Pricing & Availability
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|70055-3CN||Plastic ampoule||10 μg||
|Overview||The pBACsurf-1 transfer plasmid is designed for in-frame insertion of target genes between the gp64 signal sequence and the mature protein coding sequence (under the control of the polh promoter). Expressed fusion proteins, including glycoproteins, are secreted and incorporated onto the virion surface, anchored by the transmembrane domain of gp64 (1). Target proteins are displayed at the poles of the virion, where they appear to be localized as hetero-oligomers with the wild-type gp64 also encoded by the virus (1).
For virus display, inserts must lack an internal stop codon and maintain the appropriate open reading frame. Inserts in-frame with the N-terminal gp64 signal sequence and carrying an internal stop codon can produce target proteins that may be secreted from the recombinant virus-infected cell, provided they lack a membrane-spanning sequence. Alternatively, target proteins may be displayed on the cell surface and on budded virions if they remain in-frame with the downstream gp64 gene.
The plasmid is compatible with BacVector™-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter. The cloning/expression region of the coding strand transcribed from the polh promoter is shown on the vector map (TB148). The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the non-coding strand. Single stranded sequencing of phage-derived DNA can be performed using the gp64 signal primer.
This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: email@example.com.
|References||1. Boublik, Y., Di Bonito, P., and Jones, I.M. (1995) Bio/Technology 13, 1079–1084.|
|Safety Information according to GHS|
|Product Usage Statements|
|Storage and Shipping Information|
|Ship Code||Shipped with Blue Ice or with Dry Ice|
|Avoid freeze/thaw||Avoid freeze/thaw|
|Do not freeze||Ok to freeze|
pBAC™surf-1 DNA - Novagen SDS
|1. Boublik, Y., Di Bonito, P., and Jones, I.M. (1995) Bio/Technology 13, 1079–1084.|
|Insect Cell Expression|
|TB216 BacVector® Transfection Kits|
|TB148 pBACsurf-1 Vector Map|