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MAB427-C Sigma-AldrichAnti-Cav1.1 alpha1s Antibody, clone 1A, Ascites Free

Anti-Cav1.1 alpha1s Antibody, clone 1A, Ascites Free is an antibody against Cav1.1 alpha1s for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry, Immunofluorescence, Immunoprecipitation.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: CACNA1S, CACH1, CACN1, CACNL1A3 UniProt Number: P07293

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, Rb, Pl	WB, IH(P), ICC, IF, IP	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MAB427-C
Description	Anti-Cav1.1 alpha1s Antibody, clone 1A, Ascites Free
Alternate Names	Voltage-dependent L-type calcium channel subunit alpha-1S Calcium channel, L type alpha-1 polypeptide, isoform 3, skeletal muscle Voltage-gated calcium channel subunit alpha Cav1.1
Background Information	Voltage-dependent L-type calcium channel subunit alpha-1S (UniProt P07293; also known as Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle, Voltage-gated calcium channel subunit alpha Cav1.1) is encoded by the CACNA1S (also known as CACH1, CACN1, CACNL1A3) gene (Gene ID 100009585) in Oryctolagus cuniculus (rabbit) species. Voltage-dependent calcium channels (VDCC) are found in the membrane of excitable cells (e.g., muscle, glial cells, neurons) and exhibit 1000-fold greater permeability to calcium than to sodium under normal physiological conditions. There exist five types of VDCCs, including four high voltage-activated channels (Neural- or N-type channel, Residual- or R-type channel, Purkinje- or P/Q-type channel, and the dihydropyridine-sensitive Long-lasting or L-type channels) and the low voltage-activated Transient or T-type calcium channels. VDCCs exist as a complex of different subunits (α1, α2δ, β1-4, and γ), with α1 being the the ion-conducting pore-forming subunit that contains voltage-sensing machinery and the drug/toxin-binding sites. There exist ten genes coding for different α1 subunits, four of which code for L-type alpha-1 subunits (alpha-1A/Cav2.1/CACNA1A, alpha-1C/Cav1.2/CACNA1C, alpha-1D/Cav1.3/CACNA1D, and alpha-1S/Cav1.1/CACNA1S). The dihydropyridine-sensitive L-type channels are responsible for excitation-contraction coupling of skeletal, smooth, and cardiac muscle and for hormone secretion in endocrine cells. The α1S subunit (Cav1.1) contains the characteristic four homologous I–IV domains (a.a. 38-337, 418-664, 786-1068, 1105-1384) with six transmembrane α-helices each, having both its N- and C-terminal ends exposed intracellularly (a.a. 1-51, 1382-1873).

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ antibody in buffer containing PBS without preservatives.
Quality Level	MQ100

