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Detection of human ALOX12 by immunoblotting. Sample: Partial, recombinant, human ALOX12 protein. Primary antibody: Anti-ALOX12 Mouse mAb (2D10) (Cat. No. ST1558) (1-5 µg/ml). Detection: chemiluminescence.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids 564-664 of human ALOX12, expressed as a GST fusion protein
Clone
2D10
Host
Mouse
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-November-2010 RFH
Synonyms
Anti-Arachidonate 12-Lipoxygenase
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of human ALOX12 by immunoblotting. Sample: Partial, recombinant, human ALOX12 protein. Primary antibody: Anti-ALOX12 Mouse mAb (2D10) (Cat. No. ST1558) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the ALOX12 protein.
Background
Lipoxygenases (LOXs) are a family of non-heme iron dioxygenases that are involved in the production and metabolism of fatty acid hydroperoxidases. There are six lipoxygenase isozymes; 5-LOX, 12R-LOX, 12S-LOX, 15-LOX-1 and 15-LOX-2, which catalyze the regio-selective addition of oxygen into arachidonic acid, and epidermal LOX (E-LOX) which metabolizes 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE) to a specific epoxyalcohol compound. Different isozymes have different physiological roles; 5-, 12R-, 12S- and 15-LOX-1 have proliferative, antiapoptotic and angiogenic functions whilst 15-LOX-2 has roles in differentiation, growth arrest and the induction of apoptosis. E-LOX, the most divergent member of this family, is involved in terminal differentiation of keratinocytes and has a major role in skin barrier function. Perturbations in lipoxygenase enzymes have been implicated in various human diseases including bronchial asthma, atherosclerosis, osteoporosis and some cancers.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids 564-664 of human ALOX12, expressed as a GST fusion protein
Clone
2D10
Isotype
IgG2a
Species
human
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
