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			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OB05 Sigma-AldrichAnti-Histidine-Tagged Protein Mouse mAb (13/45/31/2)

Anti-Histidine-Tagged Protein, mouse monoclonal, clone 13/45/31/2, specifically recognizes an epitage of 6 consecutive His residues. It is validated for use in ELISA, WB, IF & IP.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Anti-His6

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
A	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Specifically recognizes an epitage of six consecutive His residues of both natural and recombinant sources.
Catalogue Number	OB05
Brand Family	Calbiochem®
Synonyms	Anti-His6
Application Data	Detection of human histidine-tagged protein by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) (Cat. No. OB05) (1:100). Detection: chemiluminescence. Detection of human histidine-tagged protein by immunofluorescence. Samples: Transfected HeLa cells from the plasmid expression pCMV-His6-GFP-APKD1-5a with GFP autofluorescence (left figure) and primary antibody histidine-tagged protein, secondary antibody Cy3-conjugated anti-mouse (right figure). Primary antibody: Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) (Cat. No. OB05) (1:100). Detection: fluorescence.

References
References	Zentgraf, H., et al. 1995. Nucl. Acids. Res. 23, 3347. Gu, J., et al. 1994. BioTechniques 17, 257. Sporeno, E., et al. 1994. J. Biol. Chem., 10991. Sisk, W.P., et al. 1994. J. Virol. 68, 766. Lu, T., et al. 1993. Anal. Biochem. 213, 318. Garner, J., et al. 1992. Cell 69, 833. Hochuli, E., et al. 1987. J. Chromatogr. 411, 177.

Product Information
Declaration	Manufactured by Dianova, GmbH, Germany.
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive control	Proteins containing a His•Tag^® sequence
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation
Application Notes	ELISA (0.1-0.4 µg/ml) Immunoblotting (1-4 µg/ml) Immunofluorescence (1-4 µg/ml) Immunoprecipitation (4 µg/ml)
Application Comments	Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a recombinant protein containing a His•Tag^® sequence
Epitope	HHHHHH
Clone	13/45/31/2
Host	Mouse
Isotype	IgG₁
Species Reactivity	All
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
OB05-100UGCN	04055977210057

Documentation

Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) MSDS

职位
物料安全数据表 (MSDS)

Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) 分析证书

标题	批号
OB05	输入批号

参考

参考信息概述
Zentgraf, H., et al. 1995. Nucl. Acids. Res. 23, 3347. Gu, J., et al. 1994. BioTechniques 17, 257. Sporeno, E., et al. 1994. J. Biol. Chem., 10991. Sisk, W.P., et al. 1994. J. Virol. 68, 766. Lu, T., et al. 1993. Anal. Biochem. 213, 318. Garner, J., et al. 1992. Cell 69, 833. Hochuli, E., et al. 1987. J. Chromatogr. 411, 177.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	22-January-2009 JSW
Synonyms	Anti-His6
Application	ELISA (0.1-0.4 µg/ml) Immunoblotting (1-4 µg/ml) Immunofluorescence (1-4 µg/ml) Immunoprecipitation (4 µg/ml)
Application Data	Detection of human histidine-tagged protein by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) (Cat. No. OB05) (1:100). Detection: chemiluminescence. Detection of human histidine-tagged protein by immunofluorescence. Samples: Transfected HeLa cells from the plasmid expression pCMV-His6-GFP-APKD1-5a with GFP autofluorescence (left figure) and primary antibody histidine-tagged protein, secondary antibody Cy3-conjugated anti-mouse (right figure). Primary antibody: Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) (Cat. No. OB05) (1:100). Detection: fluorescence.
Description	Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes a His•Tag^® sequence in native and recombinant proteins. Specifically recognizes an epitope of six consecutive His residues of both natural and recombinant sources.
Background	Identification of proteins for which there does not exist antibodies has traditionally been overcome by the use of epitope tagging systems in which expression vectors containing an open reading frame encoding a small fragment of a specific protein (for which an antibody exists) together with multiple cloning sites are used to express foreign genes. Several different epitope tagging systems of this type currently in use include maltose-binding-protein (MBP), glutathione S-transferase (GST), TrpE, and myc. Tags are generally placed at either the N-or C-terminus of the protein as epitope tags placed within a coding sequence of a target protein increases the possibility of interference with the function of the protein and also decreases ability of tag-specific antibody to detect epitope tagged sequence buried by native folding of the target protein. A second type of epitope tagging involves the addition of multiple histidine residues, usually six residues, at either the N- or C-terminus of the target protein. Expressed proteins with such a tag may then be purified by binding to divalent metal ion columns, most commonly to chelated-Ni²⁺ columns using a technique known as IMAC or immobilized metal ion chromatography. This type of epitope tagging is particularly suited for the purification of recombinant proteins from E. coli expression systems. Analysis of histidine-tagged proteins by immunoblotting or immunofluorescence may also be done using an antibody to the histidine tag itself, thereby eliminating the need for an antibody specific for the protein in question.
Host	Mouse
Immunogen	a recombinant protein containing a His•Tag^® sequence
Epitope	HHHHHH
Clone	13/45/31/2
Isotype	IgG₁
Species	all His•Tag^® proteins
Positive control	Proteins containing a His•Tag^® sequence
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Zentgraf, H., et al. 1995. Nucl. Acids. Res. 23, 3347. Gu, J., et al. 1994. BioTechniques 17, 257. Sporeno, E., et al. 1994. J. Biol. Chem., 10991. Sisk, W.P., et al. 1994. J. Virol. 68, 766. Lu, T., et al. 1993. Anal. Biochem. 213, 318. Garner, J., et al. 1992. Cell 69, 833. Hochuli, E., et al. 1987. J. Chromatogr. 411, 177.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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