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Detection of human DEFB4 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with partial recombinant human DEFB4 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-DEFB4 Mouse mAb (4C4) (Cat. No. ST1568) (1-5 µg/ml). Detection: chemiluminescence.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids 24-65 of human DEFB4, expressed as a GST fusion protein
Clone
4C4
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-November-2010 RFH
Synonyms
Anti-Defensin β4
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of human DEFB4 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with partial recombinant human DEFB4 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-DEFB4 Mouse mAb (4C4) (Cat. No. ST1568) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the DEFB4 protein.
Background
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids 24-65 of human DEFB4, expressed as a GST fusion protein
Clone
4C4
Isotype
IgG₁
Species
human
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
