Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Detection of human ATF3 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length, recombinant, human ATF3 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-ATF3 Mouse mAb (6B8) (Cat. No. DR1086). Detection: chemiluminescence.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
Full-length, recombinant, human ATF3 (aa 1-182), expressed as a GST fusion protein.
Clone
6B8
Host
Mouse
Isotype
IgG₃
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
04-November-2010 RFH
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of human ATF3 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length, recombinant, human ATF3 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-ATF3 Mouse mAb (6B8) (Cat. No. DR1086). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the ATF3 protein.
Background
Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (δZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.
Host
Mouse
Immunogen
Full-length, recombinant, human ATF3 (aa 1-182), expressed as a GST fusion protein.
Clone
6B8
Isotype
IgG₃
Species
human
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