Applications
Application	Anti-Cav1.1 alpha1s Antibody, clone 1A, Ascites Free is an antibody against Cav1.1 alpha1s for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry, Immunofluorescence, Immunoprecipitation.
Key Applications	Western Blotting Immunohistochemistry (Paraffin) Immunocytochemistry Immunofluorescence Immunoprecipitation
Application Notes	Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cav1.1 alpha1s in 10 µg of HEK 293 and Raji membrane extracts. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Cav1.1 alpha1s in human heart and rat skeletal muscle tissue sections. Immunocytochemistry Analysis: Representative lots detected Cav1.1 localization by fluorescent immunocytochemistry staining of paraformaldehyde-fixed and Triton X-100-permeabilized mouse C2C12 myotubes as well as myotubes from primary mouse fetal skeletal myoblasts in culture (Couchoux, H., et al. (2011). Int .J. Biochem. Cell Biol. 43(5):713-720; Couchoux, H., et al. (2007). J. Physiol. 580(Pt.3):745-754). Immunocytochemistry Analysis: A representative lot detected an upregulated Cav1.1 (α1s) surface expression on differentiating H9C2 rat ventricular myoblasts by fluorescent immunocytochemistry with or without cell permeabilization (Ménard, C., et al. (1999). J. Biol. Chem. 274(41):29063-29070). Immunofluorescence Analysis: A representative lot immunostained T-tubule membrane in rat soleus muscle longitudinal cryosections by fluorescent immunohistochemistry (Zanin, M., et al. (2008). Am. J. Physiol. Cell Physiol. 294(1):C36-C46). Immunohistochemistry Analysis: A representative lot immunostained the raphide and druse crystal idioblasts in paraffin-embedded sections of young Pistia stratiotes leaves (Volk, G.M., et al. (2004). Ann. Bot. 93(6):741-753). Immunoprecipitation Analysis: A representative lot immunoprecipitated L-type Ca2+ channel Cav1.1 from mouse extensor digitorum longus muscle fiber homogenates (Delbono, O., et al. (1997). J. Neurosci. 17(18):6918-6928). Immunoprecipitation Analysis: A representative lot immunoprecipitated dihydropyridine PN200-100-binding complex from rabbit skeletal muscle transverse tubul (T-tubule) membrane preparation with an estimated affinity of 5 nM (Morton, M. E., and Froehner, S. C. (1987). J. Biol. Chern. 262(25):11904-11907). Function Analysis: A representative lot, when applied externally to BC3H1 mouse muscle cells, attentuated the slowly-activating, DHP-sensitive, high-threshold calcium current in a concentration-dependent manner (Morton, M.E., et al. (1988). J. Biol. Chem. 263(2):613-616). Immunoaffinity Purification Analysis: A representative lot was conjugated to resins and used to further purify the dihydropyridine (DHP) PN200-100-binding complex isolated by wheat germ agglutinin (WGA) chromatography from rabbit skeletal muscle transverse tubul (T-tubule) membrane preparation (Morton, M. E., and Froehner, S. C. (1987). J. Biol. Chern. 262(25):11904-11907). Western Blotting Analysis: Representative lots detected Cav1.1 alpha 1 subunit in skeletal & hart muscle tissues from rats and mice, as well as as well as in differentiating mouse C2C12 and rat H9C2 myoblasts (Esposito, A., et al. (2007). J. Appl. Physiol. 102(4):1640-1648; Bidaud, I., et al. (2006). J. Muscle Res. Cell Motil. 27(1):75-81; Ménard, C., et al. (1999). J. Biol. Chem. 274(41):29063-29070; Delbono, O., et al. (1997). J. Neurosci. 17(18):6918-6928). Western Blotting Analysis: A representative lot detected a ~180 kDa Ca2+ channel‐like protein in the microsomal membrane preparation from Pistia stratiotes leaves (Volk, G.M., et al. (2004). Ann. Bot. 93(6):741-753). Western Blotting Analysis: A representative lot detected the alpha 1 subunit of dihydropyridine receptor (DHPR) in rat tibialis anterior (TA) and rabbit hip adductor muscle extracts (Damiani, E., et al. (1996). Cell Calcium. 19(1):15-27). Western Blotting Analysis: A representative lot detected a ~210 kDa target band from BC3H1 mouse muscle cell membrane extract (Morton, M.E., et al. (1988). J. Biol. Chem. 263(2):613-616). Western Blotting Analysis: A representative lot detected a ~200 kDa target band under both reducing or non-reducing conditions using the dihydropyridine (DHP) PN200-100-binding complex isolated from rabbit skeletal muscle transverse tubul (T-tubule) membrane preparation by wheat germ agglutinin (WGA) column (Morton, M. E., and Froehner, S. C. (1987). J. Biol. Chern. 262(25):11904-11907).

Biological Information
Immunogen	Dihydropyridine-binding complex isolated from rabbit muscle t-tubules.
Epitope	Extracellular domain.
Clone	1A
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone 1A targets the extracellular region of voltage-dependent L-type calcium channel subunit alpha-1S or Cav1.1 encoded by the CACNA1S gene.
Isotype	IgG1κ
Species Reactivity	Human Mouse Rabbit Green Plants
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001095190
Gene Symbol	CACNA1S CACH1 CACN1 CACNL1A3
Purification Method	Protein G Purified
UniProt Number	P07293
Molecular Weight	~212 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in Jurkat membrane extract. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cav1.1 alpha1s in 10 µg of Jurkat membrane extract.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MAB427-C	04055977334258

Documentation

Anti-Cav1.1 alpha1s Antibody, clone 1A, Ascites Free MSDS

职位
物料安全数据表 (MSDS)

Anti-Cav1.1 alpha1s Antibody, clone 1A, Ascites Free 分析证书

标题	批号
Anti-Cav1.1 alpha1s, clone 1A, -Q2668257	Q2668257
Anti-Cav1.1 alpha1s, clone 1A, Ascites Free - 3477805	3477805
Anti-Cav1.1 alpha1s, clone 1A, Ascites Free - 3728259	3728259
Anti-Cav1.1 alpha1s, clone 1A, Ascites Free - 4038974	4038974

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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