SCC008 ReNcell® VM Human Neural Progenitor Cell LIne

SCC008
1 ea  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCC008
      Brand Family Chemicon®
      Trade Name
      • ReNcell
      • Chemicon
      DescriptionReNcell® VM Human Neural Progenitor Cell LIne
      OverviewReNcell® VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell® VM was derived from the ventral mesencephalon region of human fetal brain. Immortalized by retroviral transduction with the v-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell® VM retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell® VM was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. In experiments performed by the ReNeuron Group plc, ReNcell® VM can be differentiated in vitro to a high level of human dopaminergic neurons. Neurons differentiated from ReNcell® VM have furthermore been shown to be electophysiologically active. ReNcell® VM may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell® VM cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures.

      ReNcell Now Used in a 3D Cell Model of Alzheimer's Disease!
      Tanzi, R and Kim, DY et. al. A three-dimensional human neural cell culture model of Alzheimer's disease. Nature. 2014 Nov 13;515(7526):274-8.
      (Published in Nature)
      Materials Required but Not Delivered1. ReNcell® NSC Maintenance Medium (Millipore Cat. No. SCM005)
      2. ReNcell® NSC Freezing Medium (Millipore Cat. No. SCM007)
      3. Basic fibroblast growth factor (bFGF; FGF-2; Specific Activity > 2 X 106 Units/mg. Millipore Cat. No. GF003)
      4. Epidermal growth factor (EGF; Specific Activity > 1 x 107 Units/mg; MilliporeCat. No. GF001)
      5. Laminin (Millipore Cat. No. CC095)
      6. DMEM/F12 w/o HEPES, w/ L-Glutamine (Millipore Cat. No. DF-042-B)
      7. Accutase (Millipore Cat. No. SCR005)
      8. Tissue culture-ware
      9. Phosphate-Buffered Saline (1X PBS) (Millipore Cat. No. BSS-1005-B)
      10. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)
      11. Blocking Solution (5% normal donkey serum, 0.3% Triton X-100 in 1X PBS)
      12. Primary and secondary antibodies
      13. 4'-6-Diamidino-2-phenylindole (DAPI) / PBS solution
      14. Anti-fading mounting solution (DABCO/PVA)
      15. Hemacytometer
      16. Microscope
      References
      Product Information
      Components
      • > 1x106 viable ReNcell® VM cells derived from human ventral mesencephalon brain tissue, cryopreserved. Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationReNcell® VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.
      Key Applications
      • Stem Cell Culture
      Biological Information
      SourceHuman ventral mesencephalon brain tissue
      Stem Cell Type
      • Neural Stem Cells
      Cell Line Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach lot of ReNcell® VM cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities. Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWhen stored at the recommended storage conditions (liquid nitrogen), ReNcell® VM cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.
      Packaging Information
      Material Size1 ea
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ReNcell® VM Human Neural Progenitor Cell LIne SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewApplicationPub Med ID
      A dynamic view of the proteomic landscape during differentiation of ReNcell VM cells, an immortalized human neural progenitor line.
      Song, Y; Subramanian, K; Berberich, MJ; Rodriguez, S; Latorre, IJ; Luria, CM; Everley, R; Albers, MW; Mitchison, TJ; Sorger, PK
      Sci Data  6  190016  2019

      Show Abstract
      30778261 30778261
      A three-dimensional human neural cell culture model of Alzheimer's disease.
      Choi, SH; Kim, YH; Hebisch, M; Sliwinski, C; Lee, S; D'Avanzo, C; Chen, H; Hooli, B; Asselin, C; Muffat, J; Klee, JB; Zhang, C; Wainger, BJ; Peitz, M; Kovacs, DM; Woolf, CJ; Wagner, SL; Tanzi, RE; Kim, DY
      Nature  2014

      Show Abstract
      25307057 25307057
      Dynamic mass redistribution assay decodes differentiation of a neural progenitor stem cell.
      Pai, S; Verrier, F; Sun, H; Hu, H; Ferrie, AM; Eshraghi, A; Fang, Y
      Journal of biomolecular screening  17  1180-91  2012

      Show Abstract
      22885730 22885730
      Interplay between REST and nucleolin transcription factors: a key mechanism in the overexpression of genes upon increased phosphorylation.
      Tediose, Teeo, et al.
      Nucleic acids research, (2010)  2010

      Show Abstract
      20100803 20100803
      Transcriptional signature and memory retention of human-induced pluripotent stem cells.
      Marchetto, Maria C N, et al.
      PLoS ONE, 4: e7076 (2009)  2009

      Show Abstract
      19763270 19763270
      Differential development of neuronal physiological responsiveness in two human neural stem cell lines.
      Donato, R; Miljan, EA; Hines, SJ; Aouabdi, S; Pollock, K; Patel, S; Edwards, FA; Sinden, JD
      BMC neuroscience  8  36  2007

      Show Abstract
      Immunocytochemistry17531091 17531091

      Technical Info

      Title
      Derivation of oligodendrocyte progenitor cells from a human neural stem cell line
      ReNcell VM Cell Line - A Model of Human Neural Development and Differentiation
      ReNcell® Human Neural Progenitors: Renewable and Consistent Supply of Human Functional Neurons

      Data Sheet

      Title
      ReNcell™ VM Human Neural Progenitor Cell Line

      Newsletters / Publications

      Title
      Cellutions Newsletter: 2007, Volume 1
      Cellutions Newsletter: 2008, Volume 1
      Cellutions Newsletter: 2008, Volume 3

      Related Products & Applications

      Accessories

      Catalogue Number Description  
      GF003 Fibroblast Growth Factor basic Protein, Human recombinant Show Pricing & Availability
      SCM005 ReNcell® NSC Maintenance Media Show Pricing & Availability
      SCM007 ReNcell® Neural Stem Cell Freezing Medium Show Pricing & Availability
      SCR055 ENStem Human Neural Progenitor Expansion Kit Show Pricing & Availability

      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > Stem Cell Lines