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220551 Chloramphenicol - CAS 56-75-7 - Calbiochem

220551
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₁₁H₁₂Cl₂N₂O₅56-75-7

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220551-25GMCN
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      Plastic ampoule 25 gm
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      Description
      OverviewSynthetic bacteriostatic antibiotic that inhibits the translation of RNA by blocking the peptidyltransferase reaction on ribosomes.
      Catalogue Number220551
      Brand Family Calbiochem®
      References
      ReferencesLech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1, (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
      Lu, J., and Jiang, C. 1993. Biochem. Biophys. Res. Commun. 196, 12.
      Saltarelli, M.J., et al. 1993. Virol. 197, 35.
      Holt, J.T. 1992. Ann. N.Y. Acad. Sci. 660, 88.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, NY, p. 1.6, A.6.
      Product Information
      CAS number56-75-7
      FormGrayish to off-white crystalline powder
      Hill FormulaC₁₁H₁₂Cl₂N₂O₅
      Chemical formulaC₁₁H₁₂Cl₂N₂O₅
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityPotency: ≥970 µg/mg
      Primary Targettrasnslation of RNA
      Purity≥97% by Assay
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSAB6825000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Chloramphenicol - CAS 56-75-7 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Chloramphenicol - CAS 56-75-7 - Calbiochem Certificates of Analysis

      TitleLot Number
      220551

      References

      Reference overview
      Lech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1, (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
      Lu, J., and Jiang, C. 1993. Biochem. Biophys. Res. Commun. 196, 12.
      Saltarelli, M.J., et al. 1993. Virol. 197, 35.
      Holt, J.T. 1992. Ann. N.Y. Acad. Sci. 660, 88.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, NY, p. 1.6, A.6.

      Brochure

      Title
      Antibiotics Profiler
      Bulk Product Guide
      Overnight Express Autoinduction Systems
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-January-2014 JSW
      DescriptionSynthetic bacteriostatic antibiotic that inhibits the translation of RNA to protein by binding to the 50S ribosomal subunit, blocking the peptidyl transferase reaction. Chloramphenicol resistance is encoded by the resistance gene cat, whose product, chloramphenicol acetyltransferase, inactivates the antibiotic via acetylation. Typically used at 30-50 µg/ml for selection of chloramphenicol-resistant Escherichia coli.
      FormGrayish to off-white crystalline powder
      CAS number56-75-7
      RTECSAB6825000
      Chemical formulaC₁₁H₁₂Cl₂N₂O₅
      Structure formulaStructure formula
      Purity≥97% by Assay
      Biological activityPotency: ≥970 µg/mg
      SolubilityEthanol, Methanol (35 mg/ml), or H₂O (2.5 mg/ml)
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.
      Merck USA index14 2077
      ReferencesLech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1, (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
      Lu, J., and Jiang, C. 1993. Biochem. Biophys. Res. Commun. 196, 12.
      Saltarelli, M.J., et al. 1993. Virol. 197, 35.
      Holt, J.T. 1992. Ann. N.Y. Acad. Sci. 660, 88.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, NY, p. 1.6, A.6.

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      Categories

      Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Other Inhibitors of Biological Interest > Protein Synthesis Inhibitors
      Life Science Research > Inhibitors and Biochemicals > Biochemicals > Antibiotics > Antimicrobial Agents
      Life Science Research > Cell Culture and Systems > Antibiotics & Selection Agents