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06-720-25UG Anti-HDAC1

Use Anti-HDAC1 Antibody (Rabbit Polyclonal Antibody) validated in EA, ICC, IP, WB to detect HDAC1 also known as histone deacetylase 1.

Anti-HDAC1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HDAC1, DKFZp686H12203, HD1, RPD3L1, GON-10, RPD3 UniProt Number: Q13547
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06-720-25UG
25 μg  
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      概述

      Replacement Information

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      重要规格表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MEnzyme Assays, ICC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-720-25UG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-HDAC1
      Alternate Names
      • histone deacetylase 1, reduced potassium dependency, yeast homolog-like 1
      Background InformationMammalian HADC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all are related to the yeast transcriptional regulator Rpd3p, are identified as histone deacetylases. The deacetylation of histones is associated with transcriptional silencing.
      References
      Product Information
      FormatPurified
      Control
      • NIH/3T3 cell extract
        Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 Cell Lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, containing 0.05% sodium azide. Frozen at -20ºC.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-HDAC1 Antibody (Rabbit Polyclonal Antibody) validated in EA, ICC, IP, WB to detect HDAC1 also known as histone deacetylase 1.
      Key Applications
      • Enzyme Assays
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      5 μg of a previous lot immunoprecipitated HDAC1 from 500 μg of 3T3/A31 RIPA lysate.

      Immunocytochemistry:
      This antibody has been reported to immunostain HDAC1 in 1% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 3T3 cells.

      HDAC Activity:
      This antibody has been reported to immunoprecipitate active HDAC.
      Biological Information
      Immunogen6X-His-tagged fragment representing amino acids 53-482 of mouse histone deacetylase 1 (HDAC1).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityRecognizes and is specific for HDAC1, Mr 65 kDa. May detects a non-specific protein at ~35 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteMouse, rat and human. Other species cross-reactivity not tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq]
      Gene Symbol
      • HDAC1
      • DKFZp686H12203
      • HD1
      • RPD3L1
      • GON-10
      • RPD3
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13547 # Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
      SIZE: 482 amino acids; 55103 Da
      SUBUNIT: Part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Associates with the 9-1-1 complex; interacts with HUS1. Found in a complex with DNMT3A and HDAC7. Interacts with BCOR, BRMS1L, DAXX, DNMT1, EP300, HCFC1, PCAF, PHB2, MIER1, JMJD2A/JHDM3A, MINT, NRIP1, RERE, SETDB1, SUV39H1, TGIF, TGIF2, UHRF1 and UHRF2. Interacts with the non-histone region of H2AFY. Interacts with HDAC9. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts with BANP, CBFA2T3 and JARID1B. Interacts with SAP30L.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
      PTM: Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1. & Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
      SIMILARITY: SwissProt: Q13547 ## Belongs to the histone deacetylase family. Type 1 subfamily.
      Molecular Weight65 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on RIPA lysates from 3T3/A31 cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected HDAC1 in RIPA lysates from 3T3/A31 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      产品目录编号 GTIN
      06-720-25UG 04054839337529