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ABS1627 Sigma-AldrichAnti-CTGF Antibody, N-Term Antibody

This Anti-CTGF Antibody, N-Term Antibody is validated for use in Western Blotting, Neutralizing for the detection of CTGF.

Anti-CTGF Antibody, N-Term Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: CTGF, CCN2, HCS24, IGFBP8 UniProt Number: P29279

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, NEUT	Gt	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABS1627
Description	Anti-CTGF Antibody, N-Term Antibody
Alternate Names	Connective tissue growth factor CCN family member 2 Hypertrophic chondrocyte-specific protein 24 IBP-8 IGF-binding protein 8 IGFBP-8 Insulin-like growth factor-binding protein 8
Background Information	Connective tissue growth factor (UniProt P29279; also known as CCN family member 2, Hypertrophic chondrocyte-specific protein 24, IBP-8, IGF-binding protein 8, IGFBP-8, Insulin-like growth factor-binding protein 8) is encoded by the CTGF (also known as CCN2, HCS24, IGFBP8) gene (Gene ID 1490) in human. CTFG is one the three founding members of the CCN (Ctgf, Cyr61, and Nov) family of proteins involved in a wide array of cellular activities, including migration, proliferation, extracellular matrix synthesis, adhesion, survival, differentiation, and apoptosis. CTGF acts an autocrine mediator of transforming growth factor-beta (TGF-beta) in fibroblasts. CCN proteins are characterized by a more than 10% cysteine content and an absolute conservation of the position of their 38 cysteine residues. Human CTGF is synthesized with a signal peptide sequence (a.a. 1-26), the removal of which yields the mature protein with an N-terminal IGF-binding protein (IGFBP) domain (a.a. 27-98), followed by a von Willebrand factor type C (VWC or VWFC) domain (a.a. 101-167), a cysteine-free hinge region (a.a. 168-197), thrombospondin type-1 (TSP-1) domain (a.a. 198-243), and a C-terminal cysteine-knot or C-terminal CYS-KNOT (CTCK) motif (a.a. 256-330). Due to the high number of disulfide bridges, most of the CTGF domains are resistant to proteolytic digestion, and most proteases primarily cleave only in the cysteine-free hinge region, producing almost exclusively intact N- and C-terminal half fragments. The N-terminal half of CTGF is shown to mediate differentiation and collagen synthesis in concert with IGF-2, while its C-terminal half mediates cell proliferation in concert with EGF.

References

Product Information
Format	Affinity Purified
Presentation	Purified goat polyclonal antibody in buffer containing 0.3 M Glycine, 50 mM Tris, pH 8.0 without preservatives.
Quality Level	MQ100

Applications
Application	This Anti-CTGF Antibody, N-Term Antibody is validated for use in Western Blotting, Neutralizing for the detection of CTGF.
Key Applications	Western Blotting Neutralizing
Application Notes	Western Blotting Analysis: A representative lot detected the N-terminal (1-180), but not C-terminal (181-349), fragment derived from baculovirus expressed human CTGF by chymotrypsin cleavage. The N-terminal fragment appeared as a doublet due to differential glycosylation (Grotendorst, G.R., and Duncan, M.R. (2005). FASEB J. 19(7):729-738). Neutralizing Analysis: A representative lot inhibited CTGF-stimulated collagen synthesis, but not CTGF-stimulated DNA synthesis, in rat kidney (NRK) fibroblasts (Grotendorst, G.R., and Duncan, M.R. (2005). FASEB J. 19(7):729-738). Note: It is recommended that gel electrophoresis be performed under non-reducing condition to effectively separate CTGF N- and C-terminal fragments, as well as differentially glycosylated N-terminal fragments.

Biological Information
Immunogen	Recombinant full-length human CTGF.
Epitope	N-terminal half.
Concentration	Please refer to lot specific datasheet.
Host	Goat
Specificity	This polyclonal antibody was affinity-purified using the purified N-terminal fragment (1-180) from chymotrypsin-cleaved rhCTGF to eliminate immunoreactivity against the C-terminal half (181-349) of CTGF in the original goat antiserum raised against full-length rhCTGF (Grotendorst, G.R., and Duncan, M.R. (2005). FASEB J. 19(7):729-738).
Species Reactivity	Human
Species Reactivity Note	Human.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001892
Gene Symbol	CTGF CCN2 HCS24 IGFBP8
Purification Method	Affinity purified.
UniProt Number	P29279
Molecular Weight	35.92/38.09 kDa (isoform Long mature/pro-form), 18.95/16.35 kDa (isoform Long N-terminal fragment with/without a.a. 1-26 signal peptide), 33.04/35.21 kDa (isoform Short mature/pro-form) calculated. The N-terminal fragment (a.a. 1-180) generated by chymotrypsin cleavage of glycosylated CTGF can be resolved into a doublet under non-reducing condition with the upper band representing more glycosylated species.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting of chymotrypsin-cleaved CTGF fragments. Western Blotting Analysis: 2.0 µg/mL of this antibody detected 1 ng of the N-terminal (1-180), but not C-terminal (181-349), fragment derived from baculovirus expressed human CTGF by chymotrypsin cleavage.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ABS1627	04055977352894

Documentation

Anti-CTGF Antibody, N-Term Antibody MSDS

职位
物料安全数据表 (MSDS)

Anti-CTGF Antibody, N-Term Antibody 分析证书

标题	批号
Anti-CTGF, N-Term - 3202214	3202214
Anti-CTGF, N-Term - 3218761	3218761
Anti-CTGF, N-Term - 3240480	3240480
Anti-CTGF, N-Term - 3272234	3272234
Anti-CTGF, N-Term - 3425375	3425375
Anti-CTGF, N-Term - 4013440	4013440
Anti-CTGF, N-Term -Q2690236	Q2690236
Anti-CTGF, N-Term Polyclonal Antibody	3117819
Anti-CTGF, N-Term Polyclonal Antibody	2907823
Anti-CTGF, N-Term Polyclonal Antibody	3062434

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描述

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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