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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

CP01 Sigma-AldrichAnti-Actin (Ab-1) Mouse mAb (JLA20)

Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.

Anti-Actin (Ab-1) Mouse mAb (JLA20) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
小册子
Data Sheet
Citations

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
A Broad Range Of Species	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody. Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1 Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTIN Entrez Gene Name: actin, alpha 1, skeletal muscle Hu Entrez ID: 58, 71 (Related Antibodies: CP64) Mu Entrez ID: 11459 ;-; 11465 Rat Entrez ID: 29437 ;-; 287876
Catalogue Number	CP01
Brand Family	Calbiochem®

References
References	Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp. Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769. Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335. Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701. Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

Product Information
Form	Liquid
Formulation	100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
Positive control	All animal cell lines
Preservative	≤0.1% sodium azide (actin antibody only, CP01-100UL)
Quality Level	MQ100

Applications
Application References	Immunoblotting Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.
Key Applications	Immunoblotting (Western Blotting) Immunofluorescence
Application Notes	Immunoblotting (1:5000-1:20000) Immunofluorescence (1:10000, see application references) Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
Application Comments	Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.

Biological Information
Immunogen	chicken cytoskeletal proteins
Immunogen	Chicken
Clone	JLA20
Host	Mouse
Isotype	IgM
Species Reactivity	A Broad Range Of Species
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Multiple Toxicity Values, refer to MSDS
Storage	-20°C
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot ascites and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
CP01-1EACN	07790788053697

Documentation

Anti-Actin (Ab-1) Mouse mAb (JLA20) MSDS

职位
物料安全数据表 (MSDS)

Anti-Actin (Ab-1) Mouse mAb (JLA20) 分析证书

标题	批号
CP01	输入批号

参考

参考信息概述
Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp. Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769. Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335. Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701. Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.

小册子

标题
Antibody Sourcebookﾙ Sixth Edition

引用

标题

Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	29-October-2008 JSW
Application	Immunoblotting (1:5000-1:20000) Immunofluorescence (1:10000, see application references) Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
Description	Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
Background	Cytoskeletal proteins are found in highly organized arrays within the cytoplasm of higher eukaryotic cells. These proteins are intimately involved in functions such as cell and intracellular organelle movement, maintenance of cell shape and endocytosis. The cytoskeleton of most eukaryotic cells is comprised of at least three fibrous systems each with accessory proteins: the microfilaments, composed of actin; the microfibrils, composed of tubulin; and the intermediate filaments composed of vimentin. Monoclonal antibodies have been used to study the components of the cytoskeleton and their intracellular location, changes in the expression of these elements during differentiation, and the function of the cytoskeleton in living cells by microinjection of the antibodies.
Host	Mouse
Immunogen species	Chicken
Immunogen	chicken cytoskeletal proteins
Clone	JLA20
Isotype	IgM
Species	a broad range of species
Positive control	All animal cell lines
Form	Liquid
Formulation	100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
Preservative	≤0.1% sodium azide (actin antibody only, CP01-100UL)
Comments	Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
Storage	-20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot ascites and freeze (-20°C).
Toxicity	Multiple Toxicity Values, refer to MSDS
References	Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp. Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769. Lin, J.J. 1981. Proc. Natl. Acad. Sci. 78, 2335. Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701. Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
Citation	Wayne D. Thomas, et al. (2000) TNF-Related Apoptosis-Inducing Ligand-Induced Apoptosis of Melanoma is Associated with Changes in Mitochondrial Membrane Potential and Perinuclear Clustering of Mitochondria. Journal of Immunology 165, 5612-5620.
Application references	Immunoblotting Thomas, W.D., et al. 2000. J. Immunol. 165, 5612.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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