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Anti-Dopamine D₃ Receptor, rabbit polyclonal, recognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, FC, and IHC.
More>>Anti-Dopamine D₃ Receptor, rabbit polyclonal, recognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, FC, and IHC. Less<<
Anti-Dopamine D₃ Receptor Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ELISA (see comments) Flow Cytometry (1:100, see application references) Immunoblotting (1:500) Immunohistochemistry (1:5000)
Application Comments
This antiserum is highly specific for the immunizing peptide sequence. For immunohistochemistry, a 1:5000 dilution of the reconstituted serum demonstrated staining of specific cells in various regions of rat brain sections. Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
a synthetic peptide (ASLSQLSSHC) corresponding to amino acids (2-10) of human dopamine D₃ receptor
Immunogen
Human
Host
Rabbit
Isotype
IgG
Species Reactivity
Human
Rat
Antibody Type
Polyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
27-July-2007 RFH
Application
ELISA (see comments) Flow Cytometry (1:100, see application references) Immunoblotting (1:500) Immunohistochemistry (1:5000)
Description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~50 kDa D3 dopamine receptor protein.
Host
Rabbit
Immunogen species
Human
Immunogen
a synthetic peptide (ASLSQLSSHC) corresponding to amino acids (2-10) of human dopamine D₃ receptor
Isotype
IgG
Species
human, rat
Positive control
Rat brain
Form
Lyophilized
Formulation
Undiluted serum.
Preservative
None
Comments
This antiserum is highly specific for the immunizing peptide sequence. For immunohistochemistry, a 1:5000 dilution of the reconstituted serum demonstrated staining of specific cells in various regions of rat brain sections. Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Toxicity
Standard Handling
References
Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
Application references
Flow Cytometry
McKenna, F., et al. 2002. J. Neuroimmunol.132, 34.
