数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABS501 Sigma-AldrichAnti-Anoctamin-5 Antibody, clone 5F7

This Anti-Anoctamin-5 Antibody, clone 5F7 is validated for use in Western Blotting for the detection of Anoctamin-5.

Anti-Anoctamin-5 Antibody, clone 5F7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
小册子
技术信息
Posters

Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: ANO5, GDD1, TMEM16E UniProt Number: Q75V66

View Products on Sigmaaldrich.com

Recommended Products

概述

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H	WB	M	Unpurified	Monoclonal Antibody

Description
Catalogue Number	MABS501
Description	Anti-Anoctamin-5 Antibody, clone 5F7
Alternate Names	Anoctamin-5 Gnathodiaphyseal dysplasia 1 protein Transmembrane protein 16E
Background Information	Anoctamin-5 (UniProt Q75V66; also known as Gnathodiaphyseal dysplasia 1 protein, Integral membrane protein GDD1, Transmembrane protein 16E) is encoded by the ANO5 (also known as GDD1, LGMD2L, TMEM16E) gene (Gene ID 203859) in human. The human Anoctamins (ANO) compose a family of at least ten proteins, all exhibiting eight transmembrane domains and a DUF590 domain of unknown function. The ANO1, ANO2, ANO6, ANO8, and ANO9 are characterized to code for the elusive calcium-activated chloride channels (CaCCs). While the physiological function of anoctamin-5 is not yet characterized, dominant mutations in ANO5 have been found in the rare skeletal disorder gnathodiaphyseal dysplasia. In addition, recessive mutations in ANO5 are found to cause proximal muscular dystrophy (LGMD2L) and distal Miyoshi myopathy (MMD3).

References

Product Information
Format	Unpurified
Presentation	Mouse monoclonal IgMκ in buffer containing supernatant without preservatives.
Quality Level	MQ100

Applications
Application	This Anti-Anoctamin-5 Antibody, clone 5F7 is validated for use in Western Blotting for the detection of Anoctamin-5.
Key Applications	Western Blotting
Application Notes	Western Blotting Analysis: A 1:100 dilution from a representative lot detected Anoctamin-5 in 10 µg of mouse leg muscle tissue lysate.

Biological Information
Immunogen	Linear peptide corresponding to mouse Anoctamin-5 near the C-terminus.
Epitope	Near C-terminus
Clone	5F7
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Isotype	IgMκ
Species Reactivity	Mouse Human
Species Reactivity Note	Mouse, Human. Predicted to react with Rat based on 100% sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_998764
Gene Symbol	ANO5 GDD1 TMEM16E
Purification Method	Unpurified
UniProt Number	Q75V66
Molecular Weight	~37 kDa observed. ~37 kDa observed. Although the full-length protein has a calculated molecular weight of 107.2 kDa (Uniprot Q75V66), only a ~37 kDa band corresponding to a short spliced variant can be detected by Western blotting when using muscle tissue lysates. A band corresponding to the full-size protein and a 55 kDa band can be detected in addition to the 37 kDa band when using lysates prepared from human embryonal rhabdomyosarcoma (RMS) RD cells, C2C12 murine myoblasts, and fibroblasts.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in mouse skeletal muscle tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Anoctamin-5 in 10 µg of mouse skeletal muscle tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	200 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABS501	04055977175462

Documentation

Anti-Anoctamin-5 Antibody, clone 5F7 MSDS

职位
物料安全数据表 (MSDS)

Anti-Anoctamin-5 Antibody, clone 5F7 分析证书

标题	批号
Anti-Anoctamin-5, clone 5F7 -Q2595047	Q2595047

小册子

标题
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

技术信息

标题
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.

海报

标题
Post-Translational Modifications

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

此产品已添加到您的购物车。