly deleted in many different tumor cell lines. Recent evidence implicates the p16 protein in the Retinoblastoma pathway for control of cell growth and proliferation and demonstrates that p16 can function as a tumor suppressor protein to G1-arrest cells. p16 is also known as p16MTS1, and p16CDK4I. The gene maps to human chromosome 9p21 and has been named MTS1 or CDKN2. Although the role of the CDK inhibitor p16 as a cell cycle regulator is well established, other aspects of p16 function and the issue of alterations in p16 as a primary event in tumorigenesis are still under investigation.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human p16
      CloneDCS-50.1/H4
      IsotypeIgG₁
      Specieshuman
      Positive controlSaos-2 cells or bladder tissue
      Negative controlU2OS cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with the closely related p15 protein. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesKoh, J., et al. 1995. Nature 375, 506.
      Lukas, J., et al. 1995. Nature 375, 503.
      Cheng, J.Q., et al. 1994. Cancer Res. 54, 5547.
      Jen, J., et al. 1994. Cancer Res. 54, 6353.
      Kamb, A., et al. 1994 Science 264, 436.
      Nobori, T., Miura, K., et al. 1994. Nature 368, 753.
      Okamoto, A., Demetrick, D.J., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 11045.
      Serrano, M., et al. 1993. Nature 366 704.
      Application referencesImmunoblotting Kang, M.K., et al. 2004. J. Cell. Physiol. 199, 364.