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05-1431 Sigma-AldrichAnti-Casein Kinase 2α Antibody, 1AD9

Anti-Casein Kinase 2α Antibody, 1AD9 detects level of Casein Kinase 2α & has been published & validated for use in IC, WB, IH(P) & IP.

Anti-Casein Kinase 2α Antibody, 1AD9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Signaling, Apoptosis & Cancer Research Sub-Category: Developmental Signaling Gene Symbol: CSNK2A1, CK2A1, CKII, CK II 3 EC 2.7.11.1 UniProt Number: P68400

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	ICC, WB, IH(P), IP	M	Purified	Monoclonal Antibody

Description
Catalogue Number	05-1431
Replaces	04-1129
Description	Anti-Casein Kinase 2α Antibody, 1AD9
Alternate Names	CK2 catalytic subunit alpha CKII alpha Casein kinase II subunit alpha Casein kinase 2, alpha 1 polypeptide Casein kinase II alpha 1 subunit Casein kinase II-alpha subunit Protein kinase CK2
Background Information	Unlike most serine/threonine kinases, Casein Kinases (CKs) are not activated by specific stimuli, but are essentially constitutively active. CK1 autophosphorylates, causing inactivation, and protein phosphatases reactivate the kinase. The substrates of CKs are numerous, but their function is less clear, perhaps due to the absence of obvious regulation. CK1 isoforms are thought to be involved in the response to DNA damage, while CK2 levels are elevated in many types of cancer.

References

Product Information
Format	Purified
Control	HeLa cell lysate
Presentation	Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate, pH 7.6 with 0.25 M NaCl and 0.1% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Casein Kinase 2α Antibody, 1AD9 detects level of Casein Kinase 2α & has been published & validated for use in IC, WB, IH(P) & IP.
Key Applications	Immunocytochemistry Western Blotting Immunohistochemistry (Paraffin) Immunoprecipitation
Application Notes	Immunoprecipitation: Recommended Immunocytochemistry: A previous lot of this antibody was used at a dilution of 5 μg/mL (acetone or methanol fix only). Optimal working dilutions must be determined by end user. Immunohistochemistry(paraffin): Representative testing from a previous lot. Optimal Staining of Casein Kinase 2 Monoclonal Antibody: S-KB-3 Cells

Biological Information
Immunogen	Purified recombinant α-subunit of casein kinase 2.
Epitope	Region common to both alpha and alpha' subunits
Clone	1AD9
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	The antibody binds specifically to the catalytic α-subunit of casein kinase2 (CK-2α). The antibody immunoprecipitates a protein with a molecular weight of 44 kD, CK-2α subunit. A 26 kD protein band, the β-subunit of the heterotetrameric holoenzyme α2β2, is coprecipitated with the CK-2α subunit. In western blots with extracts of kidney tissue, the antibody detects various protein bands between 40 and 44 kDa. Using 1 μg/mL of antibody, the lower detection limit for the holoenzyme is 500 ng in Western blot and 100 ng in dot blot; the detection limits for the α-subunit are 300 ng and 60 ng, respectively.
Isotype	IgG1
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001895.3
Entrez Gene Summary	Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene.
Gene Symbol	CSNK2A1 CK2A1 CKII CK II 3 EC 2.7.11.1
Purification Method	Purified
UniProt Number	P68400
UniProt Summary	FUNCTION: SwissProt: P68400 # Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation. SIZE: 391 amino acids; 45144 Da SUBUNIT: Tetramer composed of an alpha chain, an alpha' and two beta chains. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with RNPS1. SIMILARITY: SwissProt: P68400 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. & Contains 1 protein kinase domain
Molecular Weight	44 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on PC12 lysates. Western Blot Analysis: 1:500 dilution of this lot detected Neurofilament on 10 μg of PC12 lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
05-1431	04053252319846

Documentation

Anti-Casein Kinase 2α Antibody, 1AD9 MSDS

职位
物料安全数据表 (MSDS)

Anti-Casein Kinase 2α Antibody, 1AD9 分析证书

标题	批号
Anti-Casein Kinase 2#945;, clone 1AD9 - 2137043	2137043
Anti-Casein Kinase 2, clone 1AD9 - 2035080	2035080
Anti-Casein Kinase 2, clone 1AD9 - NG1577356	NG1577356
Anti-Casein Kinase 2, clone 1AD9 - NG1661010	NG1661010
Anti-Casein Kinase 2, clone 1AD9 - NG1734966	NG1734966
Anti-Casein Kinase 2, clone 1AD9 - NG1843413	NG1843413
Anti-Casein Kinase 2, clone 1AD9 - NG1895546	NG1895546
Anti-Casein Kinase 2α, clone 1AD9 - 2420444	2420444
Anti-Casein Kinase 2α, clone 1AD9 - 2436370	2436370
Anti-Casein Kinase 2α, clone 1AD9 - 3234583	3234583

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描述

Anti-Casein Kinase 2 Antibody

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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