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Recombinant, human ACE ectodomain (amino acids 30-1261) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. ACE is a zinc metallopeptidase that cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II. Inactivates bradykinin by the sequential removal of two C-terminal dipeptides. ACE is a type I membrane protein that exists in two isoforms, somatic and germinal. By SDS-PAGE, the apparent molecular mass of the glycosylated protein appears larger than 120 kDa under either reducing or non-reducing condition.
Catalogue Number
176871
Brand Family
Calbiochem®
Synonyms
Peptidyl-Dipetidase A, ACE
References
References
Wakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci.977, 232. Hu, J., et al. 2001. J. Biol. Chem.276, 47863. Kessler, S.P., et al. 2000. J. Biol. Chem.275, 26259. Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA.85, 9386.
Product Information
Unit of Definition
One unit is defined as the amount of enzyme that will cleave 1 nmol MCA-RPPGFSAFK(DNP)-OH per min at 25°C, pH 6.5.
Form
Liquid
Formulation
In 75 mM NaCl, 12.5 mM Tris, 500 nM ZnCl₂, 40% glycerol, pH 7.5.
Wakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci.977, 232. Hu, J., et al. 2001. J. Biol. Chem.276, 47863. Kessler, S.P., et al. 2000. J. Biol. Chem.275, 26259. Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA.85, 9386.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
14-April-2008 RFH
Synonyms
Peptidyl-Dipetidase A, ACE
Description
Recombinant, human ACE ectodomain (amino acids 30-1261) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. ACE is a zinc metallopeptidase that cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II. ACE gene polymorphism has been a recent topic in Alzheimer's disease studies. ACE is a type I membrane protein and exists in two isoforms, somatic and germinal. By SDS-PAGE, the apparent molecular weight of the glycosylated protein appears larger to be than 120 kDa under both reducing and non-reducing conditions.
Form
Liquid
Formulation
In 75 mM NaCl, 12.5 mM Tris, 500 nM ZnCl₂, 40% glycerol, pH 7.5.
Concentration Label
Please refer to vial label for lot-specific concentration
Purity
≥95% by SDS-PAGE
Contaminants
Endotoxins: ≤1.0 EU/µg ACE
Specific activity
≥1 unit/µg protein
Unit definition
One unit is defined as the amount of enzyme that will cleave 1 nmol MCA-RPPGFSAFK(DNP)-OH per min at 25°C, pH 6.5.
Storage
Avoid freeze/thaw
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Wakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci.977, 232. Hu, J., et al. 2001. J. Biol. Chem.276, 47863. Kessler, S.P., et al. 2000. J. Biol. Chem.275, 26259. Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA.85, 9386.
