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Anti-FGF21, mouse monoclonal, clone 2F11, recognizes the FGF21 protein in human tonsil tissue. It is validated for use in ELISA, Western blotting, and Immunohistochemistry (paraffin sections).
More>>Anti-FGF21, mouse monoclonal, clone 2F11, recognizes the FGF21 protein in human tonsil tissue. It is validated for use in ELISA, Western blotting, and Immunohistochemistry (paraffin sections). Less<<
Anti-FGF21 Mouse mAb (2F11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (3 µg/ml)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
Full-length, recombinant human FGF21 (aa 30-210), expressed as a GST fusion protein
Clone
2F11
Host
Mouse
Isotype
IgG1
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-November-2010 RFH
Synonyms
Anti-Fibroblast Growth Factor 21
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (3 µg/ml)
Application Data
Detection of human FGF21 by immunoblotting. Sample: Full-length, recombinant human protein. Primary antibody: Anti-FGF21 Mouse mAb (2F11) (Cat. No. ST1615) (1-5 µg/ml). Detection: chemiluminescence. Detection of human FGF21 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human tonsil tissue. Primary antibody: Anti-FGF21 Mouse mAb (2F11) (Cat. No. ST1615) (3 µg/ml). Detection: colorimetric (DAB).
Description
Purified mouse monoclonal antibody. Recgonizes the FGF21 protein.
Background
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined.
Host
Mouse
Immunogen
Full-length, recombinant human FGF21 (aa 30-210), expressed as a GST fusion protein
Clone
2F11
Isotype
IgG1
Species
human
Positive control
Human tonsil tissue
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
