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MAB13170 Sigma-AldrichAnti-Cav1.2 calcium channel Antibody, clone L57/46

This Anti-Cav1.2 calcium channel Antibody, clone L57/46 is validated for use in WB, IH for the detection of Cav1.2 calcium channel.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
小册子

Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: CACH2, CACN2, TS, CACNL1A1, CaV1.2, CCHL1A1, Cav1.2 UniProt Number: P15381

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H, R, Rb	WB, IHC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MAB13170
Description	Anti-Cav1.2 calcium channel Antibody, clone L57/46
Alternate Names	calcium channel, voltage-dependent, L type, alpha 1C subunit1 voltage-gated calcium channel alpha subunit Cav1.2 calcium channel, L type, alpha 1 polypeptide, isoform 1, cardic muscle calcium channel, cardic dihydropyridine-sensitive, alpha-1 subunit voltage-gated L-type calcium channel Cav1.2 alpha 1 subunit, splice variant 10* DHPR, alpha-1 subunit Voltage-gated calcium channel subunit alpha Cav1.2 Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle
Background Information	This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Many alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

References

Product Information
Format	Purified
Control	Mouse brain tissue lysate
Presentation	Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-Cav1.2 calcium channel Antibody, clone L57/46 is validated for use in WB, IH for the detection of Cav1.2 calcium channel.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect Cav1.2 calium channel in human kidney tissue.

Biological Information
Immunogen	Recombinant protein corresponding to rabbit Cav1.2 calcium channel at the cytoplasmic domain proximal to the C-terminus.
Epitope	Cytoplasmic domain
Clone	L57/46
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes Cav1.2 calcium channel at the cytoplasmic domain.
Isotype	IgG2bκ
Species Reactivity	Mouse Human Rat Rabbit
Species Reactivity Note	Demonstrated to react with mouse and human. Predicted to react with rat and rabbit based on 100% sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001129994
Gene Symbol	CACH2 CACN2 TS CACNL1A1 CaV1.2 CCHL1A1 Cav1.2
Purification Method	Protein G Purified
UniProt Number	P15381
UniProt Summary	FUNCTION: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function (By similarity). SUBUNIT STRUCTURE: Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with CABP1 and CACNA2D4 By similarity. Interacts (via C-terminal CDB motif) with CABP5; in a calcium-dependent manner (By similarity). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Cell membrane. Note: The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane. TISSUE SPECIFICTY: Expression in cardiac muscle. In lung, expressed in airway and vascular smooth muscle cells. DOMAIN: Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel. PTM: Phosphorylation by PKA activates the channel. SEQUENCE SIMILARITIES: Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. (View classification).
Molecular Weight	Observed at ~ 240 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in mouse brain tissue lysate. Western Blot Analysis: 2 µg/mL of this antibody detected Cav1.2 calcium channel on 10 µg of mouse brain tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MAB13170	04053252518690

Documentation

Anti-Cav1.2 calcium channel Antibody, clone L57/46 MSDS

职位
物料安全数据表 (MSDS)

Anti-Cav1.2 calcium channel Antibody, clone L57/46 分析证书

标题	批号
Anti-Cav1.2 calcium channel, clone L57/46 - 2127821	2127821
Anti-Cav1.2 calcium channel, clone L57/46 - 2394046	2394046
Anti-Cav1.2 calcium channel, -2550884	2550884
Anti-Cav1.2 calcium channel, -2579911	2579911
Anti-Cav1.2 calcium channel, -2667314	2667314
Anti-Cav1.2 calcium channel, -2716676	2716676
Anti-Cav1.2 calcium channel, -2727221	2727221
Anti-Cav1.2 calcium channel, -2762311	2762311
Anti-Cav1.2 calcium channel, -2807063	2807063
Anti-Cav1.2 calcium channel, clone L57/46	3030288

小册子

标题
Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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