505156 SUMOylation Inhibitor III, 2-D08 - Calbiochem

505156
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₅H₁₀O₅

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      Glass bottle 10 mg
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      Description
      OverviewA cell-permeable trihydroxyflavone compound that prevents the transfer of SUMO (Cat. Nos. 662037, 662039, and 662042) from the E2 thioester to the substrate without affecting SUMO-activating enzyme E1 (SAE-1/2; Cat. Nos. 662073 and 662074) or E2 Ubc9-SUMO thioester formation. A 6 h pretreatment with 100 µM 2-D08 is shown to effectively inhibit 10 µM CPT- (Camptothecin; Cat. No. 208925) induced Topoisomerase I SUMOylation in breast cancer ZR-75-1 and BT-474 cultures without affecting overall cellular protein ubiquitinations. Anacardic and Ginkgolic Acid (Cat. No. 172050 and 345887, respectively) in comparison, both inhibit E1-SUMO thioester formation instead.
      Catalogue Number505156
      Brand Family Calbiochem®
      Synonyms2D08, SUMO E2 Ubc9 Inhibitor, 2-(2,3,4-Trihydroxyphenyl)-4H-chromen-4-one
      References
      ReferencesKim, Y.S., et al. 2013. Chem. Biol. 20, 604.
      Cotelle, N., et al. 1996. Free Radic. Biol. Med. 20, 35.
      Product Information
      FormOrange-brown powder
      Hill FormulaC₁₅H₁₀O₅
      Chemical formulaC₁₅H₁₀O₅
      ReversibleY
      Quality LevelMQ300
      Applications
      Biological Information
      Primary TargetSUMO E2-thioester complex
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SUMOylation Inhibitor III, 2-D08 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Kim, Y.S., et al. 2013. Chem. Biol. 20, 604.
      Cotelle, N., et al. 1996. Free Radic. Biol. Med. 20, 35.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision18-April-2014 JSW
      Synonyms2D08, SUMO E2 Ubc9 Inhibitor, 2-(2,3,4-Trihydroxyphenyl)-4H-chromen-4-one
      DescriptionA cell-permeable trihydroxyflavone compound that inhibits androgen receptor peptide, IκBα, and Topoisomerase I SUMOylation in cell-free assays (>90% at 30 µM) by selectively preventing the transfer of SUMO (Cat. Nos. 662037, 662039, and 662042) from the E2 thioester to the substrate without affecting SUMO-activating enzyme E1 (SAE-1/2; Cat. Nos. 662073 and 662074) or E2 Ubc9-SUMO thioester formation. A 6 h pretreatment with 100 µM 2-D08 is shown to effectively inhibit 10 µM CPT- (Camptothecin; Cat. No. 208925) induced cellular Topoisomerase I SUMOylation in breast cancer ZR-75-1 and BT-474 cultures without affecting overall cellular protein ubiquitinations. Anacardic and Ginkgolic Acid (Cat. No. 172050 and 345887, respectively) in comparison, both inhibit E1- SUMO thioester formation instead.
      FormOrange-brown powder
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₁₅H₁₀O₅
      Purity≥95% by HPLC
      SolubilityDMSO (25 mg/ml). Use only fresh DMSO for reconstitution.
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesKim, Y.S., et al. 2013. Chem. Biol. 20, 604.
      Cotelle, N., et al. 1996. Free Radic. Biol. Med. 20, 35.